| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001634 |
| 产品用途: | 5' AAVLINK plasmid for CDK5RAP2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CDK5RAP2 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG 6760 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6760)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6760
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3473
/codon_start=1
/label=CDK5RAP2
/translation="GALPSTAAD*GWPWHLCHLPSTHEHHD*GI*GVAAGQ*CGLLCGR
GFPGTAESMC*AAGEIGKAISQRKISWSGNEHPE*TDGED*GRQLNLPTSSA*IS*AFS
LSCAL*L*NI*KVLLLTRPEAR**DREDFSYGEQFFSRLTNGTHTGNSNFEKAFRRIY*
NK*EATETVGTARI*ICSRFYKHFCFWFRAS*FSNIRNSFLEEAEPGPQCNAH*RIQR*
TEGE*QITRVPLQEDREPGAPSAGVCQREGRK*KAAERRQREGETQPAADPGGPLQRPG
AEQGAGGGEVEAAAALTE*QAIAVSPSGAEGV*EAG*RAQETERGVGRRLEGAGSFQGP
AQPPDGDPGSALATRKEHRNQQHSAEQAQGTAGKGGREGTGRSPHSGCPSRVHP*GAPS
A*QTRW*QISHGK**FI*SV*FLPGSDTKISFRDSSTLWE*HGLPLLRQWQFGN*HSVC
VPPGHWPPPVGQQEWPPCPGPD*GL*GPAQTDQPGTEAPC*NGHSNPRGSQLHKSRAGN
KGSTPSTTEQVCEQCEHGQADPGRGLQAAEASLESLTPRGWPVPPSL*ADWRNEGRGHQ
TT*KIV*TRKEVAKHHEAFAAEQAPGKSHL*SIGRNPQNPSEGQRKPGA*AWGSPSRNM
QSQQTRL"
misc_feature 2365..2370
/label=SDS
misc_feature 2404..2409
/label=SDS
misc_feature 2407..2412
/label=SDS
misc_feature 2573..2578
/label=SDS
CDS 3480..3545
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3549..3980
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3693..3698
/label=SDS
repeat_region 4023..4163
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4023..4152
/label=AAV2 ITR
rep_origin 4238..4693
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4975..5079
/gene="bla"
/label=AmpR promoter
CDS 5080..5940
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6111..6699
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggag ctctcccttc
1561 aacggcagca gattaaggat ggccatggca tctgtgtcat ctcccgtcaa cacatgaaca
1621 ccatgattaa ggcatttgag gagttgctgc aggccagtga tgtggattac tgtgtggccg
1681 agggtttcca ggaacagctg aatcaatgtg ctgagctgct ggagaaattg gaaaagctat
1741 ttctcaacgg aaaatcagtt ggagtggaaa tgaacaccca gaatgaactg atggagagga
1801 ttgaggaaga caacttaacc taccaacatc ttctgcctga atctcctgag ccttcagcct
1861 ctcatgcgct ctctgattat gaaacatctg aaaagtcctt cttctcacga gaccagaagc
1921 aagataatga gacagagaag acttcagtta tggtgaacag tttttctcaa gacttactaa
1981 tggaacacat acaggaaatt cgaactttga gaaagcgttt agaagaatct attaaaacaa
2041 atgagaagct acggaaacag ttggaacggc aaggatctga atttgttcaa ggttctacaa
2101 gcatttttgc ttctggttca gagcttcata gttctctaac atcagaaatt catttcttga
2161 ggaagcagaa ccaggccctc aatgcaatgc tcattaaagg atccagagat aaacagaagg
2221 agaatgacaa attacgagag tccctctcca ggaagaccgt gagcctggag caccttcagc
2281 gggagtatgc cagcgtgaag gaagaaaatg aaaggctgca gaaagaaggc agcgagaagg
2341 agagacacaa ccagcagctg atccaggagg tccgctgcag cggccaggag ctgagcaggg
2401 tgcaggagga ggtgaagttg aggcagcagc tgctctcaca gaatgacaag ctattgcagt
2461 ctctccgagt ggagctgaag gcgtatgaga agctggatga agagcacagg agactgagag
2521 aggcgtcggg agaaggctgg aaggggcagg atcctttcag ggacctgcac agcctcctga
2581 tggagatcca ggctctgcgc ttgcaactag aaaggagcat cgaaaccagc agcactctgc
2641 agagcaggct caaggaacag ctggcaaggg gggcagagaa ggcacaggaa ggagccctca
2701 ctctggctgt ccaagccgtg tccatccctg aggtgcccct tcagcctgac aaacacgatg
2761 gtgacaaata tcccatggaa agtgataatt catttgatct gtttgattcc tcccaggcag
2821 tgacaccaaa atcagtttca gagactcctc cactctctgg gaatgacacg gactccctct
2881 cctgcgacag tggcagttcg gcaactagca ctccgtgtgt gtcccgcctg gtcactggcc
2941 accacctgtg ggccagcaag aatggccgcc atgtcctggg cctgattgag gactatgagg
3001 ccctgctcaa acagatcagc cagggacaga ggctccttgc tgaaatggac attcaaaccc
3061 aagaggctcc cagctccaca agtcaagagc tgggaacaaa gggtccacac ccagcaccac
3121 tgagcaagtt tgtgagcagt gtgagcacgg ccaagctgac cctggaagag gcctacaggc
3181 ggctgaagct tctctggaga gtctcactcc ccgaggatgg ccagtgcccc cttcactgtg
3241 agcagattgg agaaatgaag gcagaggtca ccaaactaca taaaaaattg tttgaacaag
3301 aaaagaagtt gcaaaacacc atgaagcttt tgcagctgag caagcgccag gaaaaagtca
3361 tctttgatca attggtcgta acccacaaaa tccttcggaa ggccagagga aacctggagc
3421 ttaggcctgg gggagcccat ccaggaacat gcagtcccag cagaccaggc tcctgtacag
3481 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
3541 acaagtaact cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
3601 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
3661 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct
3721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
3781 gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt
3841 aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
3901 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
3961 agatgtggga ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc
4021 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
4081 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
4141 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
4201 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
4261 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
4321 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
4381 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
4441 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
4501 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
4561 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
4621 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
4681 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
4741 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
4801 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
4861 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
4921 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
4981 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
5041 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
5101 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
5161 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
5221 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
5281 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
5341 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
5401 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
5461 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
5521 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
5581 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
5641 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
5701 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
5761 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
5821 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
5881 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
5941 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
6001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
6061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
6121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
6181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
6241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
6301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
6361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
6421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
6481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
6541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
6601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
6661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
6721 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDK5RAP2-FLAG质粒载体应用举例
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