pCMV-Flag-GTBP1

LOCUS       pCMV-Flag-GTBP1             5427 bp DNA     circular SYN 04-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      607..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..615
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=initiation coden
                     /note="mammalian codon-optimized"
                     /translation="M"
     CDS             616..639
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    646..684
                     /label=Primer F
     CDS             664..2085
                     /codon_start=1
                     /label=GTBP
                     /translation="MQITSALTQNKMDPINGNLTSTAPVEPLSLTAAEQVGGAFCHQFY
                     ITVSENRKAITKFYGHESKFYLDDQVVTGSQEIVKLYNHLPETTHFKIQSIKGYPTPHK
                     QGVIINVIGTVNLRPFLQSFLLGQQGQKKYYVESDAFQYIDKFFVANTEKEKTTSSTGH
                     NGHASGANGKDSKSSKTEQPKEQSKPAPPKQQTPKKAEDRIVKQDTPKSVEKEVAKTPQ
                     KPQLAQQPPQQRAPVPEPVQEQPKVQKTWANLVGGGGKSQPPQQQSYGQGQQIHQQTQM
                     HHMQPSMHQQQQGSHAPLYQQQRQSHPANELRQAQESSDANLNERKIYLGGITREIVPS
                     NTAQAESEIKQVFYKYGEIEEVRMPRKVLDSPNDTSKHAFAFITMRNKAGVKAIFEAAT
                     KDEQGVWKLPLKLDAFGFDGHAQISEQRDQPQGGGFRGGRGGFQPRGGQFFRRGGSGGA
                     PRGGMRSGFQNAGQN"
     misc_feature    complement(2065..2103)
                     
     polyA_signal    2215..2336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2343..2798)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2825..2929
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2931..3288
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3139..3274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3323..4117
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4349..4396
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4725..5313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggatta caaggacgac gatgacaagt actcagatct cgagctcaag
      661 cttatgcaga taacctcggc cttaacccag aataagatgg atccgatcaa tggtaatttg
      721 acttccaccg ctccggtcga gccactctca ttgacagccg ctgaacaagt cggtggagcg
      781 ttctgccatc agttctatat caccgtctct gagaaccgta aagcgattac caagttctat
      841 ggacatgaat cgaagttcta tttggatgat caggtggtca ctggatcgca ggagattgtt
      901 aagttgtaca atcatctacc agaaaccacc cacttcaaga ttcaatctat caagggatac
      961 ccaaccccac acaaacaggg agtgattatc aatgttatcg gaaccgttaa cctccgtcca
     1021 ttccttcaat catttttgct tgggcaacaa gggcagaaaa aatactatgt cgaatcagac
     1081 gcttttcagt acattgataa gtttttcgtc gcgaatacgg aaaaggagaa gactacttcg
     1141 tcgactggtc ataacggaca cgccagtgga gcaaatggaa aggattccaa atcttccaaa
     1201 accgagcaac ctaaggaaca atcaaagccg gctcctccaa agcagcaaac gccaaagaaa
     1261 gctgaagatc gtatagtgaa gcaagacact ccgaaatctg tggaaaagga ggtagcgaaa
     1321 acaccgcaga aaccacagct tgcccaacag cccccacagc agagagcccc agttcctgag
     1381 cctgttcaag aacagccaaa ggttcagaaa acgtgggcta atcttgtggg tggaggtgga
     1441 aagtctcagc ccccacaaca acaaagttat ggacaaggac agcaaatcca tcagcaaaca
     1501 caaatgcacc acatgcagcc ttccatgcat cagcaacaac aaggatcgca tgctcctctt
     1561 tatcagcaac agagacagtc gcacccagct aatgaattgc gccaagctca ggaaagttca
     1621 gatgccaacc taaatgagcg taagatctat ttgggaggaa tcactcgaga gattgtcccc
     1681 agcaacacag ctcaggctga atcggaaatc aagcaggtgt tctacaaata tggagaaatc
     1741 gaggaggttc gcatgccgcg caaggttctt gacagtccga acgacacatc gaaacatgcc
     1801 tttgcattta ttaccatgcg taacaaggcc ggagtaaaag ccattttcga agcggctaca
     1861 aaggatgagc aaggcgtgtg gaagcttcca ctcaaactgg atgcattcgg atttgacgga
     1921 catgctcaaa ttagtgagca acgtgatcaa ccacaaggcg gcggattccg aggaggacgt
     1981 ggaggtttcc aaccacgcgg tggacaattc ttccgccgtg gcggttcggg tggtgctcca
     2041 cgaggtggta tgcgcagcgg tttccaaaat gcgggacaaa attagggatc caccggatct
     2101 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2161 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2221 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2281 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     2341 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2401 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2461 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2521 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     2581 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     2641 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     2701 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     2761 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     2821 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2881 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     2941 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3001 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3061 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3121 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3241 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     3301 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3361 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3421 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3481 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     3541 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3601 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     3661 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     3721 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     3781 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     3841 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     3901 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     3961 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4021 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4081 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     4141 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     4201 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4261 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     4321 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     4381 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4441 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4501 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4561 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4621 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4681 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4741 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4801 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4861 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4921 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4981 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5041 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5101 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5161 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5221 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5281 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5341 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5401 gtggataacc gtattaccgc catgcat
//