pU6-EMX1-PegRNA

LOCUS       pU6-EMX1-PegRNA         2337 bp DNA     circular SYN 12-MAR-2026
DEFINITION  Prime editing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  TITLE     Liu Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          250..269
                     /chromosome="2"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /organism="Homo sapiens"
     source          346..380
                     /chromosome="2"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /organism="Homo sapiens"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     247..272
                     /label=OL2774
     misc_feature    250..269
                     /label=EMX1 spacer
     misc_RNA        270..345
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    346..367
                     /label=RTT
     primer_bind     complement(360..380)
                     /label=OL2775
     misc_feature    368..380
                     /label=PBS
     rep_origin      complement(507..1095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(587..606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1266..2126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1889..1908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2127..2231)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg agtccgagca gaagaagaag ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcgttga tgtgatggga
      361 gccgttcttc ttctgctcgg gtcggcatgg ccccattcgc acgttttttt aagcttgggc
      421 cgctcgaggt acctctctac atatgacatg tgagcaaaag gccagcaaaa ggccaggaac
      481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      961 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1021 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1081 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1141 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1201 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1261 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1381 ggccccagtg ctgcaatgat accgcgagat ccacgctcac cggctccaga tttatcagca
     1441 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1501 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1561 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2221 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgctagctg tacaaaaaag
     2281 caggctttaa aggaaccaat tcagtcgact ggatccggta ccaaggtcgg gcaggaa
//