pQL67

LOCUS       pQL67              6414 bp DNA     circular SYN 17-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6414
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     213..249
                     /label=QL129
     primer_bind     complement(230..262)
                     /label=QL130
     misc_feature    234..241
                     /label=1
     misc_feature    248..395
                     /label=hcmvmin
     misc_feature    317..331
                     /label=New Feature
     misc_feature    418..441
                     /label=New Feature
     misc_feature    443..1963
                     /label=SEAP
     primer_bind     complement(1943..1971)
                     /label=Oscl23-2
     primer_bind     complement(1945..1963)
                     /label=Oscl13-2
     terminator      2011..2238
                     /label=bGH PA
     misc_feature    2906..2983
                     /label=SV40 ORI
     CDS             3125..3916
                     /label=Kan/neoR
     primer_bind     complement(4256..4276)
                     /label=M13-rev
     misc_binding    complement(4282..4304)
                     /label=LacO
     promoter        complement(4309..4338)
                     /label=lac
     CDS             complement(5421..6080)
                     /label=AmpR
     promoter        complement(6320..6348)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtcaggaaa
      241 tgctagctcg agctcggtac ccgggtcgag taggcgtgta cggtgggagg cctatataag
      301 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      361 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc tcgcccgggg
      421 atcctctaga gtcagcttct gcatgctgct gctgctgctg ctgctgggcc tgaggctaca
      481 gctctccctg ggcatcatcc cagttgagga ggagaacccg gacttctgga accgcgaggc
      541 agccgaggcc ctgggtgccg ccaagaagct gcagcctgca cagacagccg ccaagaacct
      601 catcatcttc ctgggcgatg ggatgggggt gtctacggtg acagctgcca ggatcctaaa
      661 agggcagaag aaggacaaac tggggcctga gatacccctg gccatggacc gcttcccata
      721 tgtggctctg tccaagacat acaatgtaga caaacatgtg ccagacagtg gagccacagc
      781 cacggcctac ctgtgcgggg tcaagggcaa cttccagacc attggcttga gtgcagccgc
      841 ccgctttaac cagtgcaaca cgacacgcgg caacgaggtc atctccgtga tgaatcgggc
      901 caagaaagca gggaagtcag tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc
      961 agccggcacc tacgcccaca cggtgaaccg caactggtac tcggacgccg acgtgcctgc
     1021 ctcggcccgc caggaggggt gccaggacat cgctacgcag ctcatctcca acatggacat
     1081 tgacgtgatc ctaggtggag gccgaaagta catgtttccc atgggaaccc cagaccctga
     1141 gtacccagat gactacagcc aaggtgggac caggctggac gggaagaatc tggtgcagga
     1201 atggctggcg aagcgccagg gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc
     1261 ttccctggac ccgtctgtga cccatctcat gggtctcttt gagcctggag acatgaaata
     1321 cgagatccac cgagactcca cactggaccc ctccctgatg gagatgacag aggctgccct
     1381 gcgcctgctg agcaggaacc cccgcggctt cttcctcttc gtggagggtg gtcgcatcga
     1441 ccatggtcat catgaaagca gggcttaccg ggcactgact gagacgatca tgttcgacga
     1501 cgccattgag agggcgggcc agctcaccag cgaggaggac acgctgagcc tcgtcactgc
     1561 cgaccactcc cacgtcttct ccttcggagg ctaccccctg cgagggagct ccatcttcgg
     1621 gctggcccct ggcaaggccc gggacaggaa ggcctacacg gtcctcctat acggaaacgg
     1681 tccaggctat gtgctcaagg acggcgcccg gccggatgtt accgagagcg agagcgggag
     1741 ccccgagtat cggcagcagt cagcagtgcc cctggacgaa gagacccacg caggcgagga
     1801 cgtggcggtg ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc aggagcagac
     1861 cttcatagcg cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg cctgcgacct
     1921 ggcgcccccc gccggcacca ccgacgccgc gcacccgggt taagcggccg ctcgagtcta
     1981 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     2041 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     2101 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     2161 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     2221 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     2281 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2341 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2401 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2461 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2521 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2581 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2641 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2701 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     2761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     2821 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2881 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2941 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     3001 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3061 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     3121 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3181 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3241 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3301 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3361 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3421 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3481 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3541 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3601 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     3661 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3721 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3781 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     3841 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3901 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     3961 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4021 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4081 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4141 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4201 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     4261 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4321 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4381 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4441 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4501 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4561 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4621 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4681 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4741 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4801 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4861 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4921 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4981 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5041 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5101 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5161 agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag
     5221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//