| 质粒名称: | PCDH-CMV-mCherry-TRIM25 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001256 |
| 产品用途: | Express proteins in cells |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH-CMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TRIM25 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Coiled-coil-domain-mediated TRIM E3 protein condensation modulates enzymatic activity and functional specificity
Qi Chen, Peiguo Yang
Cell Reports, 115766, June 24, 2025
|
PCDH-CMV-mCherry-TRIM25
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
PCDH-CMV-mCherry-TRIM25载体质粒基本信息
PCDH-CMV-mCherry-TRIM25质粒图谱载体图谱和PCDH-CMV-mCherry-TRIM25载体序列质粒序列多克隆位点信息
PCDH-CMV-mCherry-TRIM25质粒载体简介
PCDH-CMV-mCherry-TRIM25质粒序列载体序列
LOCUS PCDH-CMV-mCherry-TRIM25 6616 bp DNA circular SYN 29-JUL-2025
DEFINITION Vector for fusing mCherry to the N-terminus of a partner protein.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6616)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Aug 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6616
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1320
/codon_start=1
/product="monomeric derivative of DsRed fluorescent
protein"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind 1329..1348
/label=F
primer_bind 1340..1369
/label=TRIM25-F
misc_feature 1350
/label=added
misc_feature 1351..3243
/label=TRIM25
primer_bind complement(3224..3243)
/label=TRIM25-R
primer_bind complement(3244..3263)
/label=R
misc_feature 3289..3299
/label=stop codons
/note="stop codons in all three reading frames"
polyA_signal 3404..3525
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3532..3987)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4014..4118
/gene="bla"
/label=AmpR promoter
promoter 4120..4477
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4328..4463
/label=SV40 ori
/note="SV40 origin of replication"
CDS 4512..5306
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 5538..5585
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 5914..6502
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
1321 tccggactca gatctcgagc tcaagcttca atggcagagc tgtgccccct ggccgaggag
1381 ctgtcgtgct ccatctgcct ggagcccttc aaggagccgg tcaccactcc gtgcggccac
1441 aacttctgcg ggtcgtgcct gaatgagacg tgggcagtcc agggctcgcc atacctgtgc
1501 ccgcagtgcc gcgccgtcta ccaggcgcga ccgcagctgc acaagaacac ggtgctgtgc
1561 aacgtggtgg agcagttcct gcaggccgac ctggcccggg agccacccgc cgacgtctgg
1621 acgccgcccg cccgcgcctc tgcacccagc ccgaatgccc aggtggcctg cgaccactgc
1681 ctgaaggagg ccgccgtgaa gacgtgcttg gtgtgcatgg cctccttctg tcaggagcac
1741 ctgcagccgc acttcgacag ccccgccttc caggaccacc cgctgcagcc gcccgttcgc
1801 gacctgttgc gccgcaaatg ttcccagcac aatcggctgc gggaattttt ctgccccgag
1861 cacagcgagt gcatctgcca catctgcctg gtggagcata agacctgctc tcccgcgtcc
1921 ctgagccagg ccagcgccga cctggaggcc accctgaggc acaaactaac tgtcatgtac
1981 agtcagatca acggggcgtc gagagcactg gatgatgtga gaaacaggca gcaggatgtg
2041 cggatgactg caaacagaaa ggtggagcag ctacaacaag aatacacgga aatgaaggct
2101 ctcttggacg cctcagagac cacctcgaca aggaagataa aggaagagga gaagagggtc
2161 aacagcaagt ttgacaccat ttatcagatt ctcctcaaga agaagagtga gatccagacc
2221 ttgaaggagg agattgaaca gagcctgacc aagagggatg agttcgagtt tctggagaaa
2281 gcatcaaaac tgcgaggaat ctcaacaaag ccagtctaca tccccgaggt ggaactgaac
2341 cacaagctga taaaaggcat ccaccagagc accatagacc tcaaaaacga gctgaagcag
2401 tgcatcgggc ggctccagga gcccaccccc agttcaggtg accctggaga gcatgaccca
2461 gcgtccacac acaaatccac acgccctgtg aagaaggtct ccaaagagga aaagaaatcc
2521 aagaaacctc cccctgtccc tgccttaccc agcaagcttc ccacgtttgg agccccggaa
2581 cagttagtgg atttaaaaca agctggcttg gaggctgcag ccaaagccac cagctcacat
2641 ccgaactcaa catctctcaa ggccaaggtg ctggagacct tcctggccaa gtccagacct
2701 gagctcctgg agtattacat taaagtcatc ctggactaca acaccgccca caacaaagtg
2761 gctctgtcag agtgctatac agtagcttct gtggctgaga tgcctcagaa ctaccggccg
2821 catccccaga ggttcacata ctgctctcag gtgctgggcc tgcactgcta caagaagggg
2881 atccactact gggaggtgga gctgcagaag aacaacttct gtggggtagg catctgctac
2941 ggaagcatga accggcaggg cccagaaagc aggctcggcc gcaacagcgc ctcctggtgc
3001 gtggagtggt tcaacaccaa gatctctgcc tggcacaata acgtggagaa aaccctgccc
3061 tccaccaagg ccacgcgggt gggcgtgctt ctcaactgtg accacggctt tgtcatcttc
3121 ttcgctgttg ccgacaaggt ccacctgatg tataagttca gggtggactt tactgaggct
3181 ttgtacccgg ctttctgggt attttctgct ggtgccacac tctccatctg ctcccccaag
3241 taggaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
3301 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
3361 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
3421 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
3481 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
3541 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
3601 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
3661 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
3721 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
3781 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
3841 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
3901 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
3961 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
4021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4081 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
4141 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
4201 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
4261 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
4321 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
4381 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
4441 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
4501 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
4561 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
4621 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
4681 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
4741 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
4801 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
4861 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
4921 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
4981 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
5041 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
5101 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
5161 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
5221 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
5281 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
5341 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
5401 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
5461 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
5521 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
5581 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
5641 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
5701 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
5761 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
5821 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
5881 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
5941 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
6001 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
6061 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
6121 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
6181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
6241 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
6301 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
6361 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
6421 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
6481 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
6541 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
6601 tattaccgcc atgcat
//
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