PCDH-CMV-mCherry-TRIM37

LOCUS       PCDH-CMV-mCherry-TRIM37              10044 bp DNA     circular SYN 21-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3220..3257
                     /label=TRIM37-F
     misc_feature    3240..6134
                     /label=TRIM37
     primer_bind     3568..3589
                     /label=TRIM37-seqF0
     primer_bind     complement(3926..3947)
                     /label=TRIM37-closeqR
     primer_bind     4268..4289
                     /label=TRIM37-seqF1
     primer_bind     5040..5059
                     /label=TRIM37-seqF2
     primer_bind     complement(6114..6152)
                     /label=TRIM37-R
     misc_feature    6141..6728
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6612..6623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6801..7034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7106..7227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7246..7381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7419..7435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7443..7459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7467..7497)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7512..7533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7821..8409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8580..9440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9441..9545)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9914..9927)
                     /label=Primer 5
     primer_bind     10019..10035
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggatgaaca gagcgtggag agcattgctg aggttttccg atgtttcatt tgtatggaga
     3301 aattgcggga tgcacgcctg tgtcctcatt gctccaaact gtgttgtttc agctgtatta
     3361 ggcgctggct gacagagcag agagctcaat gtcctcattg ccgtgctcca ctccagctac
     3421 gagaactagt aaattgtcgt tgggcagaag aagtaacaca acagcttgat actcttcaac
     3481 tctgcagtct caccaaacat gaagaaaatg aaaaggacaa atgtgaaaat caccatgaaa
     3541 aacttagtgt attttgctgg acttgtaaga agtgtatctg ccatcagtgt gcactttggg
     3601 gaggaatgca tggcggacat acctttaaac ctttggcaga aatttatgag caacacgtca
     3661 ctaaagtgaa tgaagaggta gccaaacttc gtcggcgtct catggaactg atcagcttag
     3721 ttcaagaagt ggaaaggaat gtagaagctg taagaaatgc aaaagatgag cgtgttcggg
     3781 aaattaggaa tgcagtggag atgatgattg cacggttaga cacacagctg aagaataagc
     3841 ttataacact gatgggtcag aagacatctc taacccaaga aacagagctt ttggaatcct
     3901 tacttcagga ggtggagcac cagttgcggt cttgtagtaa gagtgagttg atatctaaga
     3961 gctcagagat ccttatgatg tttcagcaag ttcatcggaa gcccatggca tcttttgtta
     4021 ccactcctgt tccaccagac tttaccagtg aattagtgcc atcttacgat tcagctactt
     4081 ttgttttaga gaatttcagc actttgcgtc agagagcaga tcctgtttac agtccacctc
     4141 ttcaagtttc aggactttgc tggaggttaa aagtttaccc agatggaaat ggagttgtgc
     4201 gaggttacta cttatctgtg tttctggagc tctcagctgg cttgcctgaa acttctaaat
     4261 atgaatatcg tgtagagatg gttcaccagt cctgtaatga tcctacaaaa aatatcattc
     4321 gagaatttgc atctgacttt gaagttggag aatgctgggg ctataataga tttttccgtt
     4381 tggacttact cgcaaatgaa ggatacttga atccacaaaa tgatacagtg attttaaggt
     4441 ttcaggtacg ttcaccaact ttctttcaaa aatcccggga ccagcattgg tacattactc
     4501 agttggaagc tgcacagact agttatatcc aacaaataaa caaccttaaa gagagactta
     4561 ctattgagct gtctcgaact cagaagtcaa gagatttgtc accaccagat aaccatctta
     4621 gcccccaaaa tgatgatgct ctggagacac gagctaagaa gtctgcatgc tctgacatgc
     4681 ttctcgaagg tggtcctact acagcttctg taagagaggc caaagaggat gaagaagatg
     4741 aggagaagat tcagaatgaa gattatcatc acgagctttc agatggagat ctggatctgg
     4801 atcttgttta tgaggatgaa gtaaatcagc tcgatggcag cagttcctct gctagttcca
     4861 cagcaacaag taatacagaa gaaaatgata ttgatgaaga aactatgtct ggagaaaatg
     4921 atgtggaata taacaacatg gaattagaag agggagaact catggaagat gcagctgctg
     4981 caggacccgc aggtagtagc catggttatg tgggttccag tagtagaata tcaagaagaa
     5041 cacatttatg ctccgctgct accagtagtt tactagacat tgatccatta attttaatac
     5101 atttgttgga ccttaaggac cggagcagta tagaaaattt gtggggctta cagcctcgcc
     5161 cacctgcttc acttctgcag cccacagcat catattctcg aaaagataaa gaccaaagga
     5221 agcaacaggc aatgtggcga gtgccctctg atttaaagat gctaaaaaga ctcaaaactc
     5281 aaatggccga agttcgatgt atgaaaactg atgtaaagaa tacactttca gaaataaaaa
     5341 gcagcagtgc tgcttctgga gacatgcaga caagcctttt ttctgctgac caggcagctc
     5401 tggctgcatg tggaactgaa aactctggca gattgcagga tttgggaatg gaactcctgg
     5461 caaagtcatc agttgccaat tgttacatac gaaactccac aaataagaag agtaattcgc
     5521 ccaagccagc tcgatccagt gtagcaggta gtctatcact tcgaagagca gtggaccctg
     5581 gagaaaatag tcgttcaaag ggagactgtc agactctgtc tgaaggctcc ccaggaagct
     5641 ctcagtctgg gagcaggcac agttctcccc gagccttgat acatggcagt atcggtgata
     5701 ttctgccaaa aactgaagac cggcagtgta aagctttgga ttcagatgct gttgtggttg
     5761 cagttttcag tggcttgcct gcggttgaga aaaggaggaa aatggtcacc ttgggggcta
     5821 atgctaaagg aggtcatctg gaaggactgc agatgactga tttggaaaat aattctgaaa
     5881 ctggagagtt acagcctgta ctacctgaag gagcttcagc tgcccctgaa gaaggaatga
     5941 gtagcgacag tgacattgaa tgtgacactg agaatgagga gcaggaagag cataccagtg
     6001 tgggcgggtt tcacgactcc ttcatggtca tgacacagcc cccggatgaa gatacacatt
     6061 ccagttttcc tgatggtgaa caaataggcc ctgaagatct cagcttcaat acagatgaaa
     6121 atagtggaag gtaaggatcc aatcaacctc tggattacaa aatttgtgaa agattgactg
     6181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6541 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6661 tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc
     6721 ctccccgccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6781 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     6841 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7021 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7081 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7141 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7201 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7261 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7321 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7381 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     7441 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7501 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7561 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7621 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7681 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7741 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7801 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7861 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7921 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7981 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8041 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8101 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8161 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8221 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8281 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8341 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8401 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8461 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8521 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8581 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8641 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8701 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8761 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8821 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8881 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8941 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9001 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9061 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9121 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9181 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9241 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9301 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9361 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9421 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9481 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9541 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     9601 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     9661 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     9721 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     9781 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     9841 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     9901 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     9961 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    10021 aaaacgacgg ccagtgccaa gctg
//