| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-EIF4G1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001631 |
| 产品用途: | 3' AAVLINK plasmid for EIF4G1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EIF4G1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-EIF4G1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-EIF4G1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-EIF4G1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-EIF4G1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-EIF4G1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-EIF4G1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-EIF4G1-FLAG 6452 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6452)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6452
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3165
/codon_start=1
/label=EIF4G1
/translation="VAAPLTPHDSPRSPSLAPSILTTSSLHLEGD*AGARAAAEAQEPS
PQTQHQKLLAQLLVL*IASQPFNKRYPQKAQIIDVWCRGVA*AENEARKLETEETA*SG
VNGEGTVGTGLIVRGHLLPSGASARKWRSGVENGPPSLRGCARQLASRRIGTVGGMP*S
EKLPYPQ*AP*RRLSLRRS*RRNPRLSLRNISISMT*KRQSSACRSWPHPPCSSSLYGM
VSSLRWSAVPLLVSIWGSCCTSCSVLGICLLLSTTKGCMKSWNWLRTWKLTSPTCGST*
RNW*HPFCRKVGCPWGSCSGRLQSL*DRWAKLLPCCWRSWASCAKAWVLKRWGRCGEKP
GLAGRNFYLKARTLVHSSLNRRWSIPWERSRKPLARGHSPPRS*TGSWRSC*RRAAVTS
GCSTG*RPT*VSSR*YPTR*FEPS*RLSAILQLFLRLPSEWTLQC*KRERSCCRNTCVT
SRRSYRRSTPSRPL**P*NSLPTCCGCSLTHCMTRTW*RRMPSTVGRVARTPLSSRARV
WPLNLSQPSSSGSVKQRRSLTT"
misc_feature 2084..2089
/label=SDS
misc_feature 2183..2188
/label=SDS
misc_feature 2511..2516
/label=SDS
misc_feature 2723..2728
/label=SDS
misc_feature 3035..3040
/label=SDS
misc_feature 3146..3151
/label=SDS
CDS 3172..3237
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3241..3672
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3385..3390
/label=SDS
repeat_region 3715..3855
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3715..3844
/label=AAV2 ITR
rep_origin 3930..4385
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4667..4771
/gene="bla"
/label=AmpR promoter
CDS 4772..5632
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5803..6391
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtag ccgccccatt
1561 gacacctcac gactcaccaa gatcaccaag cctggctcca tcgattctaa caaccagctc
1621 tttgcacctg gagggcgact gagctggggc aagggcagca gcggaggctc aggagccaag
1681 ccctcagacg cagcatcaga agctgctcgc ccagctacta gtactttgaa tcgcttctca
1741 gcccttcaac aagcggtacc cacagaaagc acagataata gacgtgtggt gcagaggagt
1801 agcttgagcc gagaacgagg cgagaaagct ggagaccgag gagaccgcct agagcggagt
1861 gaacggggag gggaccgtgg ggaccggctt gatcgtgcgc ggacacctgc taccaagcgg
1921 agcttcagca aggaagtgga ggagcggagt agagaacggc cctcccagcc tgaggggctg
1981 cgcaaggcag ctagcctcac ggaggatcgg gaccgtgggc gggatgccgt gaagcgagaa
2041 gctgccctac ccccagtgag ccccctgaag gcggctctct ctgaggagga gttagagaag
2101 aaatccaagg ctatcattga ggaatatctc catctcaatg acatgaaaga ggcagtccag
2161 tgcgtgcagg agctggcctc accctccttg ctcttcatct ttgtacggca tggtgtcgag
2221 tctacgctgg agcgcagtgc cattgctcgt gagcatatgg ggcagctgct gcaccagctg
2281 ctctgtgctg ggcatctgtc tactgctcag tactaccaag ggttgtatga aatcttggaa
2341 ttggctgagg acatggaaat tgacatcccc cacgtgtggc tctacctagc ggaactggta
2401 acacccattc tgcaggaagg tggggtgccc atgggggagc tgttcaggga gattacaaag
2461 cctctgagac cgttgggcaa agctgcttcc ctgttgctgg agatcctggg cctcctgtgc
2521 aaaagcatgg gtcctaaaaa ggtggggacg ctgtggcgag aagccgggct tagctggaag
2581 gaatttctac ctgaaggcca ggacattggt gcattcgtcg ctgaacagaa ggtggagtat
2641 accctgggag aggagtcgga agcccctggc cagagggcac tcccctccga ggagctgaac
2701 aggcagctgg agaagctgct gaaggagggc agcagtaacc agcgggtgtt cgactggata
2761 gaggccaacc tgagtgagca gcagatagta tccaacacgt tagttcgagc cctcatgacg
2821 gctgtctgct attctgcaat tatttttgag actcccctcc gagtggacgt tgcagtgctg
2881 aaagcgcgag cgaagctgct gcagaaatac ctgtgtgacg agcagaagga gctacaggcg
2941 ctctacgccc tccaggccct tgtagtgacc ttagaacagc ctcccaacct gctgcggatg
3001 ttctttgacg cactgtatga cgaggacgtg gtgaaggagg atgccttcta cagttgggag
3061 agtagcaagg accccgctga gcagcagggc aagggtgtgg cccttaaatc tgtcacagcc
3121 ttcttcaagt ggctccgtga agcagaggag gagtctgacc acaactgtac agactacaaa
3181 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa
3241 ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
3301 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
3361 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg
3421 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
3481 acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct
3541 agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
3601 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
3661 gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
3721 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
3781 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
3841 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
3901 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
3961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
4021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
4081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
4141 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
4201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
4261 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
4321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
4381 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
4441 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
4501 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
4561 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
4621 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
4681 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
4741 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
4801 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
4861 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
4921 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
4981 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
5041 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
5101 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
5161 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
5221 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
5281 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
5341 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
5401 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
5461 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
5521 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
5581 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
5641 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
5701 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
5761 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
5821 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
5881 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
5941 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
6001 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
6061 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
6121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
6181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
6241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
6301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
6361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
6421 cctggccttt tgctggcctt ttgctcacat gt
//
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