| 质粒名称: | pMito-mfRFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000333 |
| 产品用途: | Expresses Mito-mfRFP in mammalian cells culture |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | N1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | COX8A, mfRFP |
| 物种来源: | Human, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A Set of Novel Far-Red Fluorescent Proteins for Temporal Domain Multiplexing and Super-Resolution Imaging
Olumayowa F., Rong Z., Wang R., Papadaki S., Wang X., Cao J., ubach F.V., Koster R.W., Namikawa K., Piatkevich K.D.
bioRxiv 2025.06.01.657196 (2025)
|
pMito-mfRFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pMito-mfRFP载体质粒基本信息
pMito-mfRFP质粒图谱载体图谱和pMito-mfRFP载体序列质粒序列多克隆位点信息
pMito-mfRFP质粒载体简介
pMito-mfRFP质粒序列载体序列
LOCUS pMito-mfRFP 4773 bp DNA circular SYN 26-DEC-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4773)
AUTHORS Shcherbakova DM, Verkhusha VV
TITLE Near-infrared fluorescent proteins for multicolor in vivo imaging.
JOURNAL Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.
PUBMED 23770755
REFERENCE 2 (bases 1 to 4773)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521."
FEATURES Location/Qualifiers
source 1..4773
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 597..683
/codon_start=1
/label=COX8A
/translation="MSVLTPLLLRGLTGSARRLPVPRAKIHSL"
CDS 705..1436
/codon_start=1
/label=mfRFP
/translation="MVSKGEELIKENMPLKLYMEGTVNNHHFKCTAEGEGKPYEGTQTQ
RIKVVEGGPLPFAFDILATSFMYGSHTFIDHTPGIPDFWKQSFPEGFTWERVTTYEDGG
VLIATQDTSLHDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEAHTELLYPTDGGLEGRTA
LALKLVGGGHLLCNFKTIYRSKKPAKNLKMPGVYYVDYRLERIEEADNETYVEQHEVAV
ARYCDLPTKLGHKLKGMDELYK"
primer_bind complement(1438..1466)
/label=2022.10.12 pMito-E2Crimson R
polyA_signal 1561..1682
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1598..1617)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1652..1671
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1689..2144)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1826..1847)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2038..2057
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2171..2275
/gene="bla"
/label=AmpR promoter
promoter 2277..2634
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2300..2320)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2485..2620
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2547..2566
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2570..2581)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2669..3463
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2723..2742)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3333..3352
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3651..3670)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3695..3742
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4071..4659
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4560..4579
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt
601 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc
661 cgcgcgccaa gatccattcg ttgggggatc caccggtacc ggtgatggtg agcaagggcg
721 aggagctgat taaggagaac atgccactga agctgtacat ggagggcacc gttaacaatc
781 accacttcaa gtgcacagct gagggcgaag gcaagcccta cgagggcacc cagacccaga
841 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagtt
901 tcatgtacgg cagtcacacc ttcatcgacc acaccccggg catccccgac ttctggaagc
961 agtccttccc cgagggcttc acgtgggaga gagtcaccac atacgaagac gggggcgtcc
1021 tgatcgcgac ccaggacacc agcctccatg acggctgcct catctacaac gtcaagttga
1081 gaggggtgaa cttcccatcc aacggccctg tgatgcaaaa gaaaacactc ggctgggagg
1141 cacacaccga gcttttgtac cccactgacg gcggtctgga aggcagaacc gcgctggccc
1201 tgaagcttgt gggcgggggc cacctattgt gtaacttcaa gactatatac agatccaaga
1261 aacccgctaa gaacctcaag atgcccggcg tctactatgt ggactacaga ctggaaagaa
1321 tcgaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg gccagatact
1381 gcgacctccc taccaaactg gggcacaagc ttaagggcat ggacgagctg tacaagtagg
1441 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
1501 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
1561 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
1621 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
1681 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
1741 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
1801 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
1861 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
1921 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
1981 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
2041 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
2101 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
2161 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
2221 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
2281 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
2341 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
2401 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
2461 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
2521 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
2581 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
2641 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
2701 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
2761 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
2821 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
2881 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
2941 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
3001 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
3061 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
3121 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
3181 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
3241 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
3301 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
3361 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
3421 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
3481 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
3541 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
3601 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
3661 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
3721 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
3781 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
3841 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
3901 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
3961 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
4021 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
4081 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
4141 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
4201 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
4261 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
4321 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
4381 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
4441 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
4501 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
4561 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
4621 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
4681 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
4741 gattctgtgg ataaccgtat taccgccatg cat
//
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