| 质粒名称: | pAAVLINK-EFS-5'-PLXNA4 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001575 |
| 产品用途: | 5' AAVLINK plasmid for PLXNA4 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PLXNA4 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-5'-PLXNA4
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-5'-PLXNA4载体质粒基本信息
pAAVLINK-EFS-5'-PLXNA4质粒图谱载体图谱和pAAVLINK-EFS-5'-PLXNA4载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-5'-PLXNA4质粒载体简介
pAAVLINK-EFS-5'-PLXNA4质粒序列载体序列
LOCUS pAAVLINK-EFS-5'-PLXNA4 7313 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7313)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7313
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 357..4453
/codon_start=1
/label=PLXNA4
/translation="RTPP*KPCPGTGPAFSPTSSWWAWAPPLCSPGSQPRCPRSSGHLS
HSEESPPRVSITWWWMRGQDTFTWGPSIGFTSSPAT*RSW*RMRQGRTRTTPSVTHPAS
SRPAMSP*PPPTMSTRCSS*TTRRTG*LPVGACTKASASC*GWRTSSSWGSLIIRRSTI
CQVSTRAAQSLE*SSPTATWMTSCSLPRQWMGSPSIFPPSPAGN*PRTLRRMACSRTSS
MMSSWPR*LRSLRTPSPSSLTLISTMSMVLAVATLSTF*PSNLRWCLHQAPPPRSRCIH
PSS*GFARRTQPSTPM*RCPLAVSAVGWSTACCRLPTCPKRGPCLAGPLESIQMMTCSS
PSSPRARSGK*NPWMSRPCASSS*SR*MTALRSGCSLVTGARARWTWPGSR*RTSPAAV
RS*PLTITSVAWT*MLPWECPTWCVEFPSSRRTGTA*RLSSHMSTRTTLWPLWAPKVAS
*RRSGWMDPGATPSSMRRCRWWTPAQSSGIWPSPRTTSNSTSCQRGSSPESLWSPVVSI
RAAASALAQATPTVAGVCCTTLAPGRSGVSGPRSPAGLPRR*SSVSG*RSIPTISPSLS
TTCCWSWRRTMSRSCQLASTAPLRTCQRWMGWSWAIRSSATPLQPRRCPGSSQRMGTTM
SYSFSSNQRRPA*PSPAPALSSTIAASTIRACPAWRVHTAATGVNTGMSAPMTPRPAPS
RKAE*SCPRTAPSCCEWTRSWCPWR*SSLSR*RPRTSPSPSLGSVATNASSTFRAASSE
CPPCASTAPAYSARTPLIPMKGWRSTTCPWS*QSCGMGTSTLTTQLRIKFTSTSVEPCV
RAAGCASRLTQTSHVAGARAQASAPCASTALPRRASGWSCLVPKASAQTPASQR*SR*Q
APGKGAPRSLSEGRTWAWNFATSPPMSRLLAWSAAL*WMVTSLQNRSCVRWGRPSPASM
QASWRSAWLCVGLNSWPGPHSSITS*H*LSQI*SPAGGPCPEGPK*PSQAPT*MPEATW
W*CLESSPVSSTGDLHPTLSATPHPQMRC*R*RCRCRWTGPRSTRTWSFSMWKTPPSCG
LSQNGALSVETHPSPYGGPTWTSYRTPRSVPSMEGRSTSISVRF*TLLR*PVRRPPSLW
VLTTSQT*PRGPRSLASSWTTSSPCSSSTRPTSPTIPTRCLRPLVPQESWSSSLARPSS
*RART*SRLWLGAT*S*TTLCWLGRSRAP*PCQMSSCSASPPTSSAGTK*WPVSVAWST
PRGWCTLPRTARSACPPSSASQWLAASSSFSSWPCSLPINASPAKVTSR*SGCRCRWTT
WSPVWPWSARKPLPSCRRTSMS*PVTWMEPGFRSWTIELTPCGCCSQELKTTLSSGTLR
SRATGRSVWRKA*SSSPSSSTT"
misc_feature 409..414
/label=SDS
misc_feature 1221..1226
/label=SDS
misc_feature 2220..2225
/label=SDS
misc_feature 4108..4113
/label=SDS
intron 4454..4494
/label=Splicing Donor
misc_feature 4498..4531
/label=lox2272-JT1571
repeat_region 4576..4716
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4576..4705
/label=AAV2 ITR
rep_origin 4791..5246
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5528..5632
/gene="bla"
/label=AmpR promoter
CDS 5633..6493
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6664..7252
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cgccaccatg aaagccatgc cctggaactg gacctgcctt ctctcccacc tcctcatggt
421 gggcatgggc tcctccactt tgctcacccg gcagccagcc ccgctgtccc agaagcagcg
481 gtcatttgtc acattccgag gagagcccgc cgagggtttc aatcacctgg tggtggatga
541 gaggacagga cacatttact tgggggccgt caatcggatt tacaagctct ccagcgacct
601 gaaggtcttg gtgacgcatg agacagggcc ggacgaggac aaccccaagt gttacccacc
661 ccgcatcgtc cagacctgca atgagcccct gaccaccacc aacaatgtca acaagatgct
721 cctcatagac tacaaggaga acaggctgat tgcctgtggg agcctgtacc aaggcatctg
781 caagctgctg aggctggagg acctcttcaa gctgggggag ccttatcata agaaggagca
841 ctatctgtca ggtgtcaacg agagcggctc agtctttgga gtgatcgtct cctacagcaa
901 cctggatgac aagctgttca ttgccacggc agtggatggg aagcccgagt attttcccac
961 catctccagc cggaaactga ccaagaactc tgaggcggat ggcatgttcg cgtacgtctt
