| 质粒名称: | pQL48 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002499 |
| 产品用途: | Synthetic mammalian ETS TF-dependent SEAP expression vector ((GGAA)7-PhCMVmin-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | Synthetic |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
pQL48
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pQL48载体质粒基本信息
pQL48质粒图谱载体图谱和pQL48载体序列质粒序列多克隆位点信息
pQL48质粒载体简介
pQL48质粒序列载体序列
LOCUS pQL48 6464 bp DNA circular SYN 02-MAR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 6464)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6464
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 213..241
/label=QL94 GA-F
primer_bind 225..269
/label=QL70 GA-F
misc_feature 234..269
/label=GGAA
primer_bind complement(249..291)
/label=QL71 GA-R
primer_bind complement(272..312)
/label=QL95 GA-R
primer_bind 292..314
/label=Oscl23-1
misc_feature 298..445
/label=hcmvmin
primer_bind complement(430..445)
/label=oYW11
misc_feature 468..491
/label=New Feature
primer_bind 492..518
/label=Oscl13-1
misc_feature 493..2013
/label=SEAP
primer_bind complement(1993..2021)
/label=Oscl23-2
primer_bind complement(1995..2013)
/label=Oscl13-2
terminator 2061..2288
/label=bGH PA
misc_feature 2956..3033
/label=SV40 ORI
CDS 3175..3966
/label=Kan/neoR
primer_bind complement(4306..4326)
/label=M13-rev
misc_binding complement(4332..4354)
/label=LacO
promoter complement(4359..4388)
/label=lac
CDS complement(5471..6130)
/label=AmpR
promoter complement(6370..6398)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgtacgga
241 aggaaggaag gaaggaagga aggaagatcc tagactcggc ggcctactta ggctagctcg
301 agctcggtac ccgggtcgag taggcgtgta cggtgggagg cctatataag cagagctcgt
361 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
421 caccgggacc gatccagcct ccgcggcccc gaattcgagc tcgcccgggg atcctctaga
481 gtcagcttct gcatgctgct gctgctgctg ctgctgggcc tgaggctaca gctctccctg
541 ggcatcatcc cagttgagga ggagaacccg gacttctgga accgcgaggc agccgaggcc
601 ctgggtgccg ccaagaagct gcagcctgca cagacagccg ccaagaacct catcatcttc
661 ctgggcgatg ggatgggggt gtctacggtg acagctgcca ggatcctaaa agggcagaag
721 aaggacaaac tggggcctga gatacccctg gccatggacc gcttcccata tgtggctctg
781 tccaagacat acaatgtaga caaacatgtg ccagacagtg gagccacagc cacggcctac
841 ctgtgcgggg tcaagggcaa cttccagacc attggcttga gtgcagccgc ccgctttaac
901 cagtgcaaca cgacacgcgg caacgaggtc atctccgtga tgaatcgggc caagaaagca
961 gggaagtcag tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc agccggcacc
1021 tacgcccaca cggtgaaccg caactggtac tcggacgccg acgtgcctgc ctcggcccgc
1081 caggaggggt gccaggacat cgctacgcag ctcatctcca acatggacat tgacgtgatc
1141 ctaggtggag gccgaaagta catgtttccc atgggaaccc cagaccctga gtacccagat
1201 gactacagcc aaggtgggac caggctggac gggaagaatc tggtgcagga atggctggcg
1261 aagcgccagg gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc ttccctggac
1321 ccgtctgtga cccatctcat gggtctcttt gagcctggag acatgaaata cgagatccac
1381 cgagactcca cactggaccc ctccctgatg gagatgacag aggctgccct gcgcctgctg
1441 agcaggaacc cccgcggctt cttcctcttc gtggagggtg gtcgcatcga ccatggtcat
1501 catgaaagca gggcttaccg ggcactgact gagacgatca tgttcgacga cgccattgag
1561 agggcgggcc agctcaccag cgaggaggac acgctgagcc tcgtcactgc cgaccactcc
1621 cacgtcttct ccttcggagg ctaccccctg cgagggagct ccatcttcgg gctggcccct
1681 ggcaaggccc gggacaggaa ggcctacacg gtcctcctat acggaaacgg tccaggctat
1741 gtgctcaagg acggcgcccg gccggatgtt accgagagcg agagcgggag ccccgagtat
1801 cggcagcagt cagcagtgcc cctggacgaa gagacccacg caggcgagga cgtggcggtg
1861 ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc aggagcagac cttcatagcg
1921 cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg cctgcgacct ggcgcccccc
1981 gccggcacca ccgacgccgc gcacccgggt taagcggccg ctcgagtcta gagggcccgt
2041 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
2101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
2161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
2221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
2281 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
2341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
2401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
2461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
2521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
2581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
2641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
2701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
2761 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
2821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
2881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
2941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
3061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
3121 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
3181 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
3241 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
3301 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
3361 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
3421 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
3481 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
3541 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
3601 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
3661 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
3721 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
3781 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
3841 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
3901 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
3961 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
4021 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
4081 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
4141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
4201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
4261 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
4321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
4381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
4441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
4501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
4561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
4621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
4681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
4741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
4801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
4861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
4921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
4981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
5101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
5161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
5221 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
5281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
5341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
5401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
5461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
5521 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
5581 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
5641 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
5701 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
5761 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
5821 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
5881 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
5941 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
6001 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
6061 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
6121 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
6181 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
6241 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
6301 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
6361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
6421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
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