pAAVLINK-EF1a-HA-5'-CDK5RAP2

LOCUS      pAAVLINK-EF1a-HA-5'-CDK5RAP2              7360 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4500
                     /codon_start=1
                     /label=CDK5RAP2
                     /translation="MMDLVLEEDVTVPGTLSGCSGLVPSVPDDLDGINPNAGLGNGLLP
                     NVSEETVSPTRARNMKDFENQITELKKENFNLKLRIYFLEERMQQEFHGPTEHIYKTNI
                     ELKVEVESLKRELQEREQLLIKASKAVESLAEAGGSEIQRVKEDARKKVQQVEDLLTKR
                     ILLLEKDVTAAQAELEKAFAGTETEKALRLRLESKLSEMKKMHEGDLAMALVLDEKDRL
                     IEELKLSLKSKEALIQCLKEEKSQMACPDENVSSGELRGLCAAPREEKERETEAAQMEH
                     QKERNSFQERIQALEEDLREKEREIATEKKNSLKRDKAIQGLTMALKSKEKKVEELNSE
                     IEKLSAAFAKAREALQKAQTQEFQGSEDYETALSGKEALSAALRSQNLTKSTENHRLRR
                     SIKKITQELSDLQQERERLEKDLEEAHREKSKGDCTIRDLRNEVEKLRNEVNEREKAME
                     NRYKSLLSESNKKLHNQEQVIKHLTESTNQKDVLLQKFNEKDLEVIQQNCYLMAAEDLE
                     LRSEGLITEKCSSQQPPGSKTIFSKEKKQSSDYEELIQVLKKEQDIYTHLVKSLQESDS
                     INNLQAELNKIFALRKQLEQDVLSYQNLRKTLEEQISEIRRREEESFSLYSDQTSYLSI
                     CLEENNRFQVEHFSQEELKKKVSDLIQLVKELYTDNQHLKKTIFDLSCMGFQGNGFPDR
                     LASTEQTELLASKEDEDTIKIGEDDEINFLSDQHLQQSNEIMKDLSKGGCKNGYLRHTE
                     SKISDCDGAHAPGCLEEGAFINLLAPLFNEKATLLLESRPDLLKVVRELLLGQLFLTEQ
                     EVSGEHLDGKTEKTPKQKGELVHFVQTNSFSKPHDELKLSCEAQLVKAGEVPKVGLKDA
                     SVQTVATEGDLLRFKHEATREAWEEKPINTALSAEHRPENLHGVPGWQAALLSLPGITN
                     REAKKSRLPILIKPSRSLGNMYRLPATQEVVTQLQSQILELQGELKEFKTCNKQLHQKL
                     ILAEAVMEGRPTPDKTLLNAQPPVGAAYQDSPGEQKGIKTTSSVWRDKEMDSDQQRSYE
                     IDSEICPPDDLASLPSCKENPEDVLSPTSVATYLSSKSQPSAKVSVMGTDQSESINTSN
                     ETEYLKQKIHDLETELEGYQNFIFQLQKHSQCSEAIITVLCGTEGAQDGLSKPKNGSDG
                     EEMTFSSLHQVRYVKHVKILGPLAPEMIDSRVLENLKQQLEEQEYKLQKEQNLNMQLFS
                     EIHNLQNKFRDLSPPRYDSLVQSQA"
     RBS             1469..1480
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    2868..2873
                     /label=SDS
     misc_feature    2971..2976
                     /label=SDS
     misc_feature    3511..3516
                     /label=SDS
     misc_feature    3621..3626
                     /label=SDS
     intron          4501..4541
                     /label=Splicing Donor
     misc_feature    4545..4578
                     /label=lox2272-JT1571
     repeat_region   4623..4763
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4623..4752
                     /label=AAV2 ITR
     rep_origin      4838..5293
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5575..5679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5680..6540
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6711..7299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat gatggacttg gtgttggaag aggacgtcac
      781 cgtccctggg acgctcagcg gctgcagtgg ccttgttccc agtgtaccag atgacctgga
      841 tggcatcaac cccaatgctg ggttgggaaa tggtctgctc ccaaatgtgt cagaagaaac
      901 agtgtctccc accagagcac ggaacatgaa ggactttgaa aatcaaatca ctgaattgaa
      961 gaaagaaaac tttaacctaa agctccgcat ctatttcctt gaggaaagaa tgcaacagga
     1021 atttcatggc cccactgaac atatctacaa aactaacatt gagctcaagg tggaagtaga
     1081 aagtctgaag cgggaactcc aggagagaga gcagctgctc atcaaagcct ccaaagcagt
     1141 tgagagctta gctgaagcag gtggctctga aatccagcgg gtgaaagaag atgctcgaaa
     1201 gaaggtgcag caggtggaag atctcctaac taaaagaata ctccttttgg aaaaggatgt
