pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ADGRL1-HA

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ADGRL1-HA              7353 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..4036
                     /codon_start=1
                     /label=ADGRL1
                     /translation="VHTATMLLDVLEEGAFLLADNVREPARFLAAKENVVLEVTVLNTE
                     GQVQELVFPQEEYPRKNSIQLSAKTIKQNSRNGVVKVVFILYNNLGLFLSTENATVKLA
                     GEAGPGGPGGASLVVNSQVIAASINKESSRVFLMDPVIFTVAHLEDKNHFNANCSFWNY
                     SERSMLGYWSTQGCRLVESNKTHTTCACSHLTNFAVLMAHREIYQGRINELLLSVITWV
                     GIVISLVCLAICISTFCFLRGLQTDRNTIHKNLCINLFLAELLFLVGIDKTQYEIACPI
                     FAGLLHYFFLAAFSWLCLEGVHLYLLLVEVFESEYSRTKYYYLGGYCFPALVVGIAAAI
                     DYRSYGTEKACWLRVDNYFIWSFIGPVSFVIVVNLVFLMVTLHKMIRSSSVLKPDSSRL
                     DNIKSWALGAIALLFLLGLTWAFGLLFINKESVVMAYLFTTFNAFQGVFIFVFHCALQK
                     KVHKEYSKCLRHSYCCIRSPPGGTHGSLKTSAMRSNTRYYTGTQSRIRRMWNDTVRKQT
                     ESSFMAGDINSTPTLNRGTMGNHLLTNPVLQPRGGTSPYNTLIAESVGFNPSSPPVFNS
                     PGSYREPKHPLGGREACGMDTLPLNGNFNNSYSLRSGDFPPGDGGPEPPRGRNLADAAA
                     FEKMIISELVHNNLRGSSSAAKGPPPPEPPVPPVPGGGGEEEAGGPGGADRAEIELLYK
                     ALEEPLLLPRAQSVLYQSDLDESESCTAEDGATSRPLSSPPGRDSLYASGANLRDSPSY
                     PDSSPEGPSEALPPPPPAPPGPPEIYYTSRPPALVARNPLQGYYQVRRPSHEGYLAAPG
                     LEGPGPDGDGQMQLVTSL"
     misc_feature    1581..1586
                     /label=SDS
     misc_feature    1710..1715
                     /label=SDS
     misc_feature    2815..2820
                     /label=SDS
     misc_feature    3102..3107
                     /label=SDS
     misc_feature    3249..3254
                     /label=SDS
     misc_feature    3746..3751
                     /label=SDS
     misc_feature    3798..3803
                     /label=SDS
     CDS             4052..4078
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4082..4108
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4112..4136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDY"
     terminator      4142..4573
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4286..4291
                     /label=SDS
     repeat_region   4616..4756
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4616..4745
                     /label=AAV2 ITR
     rep_origin      4831..5286
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5568..5672
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5673..6533
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6704..7292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgc acacggccac
     1561 catgctcctc gacgtcctgg aggagggcgc cttcctgctg gccgacaatg tcagggagcc
     1621 tgcccgcttc ctggctgcca aggagaacgt ggtcctggag gtcacagtcc tgaacacaga
     1681 gggccaggtg caggagctgg tgttccccca ggaggagtac ccgagaaaga actccatcca
     1741 gctgtctgcc aaaaccatca agcagaacag ccgcaatggg gtggtcaaag ttgtcttcat
     1801 cctctacaac aacctgggcc tcttcctgtc cacggagaat gccacagtga agctggccgg
     1861 cgaagcaggc ccgggtggcc ctgggggcgc ctctctagtg gtgaactcac aggtcatcgc
     1921 agcatccatc aacaaggagt ccagccgcgt cttcctcatg gaccctgtca tcttcaccgt
     1981 ggcccacctg gaggacaaga accacttcaa tgctaactgc tccttctgga actactcgga
     2041 gcgttccatg ctgggctact ggtcgaccca aggctgccgc ctggtggagt ccaacaagac
     2101 ccataccacg tgtgcctgca gccacctcac caacttcgct gtgctcatgg ctcaccgtga
     2161 gatctaccag ggccgcatca acgagctgct gctgtcggtc atcacctggg tgggcattgt
     2221 gatctccctg gtctgcttgg ccatctgcat ctccaccttc tgcttcctgc gggggctgca
     2281 gaccgaccgc aacaccatcc acaagaacct gtgcatcaac ctcttcctgg ctgagctgct
     2341 cttcctggtc gggatcgaca agactcagta tgagattgcc tgccccatct tcgccggcct
     2401 gctgcactat ttcttcctgg ctgccttctc ctggctgtgc ctggagggcg tgcacctcta
     2461 cctgctacta gtggaggtgt ttgagagcga gtattcccgc accaagtact actacctggg
     2521 tggctactgc ttcccggccc tggtggtggg catcgcggct gccattgact accgcagcta
     2581 cggcaccgag aaggcctgct ggctccgagt ggacaattac ttcatctgga gtttcatcgg
     2641 gccagtctcc ttcgttatcg tggtcaacct ggtgttcctc atggtgaccc tgcacaagat
     2701 gatccgaagc tcatctgtgc tcaagcccga ctccagccgc ctggacaaca ttaaatcctg
     2761 ggcgctgggg gccatcgcgc tgctgttcct gctgggcctc acctgggctt tcggcctcct
     2821 cttcatcaac aaggagtcgg tggtcatggc ctatctcttc accaccttca acgccttcca
     2881 gggggtcttc atcttcgtct ttcactgcgc cttacagaag aaggtgcaca aggagtacag
     2941 caagtgcctg cgtcactcct actgctgcat ccgctcccca cccgggggca ctcacggatc
     3001 cctcaagacc tcagccatgc gaagcaacac ccgctactac acagggaccc agagccgaat
