pMXs-puro-hMGT

LOCUS       pMXs-puro-hMGT      10055 bp    DNA     circular SYN 04-SEP-2026
DEFINITION  Retroviral expression vector for human direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Zhou Y, Liu Z, Welch JD, Gao X, Wang L, Garbutt T, Keepers B, Ma H, 
            Prins JF, Shen W, Liu J, Qian L
  TITLE     Single-Cell Transcriptomic Analyses of Cell Fate Transitions during 
            Human Cardiac Reprogramming.
  JOURNAL   Cell Stem Cell. 2019 Jul 3;25(1):149-164.e9. doi: 
            10.1016/j.stem.2019.05.020. Epub 2019 Jun 20.
  PUBMED    31230860
REFERENCE   2  (bases 1 to 10055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 4, 2026 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.05.020"; journalName: "Cell Stem Cell"; date: 
            "2019-07-3- 3"; volume: "25"; issue: "1"; pages: "149-164.e9"
COMMENT     Sequence Label: pMXs-puro-hMGT
FEATURES             Location/Qualifiers
     source          1..10055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1073..1489
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1419..1441
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1457..1473
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1499..1873
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1811..1827
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             3324..3380
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4728..4781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6369..6389)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6374..6703
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6554..6689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6713..7312
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6713..6732)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7209..7229
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7539..8008
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8138..8155)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8309..8897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8389..8408)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9061..9852,9853..9921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9684..9703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9922..10026)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
      901 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     1081 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1141 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1201 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1261 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1321 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1381 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1441 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     1501 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     1561 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     1621 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     1681 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     1741 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     1801 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     1861 atcctctaga ctgccggatc tagctagtta attaaggatc cgttaacatc gataccgcca
     1921 tggggagaaa aaagattcag attacgagga ttatggatga acgtaacaga caggtgacat
     1981 ttacaaagag gaaatttggg ttgatgaaga aggcttatga gctgagcgtg ctgtgtgact
     2041 gtgagattgc gctgatcatc ttcaacagca ccaacaagct gttccagtat gccagcaccg
     2101 acatggacaa agtgcttctc aagtacacgg agtacaacga gccgcatgag agccggacaa
     2161 actcagacat cgtggaggca ttgaacaaga aagaaaacaa aggctgtgaa agccccgatc
     2221 ccgactcctc ttatgcactc accccacgca ctgaagaaaa atacaaaaaa attaatgaag
     2281 aatttgataa tatgatcaag agtcataaaa ttcctgctgt tccacctccc aacttcgaga
     2341 tgccagtctc catcccagtg tccagccaca acagtttggt gtacagcaac cctgtcagct
     2401 cactgggaaa ccccaaccta ttgccactgg ctcacccttc tctgcagagg aatagtatgt
     2461 ctcctggtgt aacacatcga cctccaagtg caggtaacac aggtggtctg atgggtggag
     2521 acctcacgtc tggtgcaggc accagtgcag ggaacgggta tggcaatccc cgaaactcac
     2581 caggtctgct ggtctcacct ggtaacttga acaagaatat gcaagcaaaa tctcctcccc
     2641 caatgaattt aggaatgaat aaccgtaaac cagatctccg agttcttatt ccaccaggca
     2701 gcaagaatac gatgccatca gtgaatcaaa ggataaataa ctcccagtcg gctcagtcat
     2761 tggctacccc agtggtttcc gtagcaactc ctactttacc aggacaagga atgggaggat
     2821 atccatcagc catttcaaca acatatggta ccgagtactc tctgagtagt gcagacctgt
     2881 catctctgtc tgggtttaac accgccagcg ctcttcacct tggttcagta actggctggc
     2941 aacagcaaca cctacataac atgccaccat ctgccctcag tcagttggga gcttgcacta
     3001 gcactcattt atctcagagt tcaaatctct ccctgccttc tactcaaagc ctcaacatca
     3061 agtcagaacc tgtttctcct cctagagacc gtaccaccac cccttcgaga tacccacaac
     3121 acacgcgcca cgaggcgggg agatctcctg ttgacagctt gagcagctgt agcagttcgt
     3181 acgacgggag cgaccgagag gatcaccgga acgaattcca ctcccccatt ggactcacca
     3241 gaccttcgcc ggacgaaagg gaaagtccct cagtcaagcg catgcgactt tctgaaggat
     3301 gggcaacagc tagcggcagc ggcgccacaa acttctctct gctaaagcaa gcaggtgatg
     3361 ttgaagaaaa ccccgggcct actagtatgt atcagagctt ggccatggcc gccaaccacg
     3421 ggccgccccc cggtgcctac gaggcgggcg gccccggcgc cttcatgcac ggcgcgggcg
     3481 ccgcgtcctc gccagtctac gtgcccacac cgcgggtgcc ctcctccgtg ctgggcctgt
     3541 cctacctcca gggcggaggc gcgggctctg cgtccggagg cgcctcgggc ggcagctccg
     3601 gtggggccgc gtctggtgcg gggcccggga cccagcaggg cagcccggga tggagccagg
     3661 cgggagccga cggagccgct tacaccccgc cgccggtgtc gccgcgcttc tccttcccgg
     3721 ggaccaccgg gtccctggcg gccgccgccg ccgctgccgc ggcccgggaa gctgcggcct
     3781 acagcagtgg cggcggagcg gcgggtgcgg gcctggcggg ccgcgagcag tacgggcgcg
     3841 ccggcttcgc gggctcctac tccagcccct acccggctta catggccgac gtgggcgcgt
     3901 cctgggccgc agccgccgcc gcctccgccg gccccttcga cagcccggtc ctgcacagcc
     3961 tgcccggccg ggccaacccg gccgcccgac accccaatct cgatatgttt gacgacttct
     4021 cagaaggcag agagtgtgtc aactgtgggg ctatgtccac cccgctctgg aggcgagatg
     4081 ggacgggtca ctatctgtgc aacgcctgcg gcctctacca caagatgaac ggcatcaacc
     4141 ggccgctcat caagcctcag cgccggctgt ccgcctcccg ccgagtgggc ctctcctgtg
     4201 ccaactgcca gaccaccacc accacgctgt ggcgccgcaa tgcggagggc gagcctgtgt
     4261 gcaatgcctg cggcctctac atgaagctcc acggggtccc caggcctctt gcaatgcgga
