| 质粒名称: | pDG212 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002911 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for LID-tagged NanoLuc (PhCMV-LID-NanoLuc-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | LID, NanoLuc |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pDG212
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDG212载体质粒基本信息
pDG212质粒图谱载体图谱和pDG212载体序列质粒序列多克隆位点信息
pDG212质粒载体简介
pDG212质粒序列载体序列
LOCUS pDG212 6419 bp DNA circular SYN 21-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6419)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6419
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind complement(883..922)
/label=Primer 1
primer_bind 887..930
/label=oSCL1108
primer_bind 898..930
/label=oSCL1314
primer_bind 898..922
/label=Primer 1a
primer_bind 898..922
/label=Primer 7
primer_bind 898..922
/label=Primer 9
primer_bind 898..922
/label=Primer 14
primer_bind 898..922
/label=Primer 16
primer_bind 898..922
/label=Primer 19
primer_bind 898..922
/label=Fragment.REV
primer_bind 903..930
/label=oSCL1846
primer_bind 903..926
/label=oSCL1848
primer_bind 903..922
/label=Primer 3
primer_bind 903..922
/label=Primer 22
primer_bind 907..930
/label=oSCL084
misc_feature 909..977
/label=Secretory (IgK)
/label=nonstandard type: signal
misc_feature 909..911
/label=SEAP
primer_bind complement(976..995)
/label=Primer 8
primer_bind 994..1015
/label=Primer 17
misc_feature 999..1508
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
primer_bind 999..1018
/label=Primer 5
misc_feature 1029..1031
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1488..1508)
/label=oSCL1849
primer_bind 1489..1529
/label=Primer 2
primer_bind complement(1489..1522)
/label=Primer 15
primer_bind complement(1489..1509)
/label=Primer 13
primer_bind complement(1489..1508)
/label=Primer 4
primer_bind complement(1489..1508)
/label=Primer 6
primer_bind complement(1489..1508)
/label=Primer 10
primer_bind complement(1489..1508)
/label=Primer 18
primer_bind complement(1489..1508)
/label=Primer 20
primer_bind complement(1498..1518)
/label=oSCL085
primer_bind complement(1498..1517)
/label=oSCL1109
primer_bind 1509..1556
/label=oSCL1217
CDS 1509..1538
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
primer_bind 1509..1538
/label=oSCL388
primer_bind 1512..1533
/label=Primer 5
primer_bind complement(1516..1534)
/label=Oscl18-4
misc_feature 1539..1859
/label=ILD
primer_bind complement(1625..1666)
/label=oSCL1214
primer_bind 1647..1666
/label=oSCL1215
primer_bind complement(1839..1897)
/label=oSCL1218
primer_bind complement(1839..1858)
/label=Oscl18-3
primer_bind complement(1841..1867)
/label=Oscl18-2
misc_feature 1861..1867
/label=linker
primer_bind complement(1878..1936)
/label=oSCL1219
primer_bind complement(1943..1960)
/label=Primer 18
primer_bind complement(1943..1959)
/label=Primer 6
primer_bind complement(1990..2008)
/label=oYW306
terminator 2016..2243
/label=bGH PA
misc_feature 2911..2988
/label=SV40 ORI
primer_bind 3046..3065
/label=Primer 1a
CDS 3130..3921
/label=Kan/neoR
primer_bind complement(4261..4281)
/label=M13-rev
misc_binding complement(4287..4309)
/label=LacO
promoter complement(4314..4343)
/label=lac
CDS complement(5426..6085)
/label=AmpR
promoter complement(6325..6353)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtgccaccat gactagtgag acagacacac tcctgctatg ggtactgctg ctctgggttc
961 caggttccac tggtgacgct agtggtggtt ctggtatggt cttcacactc gaagatttcg
1021 ttggggactg gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg
1081 tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga
1141 gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
1201 gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc
1261 actttaaggt gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga
1321 tcgactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
1381 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg
1441 gctccctgct gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca
1501 ttctggcggc cagcggaagt ggcggaggag gcgacgtcgg cgttcaggtt gaaaccatta
1561 gcccgggaga tggccgtaca tttccgaaac gtggccagac atgtgttgtt cactataccg
1621 gcatgctgga ggatggtaaa aaagtggata gcagccgtga ccgtaataaa ccgttcaaat
1681 tcatgctggg caaacaggaa gtcattcgcg gttgggaaga aggtgtcgca cagatgagtg
1741 tgggtcaacg tgccaaactg acgattagtc ctgactatgc ctatggagca acaggtcatc
1801 cagggattat tccgcctcac gctactctgg tctttgatgt ggaactgctg aaactggaaa
1861 ccaggggcgt ggaggaggtt gctgaaggag tggtgctgct gaggaggagg ggcaactaag
1921 gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga
1981 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
2041 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
2101 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
2161 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
2221 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
2281 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
2341 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
2401 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
2461 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
2521 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
2581 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
2641 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
2701 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
2761 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
2821 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
2881 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
2941 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
3001 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
3061 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
3121 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
3181 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
3241 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
3301 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
3361 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
3421 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
3481 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
3541 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
3601 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
3661 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
3721 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
3781 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
3841 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
3901 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
3961 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
4021 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
4081 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
4141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
4201 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
4261 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
4321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
4381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
4441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
4501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
5041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
5101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
5161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
5221 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
5281 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
5341 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
5401 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
5461 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
5521 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
5581 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
5641 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
5701 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
5761 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
5821 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
5881 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
5941 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
6001 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
6061 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
6121 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
6181 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
6241 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
6301 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
6361 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
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