| 质粒名称: | pSL1519 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002447 |
| 产品用途: | AAV2-vector for light-inducible MCP/LIRP-dependent EGFP translation (ITR(AAV)-PhCMV-(MS2)8-EGFP-pA: PhCMV-LIRP-pA-ITR(AAV)). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pX601
|
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV, PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Stbl3
|
|
插入基因信息 |
|
| 插入基因名称: | EGFP, LIRP |
| 物种来源: | synthetic |
| 密码子优化: | 人源表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1519
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1519载体质粒基本信息
pSL1519质粒图谱载体图谱和pSL1519载体序列质粒序列多克隆位点信息
pSL1519质粒载体简介
pSL1519质粒序列载体序列
LOCUS pSL1519 6254 bp DNA circular SYN 28-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6254)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6254
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
primer_bind 123..164
/label=oSCL1800
misc_feature 143..725
/label=CMV
primer_bind complement(699..730)
/label=OXB018
primer_bind 706..749
/label=oSCL1791
primer_bind 709..729
/label=oSCL1013
misc_feature 731..732
/label=linker
primer_bind complement(734..748)
/label=Oscl28-2
primer_bind complement(735..748)
/label=8x R
misc_feature 749..761
/label=linker
misc_feature 767..785
/label=MS2 box
misc_feature 791..800
/label=linker
misc_feature 806..824
/label=MS2 box
misc_feature 830..839
/label=linker
misc_feature 830..839
/label=linker
misc_feature 838..868
/label=hairpin
misc_feature 844..862
/label=MS2 box
misc_feature 867..879
/label=linker
misc_feature 878..908
/label=hairpin
misc_feature 884..902
/label=MS2 box
misc_feature 912..917
/label=linker
primer_bind 925..943
/label=oSCL2107
misc_feature 926..941
/label=linker
misc_feature 968..980
/label=linker
misc_feature 986..1004
/label=MS2 box
misc_feature 1010..1019
/label=linker
misc_feature 1025..1043
/label=MS2 box
misc_feature 1049..1058
/label=linker
misc_feature 1049..1058
/label=linker
misc_feature 1057..1087
/label=hairpin
misc_feature 1063..1081
/label=MS2 box
misc_feature 1086..1098
/label=linker
misc_feature 1097..1127
/label=hairpin
misc_feature 1103..1121
/label=MS2 box
misc_feature 1131..1136
/label=linker
primer_bind 1144..1192
/label=oSCL2107
misc_feature 1145..1160
/label=linker
misc_feature 1166..1900
/label=EGFP
/note="note=""Fragment extracted from OWS233-Kpn1-EGFP-Xba1
[758 nt] : (KpnI[4] / XbaI[766])"
primer_bind 1175..1192
/label=Oscl9-1
/note="for primer of EGFP"
CDS 1184..1897
/label=EGFP
primer_bind complement(1700..1711)
/label=oSCL048
primer_bind complement(1879..1920)
/label=oSCL2108
primer_bind complement(1879..1900)
/label=Oscl9-2
primer_bind 1887..1935
/label=oSCL2338
primer_bind 1900..1935
/label=oSCL1795
primer_bind complement(1900..1920)
/label=oSCL1849
polyA_signal 1907..2028
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 1907..1935
/label=OXB007
primer_bind complement(1918..1937)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1931..1951
/label=PSG5-R(SV40-R)
primer_bind 1972..1991
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(1999..2028)
/label=OXB016
primer_bind complement(2003..2028)
/label=OXB008
primer_bind complement(2012..2034)
/label=oSCL1801
primer_bind complement(2012..2028)
/label=oSCL1174
primer_bind complement(2012..2028)
/label=oSCL1796
misc_feature 2033..2620
/label=CMV
primer_bind 2033..2054
/label=oSCL1800
primer_bind complement(2589..2620)
/label=OXB018
primer_bind 2596..2620
/label=oSCL1791
primer_bind 2599..2619
/label=oSCL1013
primer_bind 2621..2640
/label=T7
primer_bind 2627..2676
/label=oSCL2413
primer_bind 2650..2676
/label=oSCL2381
CDS 2659..2811
/codon_start=1
/label=MS2-N55K
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
CSVRQS"
CDS 2812..3222
/codon_start=1
/label=LOV2
/translation="LERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRF
LQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIG
VQLDGTEHVRDAAEREGVMLIKKTAENIDEAAG"
primer_bind 2816..2858
/label=oSCL2408
primer_bind complement(2816..2835)
/label=oSCL2407
CDS 2836..2838
/codon_start=1
/label=V416I
/translation="V"
primer_bind 3152..3194
/label=oSCL2410
primer_bind complement(3152..3171)
/label=oSCL2409
CDS 3172..3174
/codon_start=1
/label=G528A
/translation="G"
primer_bind 3182..3225
/label=oSCL2412
primer_bind complement(3182..3201)
/label=oSCL2411
CDS 3202..3204
/codon_start=1
/label=N538E
/translation="N"
CDS 3223..3450
/codon_start=1
/label=MS2-N55K
/translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind complement(3431..3473)
/label=oSCL2415
primer_bind complement(3431..3453)
/label=oSCL2383
primer_bind complement(3431..3450)
/label=oSCL2382
primer_bind 3431..3450
/label=oSCL2384
misc_feature 3460..3508
/label=HSV TK poly(A) signal
primer_bind complement(3484..3516)
/label=oSCL1799
repeat_region 3517..3657
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3517..3646
/label=AAV2 ITR
rep_origin 3732..4187
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3819..3838)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4029..4050
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4204..4223)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4323..4345
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4383..4401)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4469..4573
/gene="bla"
/label=AmpR promoter
CDS 4574..5434
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4792..4811)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5605..6193
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6094..6113
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gagttgacat tgattattga ctagttatta atagtaatca
181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
721 agcagagctc cttgaaccgg taagaattgc aacaaacaaa tcaccaccgt gaggatcacc
