| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001577 |
| 产品用途: | 3' AAVLINK plamid for PLXNA4 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PLXNA4 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG 6441 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6441)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6441
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3142
/codon_start=1
/label=PLXNA4
/translation="VFLLSFIRTLESQRSFSMRDRGNVASLIMTVLQSKLEYATDVLKQ
LLADLIDKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLYKFLKECAGEPLFSLFCAIK
QQMEKGPIDAITGEARYSLSEDKLIRQQIDYKTLVLSCVSPDNANSPEVPVKILNCDTI
TQVKEKILDAIFKNVPCSHRPKAADMDLEWRQGSGARMILQDEDITTKIENDWKRLNTL
AHYQVPDGSVVALVSKQVTAYNAVNNSTVSRTSASKYENMIRYTGSPDSLRSRTPMITP
DLESGVKMWHLVKNHEHGDQKEGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETIFST
AHRGSALPLAIKYMFDFLDEQADKHGIHDPHVRHTWKSNCLPLRFWVNMIKNPQFVFDI
HKNSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPSYKNWVERYYSDIGK
MPAISDQDMNAYLAEQSRMHMNEFNTMSALSEIFSYVGKYSEEILGPLDHDDQCGKQKL
AYKLEQVITLMSLDS"
misc_feature 1742..1747
/label=SDS
misc_feature 1792..1797
/label=SDS
misc_feature 2451..2456
/label=SDS
CDS 3149..3214
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3230..3661
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3374..3379
/label=SDS
repeat_region 3704..3844
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3704..3833
/label=AAV2 ITR
rep_origin 3919..4374
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4656..4760
/gene="bla"
/label=AmpR promoter
CDS 4761..5621
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5792..6380
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt tcctgctgtc
1561 cttcatccgc acgcttgagt cccagcgtag cttctccatg cgcgaccgtg gcaacgtggc
1621 ctcactcatc atgaccgtgc tgcagagcaa gctggagtac gccactgatg tgctgaagca
1681 gctgctggcc gacctcattg acaagaacct ggagagcaag aaccacccta agctgctgct
1741 caggaggact gagtcagtgg ctgagaagat gctgaccaat tggtttactt tcctcctcta
1801 caagttcctc aaggagtgtg ctggggagcc cctcttctcc ctgttctgtg ccatcaagca
1861 gcagatggag aagggcccca ttgacgccat cacgggcgag gcccgctact ccttgagcga
1921 ggacaagctc atccgccagc agattgacta caaaaccctg gtcctgagct gtgtcagccc
1981 agacaatgcc aacagccccg aggtcccagt aaagatcctc aactgtgaca ccatcactca
2041 ggtcaaggag aagattctgg atgccatctt caagaatgtg ccttgctccc accggcccaa
2101 agctgcagat atggatctgg agtggcgaca aggaagtggg gcaaggatga tcttgcagga
2161 tgaagacatc accaccaaga ttgagaatga ttggaagcga ctgaacacac tggcccacta
2221 ccaggtgcca gatggttccg tggtggcatt agtgtccaag caggtgacag cctataacgc
2281 agtgaacaac tccaccgtct ccaggacctc agcaagtaaa tatgaaaaca tgatccggta
2341 cacgggcagc cccgacagcc tccgctcacg gacacctatg atcactcctg acctggagag
2401 tggagtcaag atgtggcacc tagtgaagaa ccacgagcac ggagaccaga aggaggggga
2461 ccgggggagc aagatggtgt ctgaaatcta cctgacccga ctcctggcca ctaagggcac
2521 actgcagaag tttgtggatg acctctttga gaccatcttc agcacggcac accgtggctc
2581 tgccctgccc ctggccatca agtacatgtt tgacttcctg gatgagcagg ctgataaaca
2641 tggcattcat gacccgcacg tccgccatac ctggaagagc aattgcctgc ccctgaggtt
2701 ttgggtcaac atgatcaaga acccgcagtt tgtgtttgac atccataaga acagcatcac
2761 agacgcctgc ctctctgtgg tggctcagac cttcatggac tcttgctcca cgtcagagca
2821 ccggctgggc aaggactcgc cctccaacaa gctgctgtat gccaaggaca tccccagcta
2881 caagaattgg gtggagaggt attactcaga catagggaag atgccagcca tcagcgacca
2941 agacatgaac gcatacctgg ctgagcagtc ccggatgcac atgaatgagt tcaacaccat
3001 gagtgcgctc tcagagatct tctcctatgt gggcaaatac agcgaggaga tccttggacc
3061 tctggaccac gacgaccagt gtgggaagca gaaactggcc tacaaactag aacaagtcat
3121 aaccctcatg agcttagaca gctgtacaga ctacaaagac catgacggtg attataaaga
3181 tcatgacatc gactacaagg atgacgatga caagtaagaa ttctgatgac tcgagataat
3241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
3301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
3361 gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc
3421 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
3481 gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg
3541 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
3601 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
3661 aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
3721 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
3781 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
3841 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
3901 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
3961 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
4021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
4081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
4141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
4201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
4261 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
4321 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
4381 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
4441 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
4501 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
4561 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
4621 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
4681 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
4741 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
4801 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
4861 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
4921 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4981 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
5041 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
5101 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
5161 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
5221 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
5281 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
5341 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
5401 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
5461 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
5521 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
5581 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
5641 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
5701 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
5761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
5821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
5881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
6001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
6061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
6121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
6181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
6241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
6301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
6361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
6421 gctggccttt tgctcacatg t
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PLXNA4-FLAG质粒载体应用举例
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