psiCHECK-2-h1-cluster01

LOCUS       psiCHECK-2-h1-cluster01            6611 bp DNA     circular SYN 16-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2010
                     /label=h1-cluster01
     polyA_signal    2026..2074
                     /note="synthetic polyadenylation signal"
     promoter        2083..2834
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2864..2873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2870..4522
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3829..3837
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4566..4687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4820..4924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4925..5785
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5956..6544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 ccgccctcca ggtaaggaag cgggactggc ggcgcggcca cctcggcggt gcctatctgc
     1741 tcgaatccat cccaaagatt ttcttctcga tacacttggg ttttagtggt gggagtctga
     1801 gacctaggga gggatccccg ggtggcctat tgtgagaaat atgagactac atgtgcatct
     1861 cctggaaaag cactttgcac agcgcccgtg atttagagcc tggagttgat gttgggcatc
     1921 ccccatcttc ttgcttcctc tttccttctc ttgttctctc tcctccgtcc cccaccccca
     1981 atctcaatct tttctctctt tctctcccac gcggccgctg gccgcaataa aatatcttta
     2041 ttttcattac atctgtgtgt tggttttttg tgtgaggatc taaatgagtc ttcggacctc
     2101 gcgggggccg cttaagcggt ggttagggtt tgtctgacgc ggggggaggg ggaaggaacg
     2161 aaacactctc attcggaggc ggctcggggt ttggtcttgg tggccacggg cacgcagaag
     2221 agcgccgcga tcctcttaag cacccccccg ccctccgtgg aggcgggggt ttggtcggcg
     2281 ggtggtaact ggcgggccgc tgactcgggc gggtcgcgcg ccccagagtg tgaccttttc
     2341 ggtctgctcg cagacccccg ggcggcgccg ccgcggcggc gacgggctcg ctgggtccta
     2401 ggctccatgg ggaccgtata cgtggacagg ctctggagca tccgcacgac tgcggtgata
     2461 ttaccggaga ccttctgcgg gacgagccgg gtcacgcggc tgacgcggag cgtccgttgg
     2521 gcgacaaaca ccaggacggg gcacaggtac actatcttgt cacccggagg cgcgagggac
     2581 tgcaggagct tcagggagtg gcgcagctgc ttcatccccg tggcccgttg ctcgcgtttg
     2641 ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc
     2701 ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt
     2761 cccaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc
     2821 agcgacccgc ttaaaagctt ggcattccgg tactgttggt aaagccacca tggccgatgc
     2881 taagaacatt aagaagggcc ctgctccctt ctaccctctg gaggatggca ccgctggcga
     2941 gcagctgcac aaggccatga agaggtatgc cctggtgcct ggcaccattg ccttcaccga
     3001 tgcccacatt gaggtggaca tcacctatgc cgagtacttc gagatgtctg tgcgcctggc
     3061 cgaggccatg aagaggtacg gcctgaacac caaccaccgc atcgtggtgt gctctgagaa
     3121 ctctctgcag ttcttcatgc cagtgctggg cgccctgttc atcggagtgg ccgtggcccc
     3181 tgctaacgac atttacaacg agcgcgagct gctgaacagc atgggcattt ctcagcctac
     3241 cgtggtgttc gtgtctaaga agggcctgca gaagatcctg aacgtgcaga agaagctgcc
     3301 tatcatccag aagatcatca tcatggactc taagaccgac taccagggct tccagagcat
     3361 gtacacattc gtgacatctc atctgcctcc tggcttcaac gagtacgact tcgtgccaga
     3421 gtctttcgac agggacaaaa ccattgccct gatcatgaac agctctgggt ctaccggcct
     3481 gcctaagggc gtggccctgc ctcatcgcac cgcctgtgtg cgcttctctc acgcccgcga
     3541 ccctattttc ggcaaccaga tcatccccga caccgctatt ctgagcgtgg tgccattcca
     3601 ccacggcttc ggcatgttca ccaccctggg ctacctgatt tgcggctttc gggtggtgct
     3661 gatgtaccgc ttcgaggagg agctgttcct gcgcagcctg caagactaca aaattcagtc
     3721 tgccctgctg gtgccaaccc tgttcagctt cttcgctaag agcaccctga tcgacaagta
     3781 cgacctgtct aacctgcacg agattgcctc tggcggcgcc ccactgtcta aggaggtggg
     3841 cgaagccgtg gccaagcgct ttcatctgcc aggcatccgc cagggctacg gcctgaccga
     3901 gacaaccagc gccattctga ttaccccaga gggcgacgac aagcctggcg ccgtgggcaa
     3961 ggtggtgcca ttcttcgagg ccaaggtggt ggacctggac accggcaaga ccctgggagt
     4021 gaaccagcgc ggcgagctgt gtgtgcgcgg ccctatgatt atgtccggct acgtgaataa
     4081 ccctgaggcc acaaacgccc tgatcgacaa ggacggctgg ctgcactctg gcgacattgc
     4141 ctactgggac gaggacgagc acttcttcat cgtggaccgc ctgaagtctc tgatcaagta
     4201 caagggctac caggtggccc cagccgagct ggagtctatc ctgctgcagc accctaacat
     4261 tttcgacgcc ggagtggccg gcctgcccga cgacgatgcc ggcgagctgc ctgccgccgt
     4321 cgtcgtgctg gaacacggca agaccatgac cgagaaggag atcgtggact atgtggccag
     4381 ccaggtgaca accgccaaga agctgcgcgg cggagtggtg ttcgtggacg aggtgcccaa
     4441 gggcctgacc ggcaagctgg acgcccgcaa gatccgcgag atcctgatca aggctaagaa
     4501 aggcggcaag atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga
     4561 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4621 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4681 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     4741 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccaggtgg
     4801 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4861 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4921 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4981 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5041 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5101 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5161 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5221 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5281 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5341 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5401 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5461 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5521 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5581 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5641 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5701 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5761 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5821 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5881 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5941 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6001 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6061 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6121 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6181 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6241 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6301 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6361 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6421 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6481 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6541 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6601 catggctcga c
//