781 cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc
841 tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac
901 ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac tgatcggaga cgagtccgtc
961 tcaaattgca acaaacaaat caccaccgtg aggatcaccc acggtggtga aagccttagc
1021 ctagccgtga ggatcaccca cggtaggcac aagaaattct acccgtgagg atcacccacg
1081 ggtagaatca caaagactac agccgtgagg atcacccacg gctgtagact caaacttaaa
1141 aaggatatct tcagcaaact gaattcagct tagcgccacc atggtgagca agggcgagga
1201 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
1261 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
1321 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
1381 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
1441 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
1501 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
1561 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
1621 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
1681 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
1741 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
1801 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
1861 cgccgggatc actctcggca tggacgagct gtacaagtaa ctcgagtaag atacattgat
1921 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
1981 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttac gcgttgacat
2041 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
2101 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
2161 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
2221 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
2281 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
2341 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
2401 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
2461 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
2521 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
2581 ggtaggcgtg tacggtggga ggtctatata agcagagctc taatacgact cactataggg
2641 aagctttaag ccaccatggc tagcaacttc acacagttcg tgctggtgga caacggcggc
2701 accggcgacg tgaccgtggc ccctagcaac ttcgccaacg gcatcgccga gtggatcagc
2761 agcaacagca gaagccaagc ctacaaggtg acctgcagcg tgagacagag cctggagaga
2821 atcgagaaga acttcgtgat caccgaccct agactgcccg acaaccccat catcttcgct
2881 agcgacagct tcctgcagct gaccgagtac agcagagagg agatcctggg cagaaactgc
2941 agattcctgc aaggccccga gaccgacaga gccaccgtga gaaagatcag agacgccatc
3001 gacaatcaga ccgaggtgac cgtgcagctg atcaactaca ccaagagcgg caagaagttc
3061 tggaacctgt tccacctgca gcccatgaga gatcagaagg gcgacgtgca gtacttcatc
3121 ggcgtgcagc tggacggcac cgagcacgtg agagacgccg ccgagagaga gggcgtgatg
3181 ctgatcaaga agaccgccga gaacatcgac gaggccgccg ggcagaagag aaagtacacc
3241 atcaaagtcg aggtgcccaa ggtggccacc caaaccgtgg gcggcgtgga gctgcccgtg
3301 gccgcctgga gaagctacct gaacatggag ctgaccatcc ccatcttcgc caccaacagc
3361 gactgcgagc tgatcgtgaa ggccatgcaa ggcctgctga aggacggcaa ccccattcct
3421 agcgccatcg ctgctaacag cggcatttat taacaattgc ggcaataaaa agacagaata
3481 aaacgcacgg gtgttgggtc gtttgttcgc ggccgcagga acccctagtg atggagttgg
3541 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
3601 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
3661 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
3721 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
3781 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
3841 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
3901 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
3961 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4021 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
4081 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
4141 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4201 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4261 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4321 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
4381 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
4441 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4501 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4561 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
4621 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
4681 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
4741 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
4801 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
4861 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
4921 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
4981 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5041 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5101 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5161 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5221 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5281 tgagcgtgga agccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5341 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
5401 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
5461 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5521 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
5581 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5641 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
5701 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5761 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5821 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
5881 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
5941 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6001 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6061 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6121 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6181 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6241 ttttgctcac atgt
//
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