pGA-01-47

LOCUS       pGA-01-47       2701 bp    DNA     circular SYN 04-SEP-2026
DEFINITION  MagLOV2 for constitutive expression in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abrahams G, Stuhec A, Spreng V, Henry R, Kempf I, James J, Sechkar 
            K, Stacey S, Trelles-Fernandez V, Antill LM, Timmel CR, Miller JJ, 
            Ingaramo M, York AG, Tetienne JP, Steel H
  TITLE     Quantum spin resonance in engineered proteins for multimodal 
            sensing.
  JOURNAL   Nature. 2026 Jan 21. doi: 10.1038/s41586-025-09971-3.
  PUBMED    41565820
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 4, 2026 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2026 Jan 21. doi: 10.1038/s41586-025-09971-3."
COMMENT     Sequence Label: pGA-01-47
FEATURES             Location/Qualifiers
     source          1..2701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(793..820)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1279..1296)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1314..1322
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(1359..1393)
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     misc_feature    1411..1430
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      1433..1476
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1480..1498)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1566..1670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1671..1739,1740..2531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1889..1908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcctgca gcgtctctcc
      781 ggaacatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatcg attacaactc
      841 ttttgccgct tccataatgt tttcggcggt ctttttaatt aacatgacgc gttcacggcc
      901 ggccgcatct cgaacatgtt cggtaccatc cagcttaact ccaatgaagt actgtacatc
      961 acctttttga tcacgcatcg gctgcacgtg caggagattc caaaactttt tgccactttt
     1021 ggtataattt attaactgga ctgtcacctc ggtctggttg tcgatcgcat cgcggatttt
     1081 gcgcacagtg gcacggtccg tttctgggcc ttgcagaaac cggggattcc accctagaat
     1141 ttcttcgcgg ctatactccg tcaactgaag gaatgagtcc gatgcaaaaa taatcgggtt
     1201 atcaggcagg cgcggatccg tgatcacgaa gtttttctcg atacgttcca gggtagtagc
     1261 cagcatgcta gccataccat gatgatgatg atgatgagaa ccccgcatat tatacctcct
     1321 tattgatttt ccaaaaccta actagtcctt gactagttgc tagcattata cctaggactg
     1381 agctagctgt cagatagggc atgagacgtt ctagaagcgg ccgcgaattc cagaaatcat
     1441 ccttagcgaa agctaaggat tttttttatc tgaaattctg cctcgtgata cgcctatttt
     1501 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1561 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1621 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1681 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1741 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1801 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1861 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1921 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1981 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2041 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2101 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2161 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2221 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2281 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2341 cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct cgcggtatca
     2401 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2461 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2521 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2581 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2641 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2701 c
//