     1261 gacagccgcc caggcagaac tggaaaaggc ctttgcaggg acagagacgg agaaggctct
     1321 tcggttgcgt ttggaaagca agctttcaga gatgaagaag atgcacgagg gggacttggc
     1381 gatggctctg gtcctggatg agaaagacag actgattgag gagttgaagc tgtctttgaa
     1441 gagcaaagaa gctttaattc agtgccttaa agaggagaaa tctcagatgg catgtcctga
     1501 tgagaatgtg tcatctggag agctccgagg actttgtgct gctccaaggg aagaaaagga
     1561 gagagaaact gaggctgcac aaatggagca tcagaaggag agaaacagct ttcaagagag
     1621 gatccaggca cttgaagagg acctgagaga gaaggaaaga gaaattgcta cagagaagaa
     1681 aaatagtcta aagagggata aagccattca gggtttaacc atggcattaa aatcaaagga
     1741 aaaaaaggtt gaagaactta actctgaaat tgaaaagctc agtgctgcct ttgctaaagc
     1801 cagagaggcc ctacagaaag cacagaccca ggaatttcag gggtctgaag actatgagac
     1861 tgctctatca ggaaaggaag ccctttcggc tgcgctgcgc tcacaaaacc tcaccaagag
     1921 tacagagaac cacagactgc gtagaagcat taagaagatc acccaggagc tgagtgactt
     1981 gcagcaggag agggagagac tggagaagga cctggaggaa gcccatcgag agaagagcaa
     2041 aggagactgc accatccgtg atcttagaaa tgaagttgaa aaattacgca atgaagtgaa
     2101 tgaaagagag aaagcaatgg aaaatcgtta caagagtctt ctgagtgaaa gcaataaaaa
     2161 attgcacaat caagagcaag tgatcaaaca tctaacagaa agtaccaatc agaaggacgt
     2221 gttgcttcag aaattcaatg aaaaagattt ggaagtaata cagcagaact gctatttaat
     2281 ggctgcagag gatcttgagc tcaggagtga aggcttaata acagaaaagt gctcttctca
     2341 acagccacca ggcagcaaaa ccatcttctc taaggaaaag aaacaatcat cagactatga
     2401 agagctgatt caggtcttaa agaaagagca ggacatctat acccatctgg tcaaatctct
     2461 gcaggaatca gacagtatca acaacctgca ggctgagtta aacaagattt ttgccctgcg
     2521 gaagcaactg gagcaggatg tgctttcata tcagaatttg cggaagacct tggaggagca
     2581 gatcagcgaa attcggaggc gggaagaaga atcattttca ctttatagtg atcaaacatc
     2641 ttatctaagt atttgccttg aagaaaacaa tcggtttcaa gtggaacatt tttctcaaga
     2701 agaacttaag aaaaaggtca gtgaccttat acagctagtg aaggagctgt atacagacaa
     2761 ccagcacctg aagaaaacca tttttgatct ctcctgcatg ggtttccagg gaaatgggtt
     2821 tccagataga cttgcgtcta cagaacaaac agagcttctg gctagcaagg aggacgagga
     2881 cacgatcaaa attggggagg atgacgagat taatttcctg agtgaccagc atttgcagca
     2941 gagtaatgag attatgaaag acctttccaa aggaggctgc aaaaatggat acttaaggca
     3001 cacggagtct aagatttcag attgtgatgg ggcccacgca cctggctgcc tagaagaagg
     3061 tgcattcata aacctgcttg cccctttgtt caatgagaag gccacattat tactggaatc
     3121 caggccagac cttctgaaag tggtacggga actgcttctg ggacaactat tcttgacaga
     3181 gcaggaagtt tctggagaac accttgatgg taaaactgag aagacaccta agcaaaaagg
     3241 tgaacttgta cattttgtcc aaaccaactc attttccaag ccacatgatg aactgaagtt
     3301 gtcttgtgag gcccagctag taaaggcagg cgaagtgccc aaggtaggac tgaaagatgc
     3361 ctcagtgcag actgtggcca cggagggcga cctgctgaga ttcaagcatg aagcaacaag
     3421 agaggcttgg gaagagaaac cgatcaacac tgcactcagc gcagagcatc ggccagagaa
     3481 cctgcacggg gtgcctgggt ggcaggctgc cctcctttcc ctccctggta ttaccaacag
     3541 agaggctaag aagtcccgct tgccaatcct aataaaacca tcccggtcat taggaaatat
     3601 gtatcgtctc cctgccaccc aggaggtggt gacgcagctg cagagccaga tcttggagct
     3661 gcagggggag ctgaaggagt ttaaaacttg taataagcaa cttcaccaaa agttaattct
     3721 ggctgaagca gtgatggagg ggaggccaac gcccgacaaa acgttgctga atgctcagcc
     3781 ccctgtggga gcagcctacc aggacagccc aggagagcag aaaggaatta aaaccacatc
     3841 ttctgtctgg agagacaagg aaatggacag tgatcagcaa agaagctacg agattgactc
     3901 tgagatttgc ccacctgatg accttgccag cttgccatca tgcaaagaaa atcctgaaga
