| 质粒名称: | pSL1635 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002455 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for a LIRP variant with the LOV2 domain inserted between amino acids 77 and 78 of L7Ae (PhCMV-L7Ae(LOV2 77/78)-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
PhCMV
|
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | L7Ae(LOV2 77/78) |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1635
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1635载体质粒基本信息
pSL1635质粒图谱载体图谱和pSL1635载体序列质粒序列多克隆位点信息
pSL1635质粒载体简介
pSL1635质粒序列载体序列
LOCUS pSL1635 6197 bp DNA circular SYN 09-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6197)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 31, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6197
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 863..882
/label=T7
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 911..938
/label=oSCl008
primer_bind 921..944
/label=DDL7AeF
/note="KpnI"
misc_feature 923..1154
/direction=RIGHT
/note="note=""Fragment extracted from PCR(pWH132.xdna) [363
nt] : (BamHI[6] / XbaI[373])"
misc_feature 924..1154
/label=L7Ae
primer_bind 924..944
/label=L7Ae FLP
primer_bind 1059..1092
/label=oSCL2145
primer_bind complement(1059..1091)
/label=oSCL2139
misc_feature 1091^1092
/label=CS2
primer_bind complement(1092..1111)
/label=oSCL2146
primer_bind 1092..1109
/label=oSCL2140
primer_bind 1116..1175
/label=oSCL2143
primer_bind complement(1116..1133)
/label=oSCL2137
misc_feature 1133^1134
/label=CS1
primer_bind 1134..1154
/label=oSCL2138
primer_bind complement(1134..1154)
/label=oSCL2141
primer_bind complement(1134..1154)
/label=oSCL2144
primer_bind 1135..1175
/label=oSCL2147
misc_feature 1148..1154
/label=Coh2
CDS 1148..1154
/codon_start=1
/label=Coh2-N
/translation="VK"
misc_feature 1155..1568
/label=LOV2
primer_bind 1155..1175
/label=oSCL1757
primer_bind 1155..1175
/label=oSCL2145
primer_bind complement(1547..1588)
/label=oSCL2148
primer_bind complement(1547..1568)
/label=oSCL1758
primer_bind complement(1547..1568)
/label=oSCL2144
primer_bind complement(1547..1568)
/label=oSCL2146
misc_feature 1569..1694
/label=L7Ae
misc_feature 1569..1694
/direction=RIGHT
/note="note=""Fragment extracted from PCR(pWH132.xdna) [363
nt] : (BamHI[6] / XbaI[373])"
primer_bind 1569..1588
/label=oSCL2142
misc_feature 1569..1571
/label=Coh2
CDS 1569..1571
/codon_start=1
/label=Coh2-C
/translation="S"
misc_feature 1569
/label=CS3
primer_bind complement(1670..1694)
/label=DDL7AeR
/note="EcoRI"
primer_bind complement(1674..1703)
/label=L7Ae RLE
primer_bind complement(1676..1717)
/label=oSCL2256
primer_bind complement(1676..1694)
/label=oSCL009
primer_bind complement(1768..1786)
/label=oYW306
terminator 1794..2021
/label=bGH PA
polyA_signal 1797..2021
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2067..2495
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2509..2838
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2689..2824
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 2689..2766
/label=SV40 ORI
CDS 2905..3699
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2908..3699
/label=Kan/neoR
polyA_signal 3873..3994
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4039..4059)
/label=M13-rev
primer_bind complement(4043..4059)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4065..4087)
/label=LacO
protein_bind 4067..4083
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4091..4121)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4092..4121)
/label=lac
protein_bind 4136..4157
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4445..5030)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5201..6061)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(5204..5863)
/label=AmpR
promoter complement(6062..6166)
/gene="bla"
/label=AmpR promoter
promoter complement(6103..6131)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttcgcc accatgtacg tgcgcttcga ggtgcccgag gacatgcaga
961 acgaggccct gagcctgctg gaaaaagtgc gcgagagcgg caaagtgaag aagggcacca
1021 acgaaaccac caaggccgtg gaacggggcc tggccaagct ggtgtatatc gccgaggacg
1081 tggacccccc cgagattgtg gcccatctgc ccctgctgtg cgaagagaag aacgtgccct
1141 acatctacgt gaagggtttg gaacgtatcg aaaagaattt tgtcatcacg gacccgcgtc
1201 tgcccgacaa tccgattatc ttcgcgtcag actctttctt acaactgact gagtatagta
1261 gagaggagat attggggcgt aactgtagat ttcttcaggg gccagaaact gatcgggcta
1321 ccgttcgcaa gatacgtgac gcaatagaca accagaccga ggtgacggtg cagctgatta
1381 actacacaaa gtctgggaag aagttctgga acctgtttca tttacaacct atgagggacc
1441 aaaaaggtga cgttcaatat ttcatcgggg ttcagttaga tgggactgag cacgtgagag
1501 atgcagcaga aagagagggt gtaatgctta ttaaaaaaac agccgagaat atcgacgaag
1561 ccgctggttc caagaacgac ctgggcagag ccgtgggcat cgaggtgcca tgtgcctctg
1621 ccgccatcat caacgagggc gagctgcgga aagaactggg cagcctggtg gaaaagatca
1681 agggcctgca gaagtaagga tccactagtc cagtgtggtg gaattctgca gatatccagc
1741 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
1801 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
1861 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
1921 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
1981 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
2041 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
2101 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
2161 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
2221 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
2281 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
2341 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
2401 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
2461 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
2521 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2581 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2641 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
2701 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
2761 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2821 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
2881 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
2941 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
3001 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
3061 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
3121 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
3181 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
3241 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
3301 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
3361 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
3421 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
3481 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
3541 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
3601 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
3661 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
3721 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
3781 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
3841 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
3901 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
3961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
4021 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
4081 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
4141 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
4201 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
4261 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
4321 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
4381 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
4441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
4501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
4561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
4621 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
4681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
4741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
4801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
4861 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
4921 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
4981 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
5041 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
5101 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
5161 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
5221 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
5281 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
5341 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
5401 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
5461 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
5521 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
5581 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
5641 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
5701 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
5761 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
5821 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
5881 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
5941 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
6001 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
6061 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
6121 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
6181 aagtgccacc tgacgtc
//
pSL1635载体图谱质粒图谱pdf版和pSL1635载体序列质粒序列等相关资料下载
pSL1635质粒载体应用举例
- 上一篇：pSL1634
- 下一篇：没有了
