PCDH-CMV-mCherry-TRIM9

LOCUS      PCDH-CMV-mCherry-TRIM9             9285 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3215..3257
                     /label=mcherry-TRIM9-F
     misc_feature    3240..5372
                     /label=9 long
     primer_bind     complement(3713..3723)
                     /label=mcherry-TRIM9-R
     primer_bind     3769..3786
                     /label=T9-seqF1
     primer_bind     complement(5350..5397)
                     /label=mcherry-TRIM9-R
     primer_bind     complement(5369..5397)
                     /label=TRIM9s-R
     misc_feature    5382..5969
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5853..5864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6042..6275
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6347..6468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6487..6622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6660..6676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6684..6700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6708..6738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6753..6774
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7062..7650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7821..8681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8682..8786)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9155..9168)
                     /label=Primer 5
     primer_bind     9260..9276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggaggagat ggaagaggag ttgaaatgcc ccgtgtgcgg ctccttctat cgggagccca
     3301 tcatcctgcc ctgctctcac aatttgtgtc aggcgtgcgc ccgcaacatc ctggtgcaga
     3361 ccccagagtc tgaatccccc cagagccatc gggccgcggg ctccggggtc tccgactatg
     3421 actatctgga cctggacaag atgagcctat acagcgaggc ggacagcggc tatggctcct
     3481 acggggggtt cgccagcgcc cccactaccc cgtgccagaa gtcccccaac ggcgtccgcg
     3541 tgtttccccc ggctatgccg ccaccggcca cccacttgtc accggccctg gccccggtgc
     3601 cccgcaactc ctgtatcacc tgcccccagt gtcaccgcag cctcatcctg gatgaccggg
     3661 ggctccgcgg cttccccaag aatcgcgtac tggaaggggt aattgaccgc taccagcaga
     3721 gcaaagccgc ggccctcaag tgccagctct gcgagaaggc gcccaaggaa gccaccgtca
     3781 tgtgcgaaca gtgcgatgtc ttctactgcg atccgtgccg cctgcgctgc cacccgcccc
     3841 gggggcccct agccaagcac cgcctggtgc ccccggccca gggtcgtgtg agccggaggc
     3901 tgagcccacg caaggtctcc acctgcacag accacgagct ggagaaccac agcatgtact
     3961 gcgtgcaatg caagatgccc gtgtgctacc agtgcttgga ggagggcaaa cactccagcc
     4021 acgaagtcaa ggctctgggg gccatgtgga aactacataa gagccagctc tcccaggcgc
     4081 tgaacggact gtcagacagg gccaaagaag ccaaggagtt tctggtacag ctgcgcaaca
     4141 tggtccagca gatccaggag aacagtgtgg agtttgaagc ctgtctggtg gcccaatgtg
     4201 atgccctcat cgatgccctc aacagaagaa aagcccagct gctggcccgc gtcaacaagg
     4261 agcatgagca caagctgaag gtggttcgag atcagatctc tcactgcaca gtgaaattgc
     4321 gccagaccac aggtctcatg gagtactgct tggaggtgat taaggaaaat gatcctagtg
     4381 gttttttgca gatttctgac gccctcataa gaagagtgca cctgactgag gatcagtggg
     4441 gtaaaggcac actcactcca aggatgacca cggactttga cttgagtctg gacaacagcc
     4501 ctctgctgca atccatccac cagctggatt tcgtgcaagt gaaagcttcc tctccagtcc
     4561 cagcaacccc tatcctacag ctggaggaat gttgtaccca caacaacagc gctacgttgt
     4621 cctggaaaca gccacctctg tccacggtgc ccgccgatgg atacattctg gagctggatg
     4681 atggcaacgg tggtcaattc cgggaggtgt atgtggggaa ggagacaatg tgcactgtgg
     4741 atggtcttca cttcaacagc acatacaacg ctcgggtcaa ggccttcaac aaaacaggag
     4801 tcagcccgta cagcaagacc ctggtcctcc aaacgtctga ggtggcctgg tttgctttcg
     4861 accctggctc ggcgcactcg gacatcatcc tctccaatga caacctgaca gtgacctgta
     4921 gtagctatga tgaccgggtg gtgctaggga agactggctt ctccaagggc atccactact
     4981 gggagctcac ggtagatcgc tatgacaacc accctgatcc tgcctttggt gtggctcgca
     5041 tggacgtgat gaaggatgtg atgttaggaa aagacgacaa agcttgggca atgtatgtgg
     5101 acaataaccg gagctggttc atgcacaaca actcgcacac caacagaact gagggaggga
     5161 tcacaaaagg ggccacaatt ggggtcctcc tcgacttaaa tagaaaaaac ttgacatttt
     5221 ttatcaacga tgaacaacaa ggtcccatag catttgataa cgtggagggc ctcttcttcc
     5281 ctgcggtcag cctgaacagg aacgtgcagg tcacgctgca caccgggctc ccagtccccg
     5341 acttctactc cagcagagca tcaatagcct aatagggatc caatcaacct ctggattaca
     5401 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5461 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5521 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5581 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5641 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5701 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5761 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5821 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5881 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga
     5941 gtcggatctc cctttggccg cctccccgcc ggtaccttta agaccaatga cttacaaggc
     6001 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6061 ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6121 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6181 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6241 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6301 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     6361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6481 ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6541 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6601 tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg
     6661 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6721 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6841 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6961 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7021 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7081 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7141 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7201 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7261 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7321 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7381 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7441 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7501 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7561 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7621 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7681 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7741 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7801 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7861 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7921 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7981 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8041 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8101 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8161 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8221 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8281 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8341 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8401 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8461 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8521 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8581 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8641 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8761 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     8821 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     8881 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     8941 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     9001 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     9061 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     9121 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     9181 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     9241 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//