     3961 tgttctgagc ccaacttcag tagctactta cctgagttcc aagagtcagc cttctgctaa
     4021 agtcagtgtg atggggactg atcagtcaga gagcattaat acctcaaatg agacagaata
     4081 cttaaaacag aaaatccatg acttggaaac tgagctggaa ggctaccaga atttcatatt
     4141 tcagcttcaa aagcactccc agtgcagtga ggccataatt acagttttgt gtgggacaga
     4201 aggggcccag gatggcttga gcaagcccaa gaatggttct gatggggaag aaatgacctt
     4261 ttcaagtttg caccaagtgc gatacgtgaa acacgtgaaa atcctcggtc cgctggcccc
     4321 agagatgatt gacagcaggg tgctggagaa cctcaaacag cagctggagg aacaggaata
     4381 caagctgcag aaggagcaga atttgaacat gcaacttttc agtgagatcc ataatctgca
     4441 gaataagttc agagatctct cacctcccag atacgattca ttagttcagt cccaagccag
     4501 gtaagtatca aggttacaag acaggtttaa ggagaccaat aagctattat tcgtatagga
     4561 tactttatac gaagttatta agttatggag accggtgtgg cacgtgcgga ccgagcggcc
     4621 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4681 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4741 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     4801 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     4861 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     4921 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4981 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5041 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5101 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5161 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     5221 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5281 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5341 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5401 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5461 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5521 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5581 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5641 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5701 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5761 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5821 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5881 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5941 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6001 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6061 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6121 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6181 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6241 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6301 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6361 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6421 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6481 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6541 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6601 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6721 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6781 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6841 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6901 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6961 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7021 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7081 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7141 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7201 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7261 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7321 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//