| 质粒名称: | pSL2000 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002464 |
| 产品用途: | Mammalian PhCMV-driven pMag-VP64 (Magnet) expression vector (PhCMV-TetR-nMag-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | TetR-nMag |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL2000
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL2000载体质粒基本信息
pSL2000质粒图谱载体图谱和pSL2000载体序列质粒序列多克隆位点信息
pSL2000质粒载体简介
pSL2000质粒序列载体序列
LOCUS pSL2000 5995 bp DNA circular SYN 11-FEB-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5995)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 31, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5995
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 820..838
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 829..880
/label=oSCL3106
primer_bind 829..839
/label=T7
primer_bind 843..880
/label=oQZ109
/note="HindIII"
primer_bind 849..880
/label=oLZ135 NheI-pMag-L-Hind3 F
primer_bind 857..880
/label=oQZ101
/note="EcoRI-pMag"
misc_feature 858..1307
/label=pMag
primer_bind 858..880
/label=oSCL3100
primer_bind complement(1282..1343)
/label=oLZ136 NheI-pMag-L-Hind3 R
primer_bind complement(1282..1336)
/label=oQZ102
/note="KpnI-Linker-pMag"
primer_bind complement(1282..1327)
/label=oSCL3107
primer_bind complement(1282..1307)
/label=oSCL3101
primer_bind complement(1288..1316)
/label=oQZ097
/note="BamHI-pMag"
primer_bind complement(1291..1307)
/label=pMag R
/note="XbaⅠ"
primer_bind 1308..1333
/label=oSCL2101
primer_bind 1308..1333
/label=oSCL2966
primer_bind complement(1308..1327)
/label=oSCL3090
CDS 1309..1313
/codon_start=1
/label=AML19
/translation="S"
misc_feature 1312..1313
/label=TetR-CIBN
misc_feature 1312..1313
/label=tetr
primer_bind complement(1314..1363)
/label=oSCL264
primer_bind complement(1314..1344)
/label=oSCL244
CDS 1314..1343
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
misc_feature 1317..1327
/label=linker
primer_bind 1330..1344
/label=oSCL3100
primer_bind 1340..1358
/label=oXQ080
CDS 1344..1493
/label=VP64
misc_feature 1344..1493
/label=VPR
misc_feature 1344..1382
/label=VP16 minimal
primer_bind 1344..1358
/label=oSCL265
misc_feature 1383..1421
/label=VP16 minimal
misc_feature 1422..1460
/label=VP16 minimal
primer_bind complement(1479..1500)
/label=oSCL266
primer_bind complement(1479..1496)
/label=Primer 2
primer_bind complement(1479..1496)
/label=Primer 4
primer_bind complement(1479..1494)
/label=oXQ082
primer_bind complement(1552..1572)
/label=oSCL3101
primer_bind complement(1558..1577)
/label=Primer 2
polyA_signal 1595..1819
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1865..2293
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2307..2636
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2487..2622
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2703..3497
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3174..3205)
/label=oSCL3090
polyA_signal 3671..3792
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3841..3857)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3865..3881
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3889..3919)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3934..3955
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4243..4828)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4999..5859)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5860..5964)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct aatacgactc actatagggt
841 aaaagcttgc caccatgcat actctttatg cccccggtgg atatgacatt atgggatatc
901 tgaggcagat caggaaccgg ccaaacccgc aggtggaact gggccccgtg gatacatcct
961 gcgccttgat tctttgtgac ctgaaacaga aagacacccc gatagtttac gcgagtgaag
1021 ccttcctcta catgacaggt tacagcaacg cagaggtgct gggccggaat tgccggtttc
1081 tgcaaagccc tgacggcatg gtgaagccca agagcacccg gaagtacgtg gatagtaaca
1141 caatcaatac tatgcgcaag gcaatcgaca ggaatgccga ggtgcaggtt gaagtagtca
1201 attttaaaaa gaatggacag cgatttgtta atttcctgac tatgatacct gttagggacg
1261 aaacaggcga gtatcgatac tctatgggat tccagtgcga aacagaagct agcgccagcg
1321 gaagtggcgg aggaggcgac gtcgacgcat tggacgattt tgatctggat atgctgggaa
1381 gtgacgccct cgatgatttt gaccttgaca tgcttggttc ggatgccctt gatgactttg
1441 acctcgacat gctcggcagt gacgcccttg atgatttcga cctggacatg ctgtgagatc
1501 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
1561 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
1621 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
1681 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
1741 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
1801 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
1861 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
1921 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
1981 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
2041 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
2101 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
2161 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
2221 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
2281 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
2341 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
2401 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
2461 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
2521 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
2581 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
2641 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
2701 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
2761 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
2821 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
2881 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
2941 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
3001 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
3061 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
3121 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
3181 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
3241 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
3301 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
3361 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
3421 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
3481 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
3541 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
3601 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
3661 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
3721 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
3781 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
3841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
3901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
3961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
4021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
4081 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
4141 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
4261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
4321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
4441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
4501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
4561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
4621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4681 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
4801 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4861 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
4921 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
4981 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
5041 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
5101 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
5161 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
5221 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
5281 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
5341 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
5401 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
5461 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
5521 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
5581 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
5641 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
5701 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
5761 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
5821 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
5881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
5941 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
pSL2000载体图谱质粒图谱pdf版和pSL2000载体序列质粒序列等相关资料下载
pSL2000质粒载体应用举例
- 上一篇：pSL1999
- 下一篇：没有了
