pSL1189

LOCUS      pSL1189            6000 bp DNA     circular SYN 11-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6000
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..938
                     /label=oSCL2177
     primer_bind     900..957
                     /label=oQX1609_KRAS-G12R-recF2
     primer_bind     900..957
                     /label=oQX1614_KRAS-G12A-recF2
     primer_bind     900..926
                     /label=oQX1608_BamHI-KRAS-recF
     primer_bind     901..959
                     /label=oQX1616_KRAS-G13D-recF2
     primer_bind     901..958
                     /label=oQX1618_KRAS-G13C-recF2
     gene            907..1500
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /label=KRAS
                     /note="KRAS proto-oncogene, GTPase"
                     /db_xref="GeneID:3845"
                     /db_xref="HGNC:HGNC:6407"
                     /db_xref="MIM:190070"
     CDS             907..1476
                     /codon_start=1
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /product="GTPase KRas isoform a"
                     /EC_number="3.6.5.2"
                     /label=KRAS
                     /note="isoform a is encoded by transcript variant a;
                     v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; 
                     transforming protein p21; c-Kirsten-ras protein; K-ras p21 
                     protein; oncogene KRAS2; PR310 c-K-ras oncogene; cellular 
                     c-Ki-ras2 proto-oncogene; Kirsten rat sarcoma viral 
                     proto-oncogene; GTPase KRas; Kirsten rat sarcoma viral 
                     oncogene homolog; cellular transforming proto-oncogene; 
                     proto-oncogene GTPase"
                     /db_xref="CCDS:CCDS8703.1"
                     /db_xref="GeneID:3845"
                     /db_xref="HGNC:HGNC:6407"
                     /db_xref="MIM:190070"
                     /protein_id="NP_203524.1"
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQRVEDAFYTLVREI
                     RQYRLKKISKEEKTPGCVKIKKCIIM"
     mat_peptide     907..1464
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /product="GTPase KRas. /id=PRO_0000082641"
                     /note="propagated from UniProtKB/Swiss-Prot (P01116.1)"
     exon            907..1017
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /inference="alignment:same species:2.1.0"
     misc_feature    907..909
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /note="N-acetylmethionine, in GTPase KRas, alternate. 
                     /evidence=ECO:0000269|Ref.17; propagated from 
                     UniProtKB/Swiss-Prot (P01116.1); acetylation site"
     mat_peptide     910..1464
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /product="GTPase KRas, N-terminally processed.
                     /id=PRO_0000326480"
                     /note="propagated from UniProtKB/Swiss-Prot (P01116.1)"
     misc_feature    910..912
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /note="N-acetylthreonine, in GTPase KRas, N-terminally 
                     processed. /evidence=ECO:0000269|Ref.17; propagated from 
                     UniProtKB/Swiss-Prot (P01116.1); acetylation site"
     primer_bind     940..963
                     /label=oQX1617_KRAS-G13D-recF1
     primer_bind     940..961
                     /label=oQX1619_KRAS-G13C-recF1
     primer_bind     943..960
                     /label=oQX1610_KRAS-G12R-recF1
     primer_bind     943..960
                     /label=oQX1615_KRAS-G12A-recF1
     misc_feature    1000..1026
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /note="propagated from UniProtKB/Swiss-Prot (P01116.1);
                     Region: Effector region"
     misc_feature    1009..1011
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /note="(Microbial infection) O-linked (Glc) threonine, by 
                     P.sordellii toxin TcsL. 
                     /evidence=ECO:0000269|PubMed:19744486; propagated from 
                     UniProtKB/Swiss-Prot (P01116.1); glycosylation site"
     exon            1018..1196
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(1066..1100)
                     /label=oQX1626_KRAS-Q61R-recR
     primer_bind     complement(1069..1096)
                     /label=oQX1623_KRAS-Q61H-recR
     primer_bind     1077..1111
                     /label=oQX1622_KRAS-Q61H-recF
     primer_bind     1077..1110
                     /label=oQX1625_KRAS-Q61R-recF
     primer_bind     1090..1111
                     /label=oQX1624_KRAS-F
     exon            1197..1500
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1216..1218
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000269|PubMed:22711838; propagated from 
                     UniProtKB/Swiss-Prot (P01116.1); acetylation site"
     primer_bind     1320..1365
                     /label=oQX1620_KRAS-A146T-recF
     primer_bind     complement(1320..1361)
                     /label=oQX1621_KRAS-A146T-recR
     primer_bind     complement(1337..1376)
                     /label=oSCL2201
     primer_bind     1357..1434
                     /label=oSCL2202
     primer_bind     complement(1402..1480)
                     /label=oSCL2203
     misc_feature    1402..1461
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /note="propagated from UniProtKB/Swiss-Prot (P01116.1);
                     Region: Hypervariable region"
     primer_bind     complement(1441..1547)
                     /label=oQX1612_EcoRI-KRAS-recR
     primer_bind     complement(1441..1476)
                     /label=oQX1611_KRAS-R
     primer_bind     1461..1502
                     /label=oSCL2200
     misc_feature    1462..1464
                     /gene="KRAS"
                     /gene_synonym="'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;
                     CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; 
                     KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2"
                     /note="Cysteine methyl ester. 
                     /evidence=ECO:0000269|PubMed:27791178, 
                     ECO:0007744|PDB:5TAR, ECO:0007744|PDB:5TB5; propagated from
                     UniProtKB/Swiss-Prot (P01116.1); methylation site"
     primer_bind     complement(1478..1520)
                     /label=oSCL2178
     primer_bind     complement(1525..1548)
                     /label=oQX1613_3.1-EcoRI-recR
     terminator      1597..1824
                     /label=bGH PA
     misc_feature    2492..2569
                     /label=SV40 ORI
     CDS             2711..3502
                     /label=Kan/neoR
     primer_bind     complement(3842..3862)
                     /label=M13-rev
     misc_binding    complement(3868..3890)
                     /label=LacO
     promoter        complement(3895..3924)
                     /label=lac
     CDS             complement(5007..5666)
                     /label=AmpR
     promoter        complement(5906..5934)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatga ctgaatataa acttgtggta gttggagctg gtggcgtagg caagagtgcc
      961 ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat
     1021 tcctacagga agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca
     1081 gcaggtcaag aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt
     1141 ctttgtgtat ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa
     1201 caaattaaaa gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt
     1261 gatttgcctt ctagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga
     1321 attcctttta ttgaaacatc agcaaagaca agacagagag tggaggatgc tttttataca
     1381 ttggtgagag agatccgaca atacagattg aaaaaaatca gcaaagaaga aaagactcct
     1441 ggctgtgtga aaattaaaaa atgcattata atgtaatctg ggtgttgatg atgccttcta
     1501 ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg
     1561 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     1621 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1681 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1741 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     1801 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     1861 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1921 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1981 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2041 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2101 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2161 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2221 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2281 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     2341 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     2401 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     2461 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     2521 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2581 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     2641 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     2701 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     2761 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     2821 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     2881 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     2941 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     3001 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     3061 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     3121 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     3181 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     3241 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     3301 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     3361 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     3421 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     3481 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     3541 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     3601 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     3661 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3721 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3781 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     3841 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3901 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3961 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     4021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag
     4801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4981 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5041 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5101 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5161 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5221 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5281 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5341 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5401 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5461 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5521 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5581 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5641 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5701 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5761 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5821 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5881 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5941 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//