     4321 aagaggggat ccaaaccaga aaacggaagc ccaagaacct gaataaatct aagacaccag
     4381 cagctccttc aggcagtgag agccttcctc ccgccagcgg tgcttccagc aactccagca
     4441 acgccaccac cagcagcagc gaggagatgc gtcccatcaa gacggagcct ggcctgtcat
     4501 ctcactacgg gcacagcagc tccgtgtccc agacgttctc agtcagtgcg atgtctggcc
     4561 atgggccctc catccaccct gtcctctcgg ccctgaagct ctccccacaa ggctatgcgt
     4621 ctcccgtcag ccagtctcca cagaccagct ccaagcagga ctcttggaac agcctggtct
     4681 tggccgacag tcacggggac ataatcactg cgaagcttgg ctccggcgag ggcaggggaa
     4741 gtcttctaac atgcggggac gtggaggaaa atcccggccc actcgacatg gccgacgcag
     4801 acgagggctt tggcctggcg cacacgcctc tggagcctga cgcaaaagac ctgccctgcg
     4861 attcgaaacc cgagagcgcg ctcggggccc ccagcaagtc cccgtcgtcc ccgcaggccg
     4921 ccttcaccca gcagggcatg gagggaatca aagtgtttct ccatgaaaga gaactgtggc
     4981 taaaattcca cgaagtgggc acggaaatga tcataaccaa ggctggaagg cggatgtttc
     5041 ccagttacaa agtgaaggtg acgggcctta atcccaaaac gaagtacatt cttctcatgg
     5101 acattgtacc tgccgacgat cacagataca aattcgcaga taataaatgg tctgtgacgg
     5161 gcaaagctga gcccgccatg cctggccgcc tgtacgtgca cccagactcc cccgccaccg
     5221 gggcgcattg gatgaggcag ctcgtctcct tccagaaact caagctcacc aacaaccacc
     5281 tggacccatt tgggcatatt attctaaatt ccatgcacaa ataccagcct agattacaca
     5341 tcgtgaaagc ggatgaaaat aatggatttg gctcaaaaaa tacagcgttc tgcactcacg
     5401 tctttcctga gactgcgttt atagcagtga cttcctacca gaaccacaag atcacgcaat
     5461 taaagattga gaataatccc tttgccaaag gatttcgggg cagtgatgac atggagctgc
     5521 acagaatgtc aagaatgcaa agtaaagaat atcccgtggt ccccaggagc accgtgaggc
     5581 aaaaagtggc ctccaaccac agtcctttca gcagcgagtc tcgagctctc tccacctcat
     5641 ccaatttggg gtcccaatac cagtgtgaga atggtgtttc cggcccctcc caggacctcc
     5701 tgcctccacc caacccatac ccactgcccc aggagcatag ccaaatttac cattgtacca
     5761 agaggaaaga ggaagaatgt tccaccacag accatcccta taagaagccc tacatggaga
     5821 catcacccag tgaagaagat tccttctacc gctctagcta tccacagcag cagggcctgg
     5881 gtgcctccta caggacagag tcggcacagc ggcaagcttg catgtatgcc agctctgcgc
     5941 cccccagcga gcctgtgccc agcctagagg acatcagctg caacacgtgg ccaagcatgc
     6001 cttcctacag cagctgcacc gtcaccaccg tgcagcccat ggacaggcta ccctaccagc
     6061 acttctccgc tcacttcacc tcggggcccc tggtccctcg gctggctggc atggccaacc
     6121 atggctcccc acagctggga gagggaatgt tccagcacca gacctccgtg gcccaccagc
     6181 ctgtggtcag gcagtgtggg cctcagactg gcctgcagtc ccctggcacc cttcagcccc
     6241 ctgagttcct ctactctcat ggcgtgccaa ggactctatc ccctcatcag taccactctg
     6301 tgcacggagt tggcatggtg ccagagtgga gcgacaatag ctaataacca gcacagtggt
     6361 cgacctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6421 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6481 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6541 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6601 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6661 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga
     6721 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6781 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6841 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6901 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6961 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7021 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7081 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7141 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7201 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7261 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     7321 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgt cgacgataaa
     7381 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     7441 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
     7501 agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
     7561 atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata
     7621 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     7681 tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg
     7741 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     7801 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     7861 tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
     7921 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     7981 ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt
     8041 ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg
     8101 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8161 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8221 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8281 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8341 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8401 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8461 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8521 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8581 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8641 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8701 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8761 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8821 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8881 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8941 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9001 tccttttgcg gccggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9061 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9121 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9181 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9241 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9301 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9361 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9421 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9481 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9541 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9601 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9661 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9721 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9781 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9901 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9961 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10021 tccgcgcaca tttccccgaa aagtgccacc tgcat
//