     3061 tcggaggatg tggaatgaca ctgtgaggaa acagacggag tcctccttca tggcgggtga
     3121 catcaacagc acccccaccc tgaaccgagg taccatgggg aaccacctgc tgaccaaccc
     3181 cgtgctgcag ccccgtgggg gcaccagtcc ctacaacacc ctcatcgccg agtcagtggg
     3241 cttcaatccc tcctcgcccc ctgtcttcaa ctccccaggg agctaccggg aacccaagca
     3301 ccccttggga ggccgggaag cctgtggcat ggacaccctg cccctgaacg gcaacttcaa
     3361 taacagttac tccttgcgaa gtggggattt ccctcccggg gatgggggcc ctgagccgcc
     3421 ccgaggccgg aacctagccg atgcggcggc ctttgagaag atgatcatct cagagctggt
     3481 gcacaacaac ctgcggggga gcagcagcgc ggccaagggc cctccaccgc ctgagccccc
     3541 tgtgccacct gtgccagggg gcgggggcga ggaagaggcg ggcgggcccg ggggtgctga
     3601 ccgggccgag attgaacttc tctataaggc cctggaggag cctctgctgc tgccccgggc
     3661 ccagtcggtg ctgtaccaga gcgatctgga cgagtcggag agctgcacgg ccgaggacgg
     3721 cgccaccagc cggcccctct cctcccctcc tggccgggac tccctctatg ccagcggggc
     3781 caacctgcgg gactcaccct cctacccgga cagcagccct gaggggccca gtgaggccct
     3841 gcccccaccc cctcccgcac cccccggccc ccccgaaatc tactacacct cgcgcccgcc
     3901 agccctggtg gcccggaatc ccctgcaggg ctactaccag gtgcggcgtc ctagccacga
     3961 gggctacctg gcagccccag gccttgaggg gccagggccc gatggggacg ggcagatgca
     4021 gctggtcacc agtctctgta caggcggaag ctacccgtat gatgttccgg attacgctgg
     4081 ctatccctac gacgtgcccg actatgccgg gtacccctat gacgtcccag actacgcata
     4141 actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4201 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4261 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
     4321 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4381 gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
     4441 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4501 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     4561 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
     4621 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4681 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4741 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4801 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     4861 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     4921 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4981 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5041 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5101 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5161 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     5221 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     5281 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5341 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5401 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5461 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5521 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5581 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5641 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5701 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5761 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5821 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5881 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5941 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6001 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6061 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6121 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6181 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6241 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6301 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6361 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6421 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6481 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6541 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6601 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6661 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6721 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6781 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6841 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6901 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6961 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7021 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7081 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7141 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7201 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7261 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7321 tcctggcctt ttgctggcct tttgctcaca tgt
//