LOCUS       pFUSE-hIgG1e2-Fc        4132 bp    DNA     circular SYN 31-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   DotmaticsRD
  TITLE     Direct Submission
  JOURNAL   Exported Jul 31, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..242
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             255..523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
                     /note="color: #ffe4c4"
     polyA_signal    1297..1418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_signal    1522..1916
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(1930..2304)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        2897..3200
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     rep_origin      complement(3280..3868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     polyA_signal    3976..4024
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    4035..4126
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
ORIGIN
        1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
       61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
      121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
      301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
      361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
      421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
      481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
      541 ctacctgaga tcaccggtga attcgatatc tcgagcacca tggttagatc tgacaaaact
      601 cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt cttcctcttc
      661 cccccaaaac ccaaggacac cctctacatc acccgggaac ctgaggtcac atgcgtggtg
      721 gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag
      781 gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc
      841 agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc
      901 tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc
      961 cgagaaccac aggtgtacac cctgccccca tcccgggagg agatgaccaa gaaccaggtc
     1021 agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc
     1081 aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc
     1141 ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc
     1201 tcatgctccg tgatgcatga ggctctgaag ttccactaca cgcagaagag cctctccctg
     1261 tctccgggta aatgagtgct agctggccag acatgataag atacattgat gagtttggac
     1321 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1381 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1441 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1501 aatgtggtat ggaattaatt ctaaaataca gcatagcaaa actttaacct ccaaatcaag
     1561 cctctacttg aatccttttc tgagggatga ataaggcata ggcatcaggg gctgttgcca
     1621 atgtgcatta gctgtttgca gcctcacctt ctttcatgga gtttaagata tagtgtattt
     1681 tcccaaggtt tgaactagct cttcatttct ttatgtttta aatgcactga cctcccacat
     1741 tcccttttta gtaaaatatt cagaaataat ttaaatacat cattgcaatg aaaataaatg
     1801 ttttttatta ggcagaatcc agatgctcaa ggcccttcat aatatccccc agtttagtag
     1861 ttggacttag ggaacaaagg aacctttaat agaaattgga cagcaagaaa gcgagcttct
     1921 agcttatcct cagtcctgct cctctgccac aaagtgcacg cagttgccgg ccgggtcgcg
     1981 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
     2041 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
     2101 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
     2161 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
     2221 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
     2281 aacggcactg gtcaacttgg ccatgatggc tcctcctgtc aggagaggaa agagaagaag
     2341 gttagtacaa ttgctatagt gagttgtatt atactatgca gatatactat gccaatgatt
     2401 aattgtcaaa ctagggctgc agggttcata gtgccacttt tcctgcactg ccccatctcc
     2461 tgcccaccct ttcccaggca tagacagtca gtgacttacc aaactcacag gagggagaag
     2521 gcagaagctt gagacagacc cgcgggaccg ccgaactgcg aggggacgtg gctagggcgg
     2581 cttcttttat ggtgcgccgg ccctcggagg cagggcgctc ggggaggcct agcggccaat
     2641 ctgcggtggc aggaggcggg gccgaaggcc gtgcctgacc aatccggagc acataggagt
     2701 ctcagccccc cgccccaaag caaggggaag tcacgcgcct gtagcgccag cgtgttgtga
     2761 aatgggggct tgggggggtt ggggccctga ctagtcaaaa caaactccca ttgacgtcaa
     2821 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     2881 gccaaaaccg catcatcatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     2941 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     3001 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     3061 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     3121 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     3181 ttaccgtaag ttatgtaacg cctgcaggtt aattaagaac atgtgagcaa aaggccagca
     3241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3721 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atggctagtt aattaacatt
     3961 taaatcagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     4021 tgtgaatcgt aactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
     4081 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg aa
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