pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYLK-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYLK-FLAG                6831 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3544
                     /codon_start=1
                     /label=MYLK
                     /translation="VRAGESVELFGKVTGTQPITCTWMKFRKQIQESEHMKVENSENGS
                     KLTILAARQEHCGCYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSW
                     YGSSYDGGSAVQSYSIEIWDSANKTWKELATCRSTSFNVQDLLPDHEYKFRVRAINVYG
                     TSEPSQESELTTVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERL
                     GSGKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFE
                     EKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKP
                     ENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSI
                     GVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKN
                     RLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMIS
                     GLSGRKSSTGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGS
                     AARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCK
                     AVNSLGEATCTAELIVETMEEGEGEGEEEEE"
     misc_feature    2428..2433
                     /label=SDS
     misc_feature    2630..2635
                     /label=SDS
     CDS             3551..3616
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3620..4051
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3764..3769
                     /label=SDS
     repeat_region   4094..4234
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4094..4223
                     /label=AAV2 ITR
     rep_origin      4309..4764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5046..5150
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5151..6011
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6182..6770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtac gcgcaggaga
     1561 gtcagtggag ctgtttggca aagtgacagg cactcagccc atcacctgta cctggatgaa
     1621 gttccgaaag cagatccagg aaagcgagca catgaaggtg gagaacagcg agaatggcag
     1681 caagctcacc atcctggccg cgcgccagga gcactgcggc tgctacacac tgctggtgga
     1741 gaacaagctg ggcagcaggc aggcccaggt caacctcact gtcgtggata agccagaccc
     1801 cccagctggc acaccttgtg cctctgacat tcggagctcc tcactgaccc tgtcctggta
     1861 tggctcctca tatgatgggg gcagtgctgt acagtcctac agcatcgaga tctgggactc
     1921 agccaacaag acgtggaagg aactagccac atgccgcagc acctctttca acgtccagga
     1981 cctgctgcct gaccacgaat ataagttccg tgtacgtgca atcaacgtgt atggaaccag
     2041 tgagccaagc caggagtctg aactcacaac ggtaggagag aaacctgaag agccgaagga
     2101 tgaagtggag gtgtcagatg atgatgagaa ggagcccgag gttgattacc ggacagtgac
     2161 aatcaatact gaacaaaaag tatctgactt ctacgacatt gaggagagat taggatctgg
     2221 gaaatttgga caggtctttc gacttgtaga aaagaaaact cgaaaagtct gggcagggaa
     2281 gttcttcaag gcatattcag caaaagagaa agagaatatc cggcaggaga ttagcatcat
     2341 gaactgcctc caccacccta agctggtcca gtgtgtggat gcctttgaag aaaaggccaa
     2401 catcgtcatg gtcctggaga tcgtgtcagg aggggagctg tttgagcgca tcattgacga
     2461 ggactttgag ctgacggagc gtgagtgcat caagtacatg cggcagatct cggagggagt
     2521 ggagtacatc cacaagcagg gcatcgtgca cctggacctc aagccggaga acatcatgtg
     2581 tgtcaacaag acgggcacca ggatcaagct catcgacttt ggtctggcca ggaggctgga
     2641 gaatgcgggg tctctgaagg tcctctttgg caccccagaa tttgtggctc ctgaagtgat
     2701 caactatgag cccatcggct acgccacaga catgtggagc atcggggtca tctgctacat
     2761 cctagtcagt ggcctttccc ccttcatggg agacaacgat aacgaaacct tggccaacgt
     2821 tacctcagcc acctgggact tcgacgacga ggcattcgat gagatctccg acgatgccaa
     2881 ggatttcatc agcaatctgc tgaagaaaga tatgaaaaac cgcctggact gcacgcagtg
     2941 ccttcagcat ccatggctaa tgaaagatac caagaacatg gaggccaaga aactctccaa
     3001 ggaccggatg aagaagtaca tggcaagaag gaaatggcag aaaacgggca atgctgtgag
     3061 agccattgga agactgtcct ctatggcaat gatctcaggg ctcagtggca ggaaatcctc
     3121 aacagggtca ccaaccagcc cgctcaatgc agaaaaacta gaatctgaag aagatgtgtc
     3181 ccaagctttc cttgaggctg ttgctgagga aaagcctcat gtaaaaccct atttctctaa
     3241 gaccattcgc gatttagaag ttgtggaggg aagtgctgct agatttgact gcaagattga
     3301 aggataccca gaccccgagg ttgtctggtt caaagatgac cagtcaatca gggagtcccg
     3361 ccacttccag atagactacg atgaggacgg gaactgctct ttaattatta gtgatgtttg
     3421 cggggatgac gatgccaagt acacctgcaa ggctgtcaac agtcttggag aagccacctg
     3481 cacagcagag ctcattgtgg aaacgatgga ggaaggtgaa ggggaagggg aagaggaaga
     3541 agagtgtaca gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     3601 ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat ttgtgaaaga
     3661 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3721 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3781 tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3841 gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt
     3901 gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3961 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     4021 ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg gcacgtgcgg
     4081 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     4141 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     4201 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     4261 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     4321 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4381 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4441 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4501 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     4561 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4621 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     4681 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4741 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     4801 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4861 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4921 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     4981 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5041 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5101 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5161 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5221 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5281 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5341 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5401 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5461 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5521 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5581 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5641 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5701 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5761 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5821 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5881 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5941 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6001 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6061 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6121 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6181 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6241 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6301 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6361 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6421 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6481 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6541 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6601 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6661 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6721 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6781 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//