1021 ccatgatgag ttcgtggcct cgatgattaa gatcccttcg gacaccttca ccatcatccc
1081 tgactttgat atctactatg tctatggttt tagcagtggc aactttgtct actttttgac
1141 cctccaacct gagatggtgt ctccaccagg ctccaccacc aaggagcagg tgtatacatc
1201 caagctcgtg aggctttgca aggaggacac agccttcaac tcctatgtag aggtgcccat
1261 tggctgtgag cgcagtgggg tggagtaccg cctgctgcag gctgcctacc tgtccaaagc
1321 gggggccgtg cttggcagga cccttggagt ccatccagat gatgacctgc tcttcaccgt
1381 cttctccaag ggccagaagc ggaaaatgaa atccctggat gagtcggccc tgtgcatctt
1441 catcttgaag cagataaatg accgcattaa ggagcggctg cagtcttgtt accggggcga
1501 gggcacgctg gacctggcct ggctcaaggt gaaggacatc ccctgcagca gtgcgctctt
1561 aaccattgac gataacttct gtggcctgga catgaatgct cccctgggag tgtccgacat
1621 ggtgcgtgga attcccgtct tcacggagga cagggaccgc atgacgtctg tcatcgcata
1681 tgtctacaag aaccactctc tggcctttgt gggcaccaaa agtggcaagc tgaagaagat
1741 ccgggtggat ggacccaggg gcaacgccct ccagtatgag acggtgcagg tggtggaccc
1801 cggcccagtc ctccgggata tggccttctc caaggaccac gagcaactct acatcatgtc
1861 agagaggcag ctcaccagag tccctgtgga gtcctgtggt cagtatcaga gctgcggcga
1921 gtgccttggc tcaggcgacc cccactgtgg ctggtgtgtg ctgcacaaca cttgcacccg
1981 gaaggagcgg tgtgagcggt ccaaggagcc ccgcaggttt gcctcggaga tgaagcagtg
2041 tgtccggctg acggtccatc ccaacaatat ctccgtctct cagtacaacg tgctgctggt
2101 cctggagacg tacaatgtcc cggagctgtc agctggcgtc aactgcacct ttgaggacct
2161 gtcagagatg gatgggctgg tcgtgggcaa tcagatccag tgctactccc ctgcagccaa
2221 ggaggtgccc cggatcatca cagagaatgg ggaccaccat gtcgtacagc ttcagctcaa
2281 atcaaaggag accggcatga ccttcgccag caccagcttt gtcttctaca attgcagcgt
2341 ccacaattcg tgcctgtcct gcgtggagag tccataccgc tgccactggt gtaaataccg
2401 gcatgtctgc acccatgacc ccaagacctg ctccttccag gaaggccgag tgaagctgcc
2461 cgaggactgc ccccagctgc tgcgagtgga caagatcctg gtgcccgtgg aggtgatcaa
2521 gcctatcacg ctgaaggcca agaacctccc ccagccccag tctgggcagc gtggctacga
2581 atgcatcctc aacattcagg gcagcgagca gcgagtgccc gccctgcgct tcaacagctc
2641 cagcgtacag tgccagaaca cctcttattc ctatgaaggg atggagatca acaacctgcc
2701 cgtggagttg acagtcgtgt ggaatgggca cttcaacatt gacaacccag ctcagaataa
2761 agttcacctc tacaagtgtg gagccatgcg tgagagctgc gggctgtgcc tcaaggctga
2821 cccagacttc gcatgtggct ggtgccaggg cccaggccag tgcaccctgc gccagcactg
2881 ccctgcccag gagagccagt ggctggagct gtctggtgcc aaaagcaagt gcacaaaccc
2941 ccgcatcaca gagataatcc cggtgacagg cccccgggaa gggggcacca aggtcactat
3001 ccgaggggag aacctgggcc tggaatttcg cgacatcgcc tcccatgtca aggttgctgg
3061 cgtggagtgc agccctttag tggatggtta catccctgca gaacagatcg tgtgtgagat
3121 gggggaggcc aagcccagcc agcatgcagg cttcgtggag atctgcgtgg ctgtgtgtcg
3181 gcctgaattc atggcccggt cctcacagct ctattacttc atgacactga ctctctcaga
3241 tctgaagccc agccgggggc ccatgtccgg agggacccaa gtgaccatca caggcaccaa
3301 cctgaatgcc ggaagcaacg tggtggtgat gtttggaaag cagccctgtc tcttccacag
3361 gcgatctcca tcctacattg tctgcaacac cacatcctca gatgaggtgc tagagatgaa
3421 ggtgtcggtg caggtggaca gggccaagat ccaccaggac ctggtctttc agtatgtgga
3481 agaccccacc atcgtgcgga ttgagccaga atggagcatt gtcagtggaa acacacccat
3541 cgccgtatgg gggacccacc tggacctcat acagaacccc cagatccgtg ccaagcatgg
3601 agggaaggag cacatcaata tctgtgaggt tctgaacgct actgagatga cctgtcaggc
3661 gcccgccctc gctctgggtc ctgaccacca gtcagacctg accgagaggc ccgaggagtt
3721 tggcttcatc ctggacaacg tccagtccct gctcatcctc aacaagacca acttcaccta
3781 ctatcccaac ccggtgtttg aggcctttgg tccctcagga atcctggagc tcaagcctgg
3841 cacgcccatc atcctaaagg gcaagaacct gatcccgcct gtggctgggg gcaacgtgaa
3901 gctgaactac actgtgctgg ttggggagaa gccgtgcacc gtgaccgtgt cagatgtcca
3961 gctgctctgc gagtccccca acctcatcgg caggcacaaa gtgatggccc gtgtcggtgg
