| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-MYH9-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001585 |
| 产品用途: | 3' AAVLINK plasmid for MYH9 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MYH9 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-MYH9-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-MYH9-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-MYH9-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-MYH9-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-MYH9-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-MYH9-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-MYH9-FLAG 6658 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6658)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6658
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3363
/codon_start=1
/label=MYH9
/translation="VEDEKNSFREQLEEEEEAKHNLEKQIATLHAQVADMKKKMEDSVG
CLETAEEVKRKLQKDLEGLSQRHEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRQSAC
NLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLARALEEAMEQKAELE
RLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQQVEEMKTQLEELEDELQATEDAK
LRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDERKQRSMAVAARKK
LEMDLKDLEAHIDSANKNRDEAIKQLRKLQAQMKDCMRELDDTRASREEILAQAKENEK
KLKSMEAEMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIA
QLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELK
VKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQ
VDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADA
MNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE"
misc_feature 1519..1524
/label=SDS
misc_feature 1522..1527
/label=SDS
misc_feature 1525..1530
/label=SDS
misc_feature 1528..1533
/label=SDS
misc_feature 1633..1638
/label=SDS
misc_feature 2539..2544
/label=SDS
misc_feature 2686..2691
/label=SDS
misc_feature 2698..2703
/label=SDS
misc_feature 3121..3126
/label=SDS
misc_feature 3133..3138
/label=SDS
CDS 3366..3431
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3447..3878
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3591..3596
/label=SDS
repeat_region 3921..4061
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3921..4050
/label=AAV2 ITR
rep_origin 4136..4591
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4873..4977
/gene="bla"
/label=AmpR promoter
CDS 4978..5838
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6009..6597
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtggaggac gagaagaatt
1501 ccttccggga gcagctggag gaggaggagg aggccaagca caacctggag aagcagatcg
1561 ccaccctcca tgcccaggtg gccgacatga aaaagaagat ggaggacagt gtggggtgcc
1621 tggaaactgc tgaggaggtg aagaggaagc tccagaagga cctggagggc ctgagccagc
1681 ggcacgagga gaaggtggcc gcctacgaca agctggagaa gaccaagacg cggctgcagc
1741 aggagctgga cgacctgctg gtggacctgg accaccagcg ccagagcgcg tgcaacctgg
1801 agaagaagca gaagaagttt gaccagctcc tggcggagga gaagaccatc tctgccaagt
1861 atgcagagga gcgcgaccgg gctgaggcgg aggcccgaga gaaggagacc aaggctctgt
1921 cgctggcccg ggccctggag gaagccatgg agcagaaggc ggagctggag cggctcaaca
1981 agcagttccg cacggagatg gaggacctta tgagctccaa ggatgatgtg ggcaagagtg
2041 tccacgagct ggagaagtcc aagcgggccc tagagcagca ggtggaggag atgaagacgc
2101 agctggaaga gctggaggac gagctgcagg ccaccgaaga tgccaagctg cggttggagg
2161 tcaacctgca ggccatgaag gcccagttcg agcgggacct gcagggccgg gacgagcaga
2221 gcgaggagaa gaagaagcag ctggtcagac aggtgcggga gatggaggca gagctggagg
2281 acgagaggaa gcagcgctcg atggcagtgg ccgcccggaa gaagctggag atggacctga
2341 aggacctgga ggcgcacatc gactcggcca acaagaaccg ggacgaagcc atcaaacagc
2401 tgcggaagct gcaggcccag atgaaggact gcatgcgcga gctggatgac acccgcgcct
2461 ctcgtgagga gatcctggcc caggccaaag agaacgagaa gaagctgaag agcatggagg
2521 ccgagatgat ccagttgcag gaggaactgg cagccgcgga gcgtgccaag cgccaggccc
2581 agcaggagcg ggatgagctg gctgacgaga tcgccaacag cagcggcaaa ggagccctgg
2641 cgttagagga gaagcggcgt ctggaggccc gcatcgccca gctggaggag gagctggagg
2701 aggagcaggg caacacggag ctgatcaacg accggctgaa gaaggccaac ctgcagatcg
2761 accagatcaa caccgacctg aacctggagc gcagccacgc ccagaagaac gagaatgctc
2821 ggcagcagct ggaacgccag aacaaggagc ttaaggtcaa gctgcaggag atggagggca
2881 ctgtcaagtc caagtacaag gcctccatca ccgccctcga ggccaagatt gcacagctgg
2941 aggagcagct ggacaacgag accaaggagc gccaggcagc ctgcaaacag gtgcgtcgga
3001 ccgagaagaa gctgaaggat gtgctgctgc aggtggatga cgagcggagg aacgccgagc
3061 agtacaagga ccaggccgac aaggcatcta cccgcctgaa gcagctcaag cggcagctgg
3121 aggaggccga agaggaggcc cagcgggcca acgcctcccg ccggaaactg cagcgcgagc
3181 tggaggacgc cactgagacg gccgatgcca tgaaccgcga agtcagctcc ctaaagaaca
3241 agctcaggcg cggggacctg ccgtttgtcg tgccccgccg aatggcccgg aaaggcgccg
3301 gggatggctc cgacgaagag gtagatggca aagcggatgg ggctgaggcc aaacctgccg
3361 aaaaggacta caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg
3421 acgatgacaa gtaagaattc tgatgactcg agataatcaa cctctggatt acaaaatttg
3481 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
3541 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
3601 taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
3661 gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga
3721 tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt
3781 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
3841 tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca
3901 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
3961 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
4021 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
4081 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
4141 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
4201 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
4261 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
4321 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
4381 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4441 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
4501 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4561 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
4621 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
4681 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
4741 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
4801 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
4861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
4921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
4981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
5041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
5101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
5161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
5221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
5281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
5341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
5401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
5461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
5521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
5581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
5641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
5701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
5761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
5821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
5881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
5941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
6001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
6121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
6181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
6241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
6301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
6361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
6601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
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