| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL3A1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001655 |
| 产品用途: | 3' AAVLINK plasmid for COL3A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL3A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL3A1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL3A1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL3A1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL3A1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL3A1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL3A1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL3A1-FLAG 6731 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6731)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6731
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3444
/codon_start=1
/label=COL3A1
/translation="VKEALLELQDPLEVLDLLVLLVPKVSKVNVAVLVDLVLLASLVLV
VFLVLLVVMVTQDPQVPAVLQARMGPQVLRVTLVLLAALECLDQKVMLANQERRDRLVP
RAHQELQAHLGLLGSLEHGVLQDHQACQVLGEALALRVSRVKVGNQELTVSVENVVPLD
PRVFLVWLVQLVNLEEMETLDQMVFQAEMDLLVARVIVVKMALLVPLALLVIQAHLVLS
VQLERVVTEEKVALLALLVLPVLLVPEVLLVLKAHVVTKVKQVNVELLASKDIEDSLVI
QVPQVLQALLVSRVQSAVQDLQAPEDLLDPVDLLAKMEPVDIQVPLDHQGLEVTEVKED
LRAPQATQGNQALLDLLVPLVLAVVVLEPLPLLGLEVKKLAVLPRIMEMNQWISKSTPM
RL*LHSSLLMDK*KASLVLMVLVKTPLETAET*NSAILNSRVENTGLTLTKDANWMLSR
YSVIWKLGKHA*VPIL*MFHGNTGGQILVLRRNTFGLESPWMVVFSLATAILNFLKMSL
MCIWHSFDFSPAELPRTSHITAKIALHTWIRPVEM*RRP*S*WGQMKVNSRLKEIANSP
TQFWRMVARNTLGNGAKQSLNIEHARL*DYLL*ILHPMTLVVLIKNLVWTLALFAF"
misc_feature 1551..1556
/label=SDS
misc_feature 1558..1563
/label=SDS
misc_feature 1603..1608
/label=SDS
misc_feature 1693..1698
/label=SDS
misc_feature 2485..2490
/label=SDS
misc_feature 2602..2607
/label=SDS
misc_feature 2611..2616
/label=SDS
CDS 3451..3516
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3520..3951
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3664..3669
/label=SDS
repeat_region 3994..4134
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3994..4123
/label=AAV2 ITR
rep_origin 4209..4664
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4946..5050
/gene="bla"
/label=AmpR promoter
CDS 5051..5911
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6082..6670
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga aggaggccct
1561 cctggagttg caggaccccc tggaggttct ggacctgctg gtcctcctgg tccccaaggt
1621 gtcaaaggtg aacgtggcag tcctggtgga cctggtgctg ctggcttccc tggtgctcgt
1681 ggtcttcctg gtcctcctgg tagtaatggt aacccaggac ccccaggtcc cagcggttct
1741 ccaggcaagg atgggccccc aggtcctgcg ggtaacactg gtgctcctgg cagccctgga
1801 gtgtctggac caaaaggtga tgctggccaa ccaggagaga agggatcgcc tggtgcccag
1861 ggcccaccag gagctccagg cccacttggg attgctggga tcactggagc acggggtctt
1921 gcaggaccac caggcatgcc aggtcctagg ggaagccctg gccctcaggg tgtcaagggt
1981 gaaagtggga aaccaggagc taacggtctc agtggagaac gtggtccccc tggaccccag
2041 ggtcttcctg gtctggctgg tacagctggt gaacctggaa gagatggaaa ccctggatca
2101 gatggtcttc caggccgaga tggatctcct ggtggcaagg gtgatcgtgg tgaaaatggc
2161 tctcctggtg cccctggcgc tcctggtcat ccaggcccac ctggtcctgt cggtccagct
2221 ggaaagagtg gtgacagagg agaaagtggc cctgctggcc ctgctggtgc tcccggtcct
2281 gctggttccc gaggtgctcc tggtcctcaa ggcccacgtg gtgacaaagg tgaaacaggt
2341 gaacgtggag ctgctggcat caaaggacat cgaggattcc ctggtaatcc aggtgcccca
2401 ggttctccag gccctgctgg tcagcagggt gcaatcggca gtccaggacc tgcaggcccc
2461 agaggacctg ttggacccag tggacctcct ggcaaagatg gaaccagtgg acatccaggt
2521 cccattggac caccagggcc tcgaggtaac agaggtgaaa gaggatctga gggctcccca
2581 ggccacccag ggcaaccagg ccctcctgga cctcctggtg cccctggtcc ttgctgtggt
2641 ggtgttggag ccgctgccat tgctgggatt ggaggtgaaa aagctggcgg ttttgccccg
2701 tattatggag atgaaccaat ggatttcaaa atcaacaccg atgagattat gacttcactc
2761 aagtctgtta atggacaaat agaaagcctc attagtcctg atggttctcg taaaaacccc
2821 gctagaaact gcagagacct gaaattctgc catcctgaac tcaagagtgg agaatactgg
2881 gttgacccta accaaggatg caaattggat gctatcaagg tattctgtaa tatggaaact
2941 ggggaaacat gcataagtgc caatcctttg aatgttccac ggaaacactg gtggacagat
3001 tctagtgctg agaagaaaca cgtttggttt ggagagtcca tggatggtgg ttttcagttt
3061 agctacggca atcctgaact tcctgaagat gtccttgatg tgcatctggc attccttcga
3121 cttctctcca gccgagcttc ccagaacatc acatatcact gcaaaaatag cattgcatac
3181 atggatcagg ccagtggaaa tgtaaagaag gccctgaagc tgatggggtc aaatgaaggt
3241 gaattcaagg ctgaaggaaa tagcaaattc acctacacag ttctggagga tggttgcacg
3301 aaacacactg gggaatggag caaaacagtc tttgaatatc gaacacgcaa ggctgtgaga
3361 ctacctattg tagatattgc accctatgac attggtggtc ctgatcaaga atttggtgtg
3421 gacgttggcc ctgtttgctt tttatgtaca gactacaaag accatgacgg tgattataaa
3481 gatcatgaca tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg
3541 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
3601 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
3661 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
3721 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt
3781 gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt
3841 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
3901 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga
3961 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
4021 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
4081 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
4141 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
4201 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4261 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
4321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
4441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4501 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
4561 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
4621 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
4681 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
4741 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
4801 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
4861 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
4921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
4981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
5041 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
5101 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
5161 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
5221 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
5281 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
5341 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
5401 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
5461 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
5521 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
5581 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
5641 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
5701 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
5761 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
5821 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
5881 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
5941 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
6001 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
6061 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
6121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
6181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
6241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
6301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
6361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
6421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
6481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
6541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
6601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
6661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
6721 tgctcacatg t
//
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