pAAVLINK-EF1alpha-HA-NIPBL-A

LOCUS       pAAVLINK-EF1alpha-HA-NIPBL-A            5789 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..2929
                     /codon_start=1
                     /gene="NIPBL"
                     /gene_synonym="CDLS; CDLS1; IDN3; IDN3-B; Scc2"
                     /product="nipped-B-like protein isoform A"
                     /label=NIPBL
                     /note="isoform A is encoded by transcript variant A;sister 
                     chromatid cohesion 2 homolog; nipped-B-like protein; SCC2 
                     homolog; delangin; Nipped-B homolog"
                     /db_xref="GI:47578105"
                     /db_xref="CCDS:CCDS3920.1"
                     /db_xref="GeneID:25836"
                     /db_xref="HGNC:HGNC:28862"
                     /db_xref="MIM:608667"
                     /protein_id="NP_597677.2"
                     /translation="NGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARI
                     AEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPN
                     VFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGN
                     RFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKV
                     SGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPA
                     GSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEK
                     AAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQT
                     SKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQI
                     SQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGAT
                     GGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAP
                     VSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSS
                     ENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSET
                     TKSRPETPKQKGESRPETPKQKSDGHPETPKQK"
     misc_feature    808..813
                     /label=SDS
     misc_feature    2280..2285
                     /label=SDS
     misc_feature    2299..2304
                     /label=SDS
     intron          2930..2970
                     /label=Splicing Donor
     misc_feature    2974..3007
                     /label=loxP-JT1571
     repeat_region   3052..3192
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3052..3181
                     /label=AAV2 ITR
     repeat_region   3053..3193
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3267..3722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4004..4108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4109..4969
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5140..5728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctaa tggggatatg ccccatgtcc ccattactac
      781 tcttgcgggg attgctagtc tcacagacct cctgaaccag ctgcctcttc catctccttt
      841 acctgctaca actacaaaga gccttctctt taatgcacga atagcagaag aggtgaactg
      901 ccttttggct tgtagggatg acaatttggt ttcacagctt gtccatagcc tcaaccaggt
      961 atcaacagat cacatagagt tgaaagataa ccttggcagt gatgacccag aaggtgacat
     1021 accagtcttg ttgcaggccg tcctggcaag gagtcctaat gttttcaggg agaaaagcat
     1081 gcagaacaga tatgtacaaa gtggaatgat gatgtctcag tataaacttt ctcagaattc
     1141 catgcacagt agtcctgcat cttccaatta tcaacaaacc actatctcac atagcccctc
     1201 cagccggttt gtgccaccac agacaagctc tgggaacaga tttatgccac agcaaaatag
     1261 cccagtgcct agtccatacg ccccacaaag ccctgcagga tacatgccat attcccatcc
     1321 ttcaagttac acaacacatc cacagatgca acaagcatcg gtatcaagtc ccattgttgc
     1381 aggtggtttg agaaacatac atgataataa agtttctggt ccgttgtctg gcaattcagc
     1441 taatcatcat gctgataatc ctagacatgg ttcaagtgag gactacctac acatggtgca
     1501 caggctaagt agtgacgatg gagattcttc aacaatgagg aatgctgcat cttttccctt
     1561 gagatctcca cagccagtat gctcccctgc tggaagtgaa ggaactccta aaggctcaag
     1621 accaccttta atcctacaat ctcagtctct accttgttca tcacctcgag atgttccacc
     1681 agatatcttg ctagattctc cagaaagaaa acaaaagaag cagaagaaaa tgaaattagg
     1741 caaggatgaa aaagagcaga gtgagaaagc ggcaatgtat gatataatta gttctccatc
     1801 caaggactct actaaactta cattaagact ttctcgtgta aggtcttcag acatggacca
     1861 gcaagaggat atgatttctg gtgtggaaaa tagcaatgtt tcagaaaatg atattccttt
     1921 taatgtgcag tacccaggac agacttcaaa aacacccatt actccacaag atataaaccg
     1981 cccactaaat gctgctcaat gtttgtcgca gcaagaacaa acagcattcc ttccagcaaa
     2041 tcaagtgcct gttttacaac agaacacttc agttgctgca aaacaacccc agacttctgt
     2101 ggtacagaat caacaacaga tatcacaaca gggacctata tatgatgaag tggaattgga
     2161 tgcattggct gaaattgagc gaatagagag agaatcagct attgaaaggg agcgcttctc
     2221 aaaagaagtt caagataaag ataagccttt gaaaaaaaga aaacaagatt cttacccaca
     2281 ggaggctggg ggtgctacag gaggtaatag accagcttct caggagacgg gttctacggg
     2341 aaatgggtca aggccagcat taatggttag cattgatctt catcaggcag gaagagtgga
     2401 ctctcaggct tctataactc aggattcaga ctccataaaa aagcctgaag aaatcaaaca
     2461 atgtaatgat gcacctgttt ctgttcttca ggaagatatt gttggaagtc ttaaatctac
     2521 accagaaaac catcctgaga cacctaaaaa aaagtctgat cctgagcttt caaagagtga
     2581 aatgaaacaa agtgaaagta gattagcaga atctaaacca aatgaaaacc gattggtgga
     2641 gacaaaatca agtgaaaata agttagaaac taaagttgag acccaaacag aagaacttaa
     2701 acagaatgag agcagaacaa ctgaatgcaa acaaaacgag agcaccatag ttgagcctaa
     2761 acaaaatgaa aatagactgt ctgacacaaa accaaatgac aacaaacaaa ataatggcag
     2821 atcagaaaca acaaaatcaa ggcctgaaac cccaaagcaa aagggtgaaa gccggcctga
     2881 gactccaaaa caaaagagtg atgggcatcc tgaaacccca aaacagaagg taagtatcaa
     2941 ggttacaaga caggtttaag gagaccaata agctattatt cgtatagcat acattatacg
     3001 aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
     3061 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     3121 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     3181 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3241 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     3301 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     3361 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3421 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3481 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3541 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     3601 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     3661 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     3721 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3781 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3841 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3901 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3961 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4021 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4081 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4141 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4201 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4261 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4321 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4381 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4441 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4501 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4561 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4621 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4681 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4741 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4801 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4861 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4921 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4981 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5041 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5761 ggccttttgc tggccttttg ctcacatgt
//