4021 catggagtac tccccgggga tggtgtacat tgccccggac agcccgctca gcctgcccgc
4081 catcgtcagc atcgcagtgg ctggcggcct cctcatcatt ttcatcgtgg ccgtgctcat
4141 tgcctataaa cgcaagtccc gcgaaagtga cctcacgctg aagcggctgc agatgcagat
4201 ggacaacctg gagtcccgtg tggccctgga gtgcaaggaa gcctttgccg agctgcagac
4261 ggacatccat gagctgacca gtgacctgga tggagccggg attccgttcc tggactatag
4321 aacttacacc atgcgggtgc tgttcccagg aattgaagac caccctgtcc tccgggacct
4381 tgaggtcccg ggctaccggc aggagcgtgt ggagaaaggc ctgaagctct tcgcccagct
4441 catcaacaac aaggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat
4501 tattcgtata ggatacttta tacgaagtta ttaagttatg gagaccggtg tggcacgtgc
4561 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
4621 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
4681 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
4741 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
4801 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
4861 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4921 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4981 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
5041 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
5101 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
5161 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
5221 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
5281 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
5341 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
5401 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
5461 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
5521 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
5581 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
5641 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
5701 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
5761 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
5821 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
5881 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
5941 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
6001 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
6061 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
6121 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
6181 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
6241 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
6301 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
6361 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
6421 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
6481 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
6541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
6601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
6661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
6721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
6781 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
6841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
6901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
6961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
7021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
7081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
7141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
7201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
7261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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pAAVLINK-EFS-5'-PLXNA4质粒载体应用举例
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