PCDNA3-myc-DNMT1

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联系方式:I47-825O-882O

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质粒基本信息

质粒名称: PCDNA3-myc-DNMT1 
出品公司: 爱迪基因
目录编号: 36939
存储实验室: Arthur Riggs

载体骨架基本信息
载体名称: pcDNA3
载体出品公司: Invitrogen
载体抗性: 氨苄霉素
空载体大小: 5400 bp
完整质粒大小: 10251 bp
载体修饰: pcDNA3/Myc was constructed by Zhaoxia Chen (see Comments section for details). DNMT1 was cloned into the pcDNA3/Myc vector.
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: DNMT1
物种来源: 人源
插入大小: 4851 bp
GenBank编号 X63692
启动子 CMV
标签/融合蛋白: Myc (N terminal on backbone)
克隆方法: 限制性内切酶
5' 克隆位点: EcoRI(未破坏)
3' 克隆位点: NotI (未破坏)
5' 测序引物 CMV-F
3' 测序引物 BGH-rev

文献引用方法
材料和方法部分: pcDNA3/Myc-DNMT1 was a gift from Arthur Riggs (爱迪基因 plasmid # 36939)
参考文献部分: The histone methyltransferase SETDB1 and the DNA methyltransferase DNMT3A interact directly and localize to promoters silenced in cancer cells. Li H, Rauch T, Chen ZX, Szabo PE, Riggs AD, Pfeifer GP. J Biol Chem. 2006 Jul 14;281(28):19489-500. Epub 2006 May 8. 10.1074/jbc.M513249200 PubMed 16682412 

质粒图谱信息

PCDNA3-myc-DNMT1质粒图谱

质粒简介

质粒序列

LOCUS       pcDNA3/Myc-DNMT1               10297 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  Mammalian expression of human DNMT1 with myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3/Myc-DNMT1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Li H, Rauch T, Chen ZX, Szabo PE, Riggs AD, Pfeifer GP
  TITLE     The histone methyltransferase SETDB1 and the DNA methyltransferase 
            DNMT3A interact directly and localize to promoters silenced in 
            cancer cells.
  JOURNAL   J Biol Chem. 2006 Jul 14;281(28):19489-500. Epub 2006 May 8.
  PUBMED    16682412
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|PCDNA3-myc-DNMT1|
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      895..904
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             904..933
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(5850..5868)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5851..5868)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5888..5905)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5894..6118
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6164..6592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6251..6270)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6461..6482
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6601..6621)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6606..6935
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6786..6921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6848..6867
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7002..7796
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7056..7075)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7666..7685
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7970..8091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8007..8026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8061..8080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8140..8156)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8140..8156)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8153..8175)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8164..8180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8188..8218)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8233..8254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8371..8388)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8542..9130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8622..8641)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9301..10161)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9924..9943
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10162..10266)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atggagcaga agctgatctc agaggaggac ctggaattca tgccggcgcg taccgcccca
      961 gcccgggtgc ccacactggc cgtcccggcc atctcgctgc ccgacgatgt ccgcaggcgg
     1021 ctcaaagatt tggaaagaga cagcttaaca gaaaaggaat gtgtgaagga gaaattgaat
     1081 ctcttgcacg aatttctgca aacagaaata aagaatcagt tatgtgactt ggaaaccaaa
     1141 ttacgtaaag aagaattatc cgaggagggc tacctggcta aagtcaaatc ccttttaaat
     1201 aaagatttgt ccttggagaa cggtgctcat gcttacaacc gggaagtgaa tggacgtcta
     1261 gaaaacggga accaagcaag aagtgaagcc cgtagagtgg gaatggcaga tgccaacagc
     1321 ccccccaaac ccctttccaa acctcgcacg cccaggagga gcaagtccga tggagaggct
     1381 aagcctgaac cttcacctag ccccaggatt acaaggaaaa gcaccaggca aaccaccatc
     1441 acatctcatt ttgcaaaggg ccctgccaaa cggaaacctc aggaagagtc tgaaagagcc
     1501 aaatcggatg agtccatcaa ggaagaagac aaagaccagg atgagaagag acgtagagtt
     1561 acatccagag aacgagttgc tagaccgctt cctgcagaag aacctgaaag agcaaaatca
     1621 ggaacgcgca ctgaaaagga agaagaaaga gatgaaaaag aagaaaagag actccgaagt
     1681 caaaccaaag aaccaacacc caaacagaaa ctgaaggagg agccggacag agaagccagg
     1741 gcaggcgtgc aggctgacga ggacgaagat ggagacgaga aagatgagaa gaagcacaga
     1801 agtcaaccca aagatctagc tgccaaacgg aggcccgaag aaaaagaacc tgaaaaagta
     1861 aatccacaga tttctgatga aaaagacgag gatgaaaagg aggagaagag acgcaaaacg
     1921 acccccaaag aaccaacgga gaaaaaaatg gctcgcgcca aaacagtcat gaactccaag
     1981 acccaccctc ccaagtgcat tcagtgcggg cagtacctgg acgaccctga cctcaaatat
     2041 gggcagcacc caccagacgc ggtggatgag ccacagatgc tgacaaatga gaagctgtcc
     2101 atctttgatg ccaacgagtc tggctttgag agttatgagg cgcttcccca gcacaaactg
     2161 acctgcttca gtgtgtactg taagcacggt cacctgtgtc ccatcgacac cggcctcatc
     2221 gagaagaata tcgaactctt cttttctggt tcagcaaaac caatctatga tgatgaccca
     2281 tctcttgaag gtggtgttaa tggcaaaaat cttggcccca taaatgaatg gtggatcact
     2341 ggctttgatg gaggtgaaaa ggccctcatc ggcttcagca cctcatttgc cgaatacatt
     2401 ctgatggatc ccagtcccga gtatgcgccc atatttgggc tgatgcagga gaagatctac
     2461 atcagcaaga ttgtggtgga gttcctgcag agcaattccg actcgaccta tgaggacctg
     2521 atcaacaaga tcgagaccac ggttcctcct tctggcctca acttgaaccg cttcacagag
     2581 gactccctcc tgcgacacgc gcagtttgtg gtggagcagg tggagagtta tgacgaggcc
     2641 ggggacagtg atgagcagcc catcttcctg acgccctgca tgcgggacct gatcaagctg
     2701 gctggggtca cgctgggaca gaggcgagcc caggcgaggc ggcagaccat caggcattct
     2761 accagggaga aggacagggg acccacgaaa gccaccacca ccaagctggt ctaccagatc
     2821 ttcgatactt tcttcgcaga gcaaattgaa aaggatgaca gagaagacaa ggagaacgcc
     2881 tttaagcgcc ggcgatgtgg cgtctgtgag gtgtgtcagc agcctgagtg tgggaaatgt
     2941 aaagcctgca aggacatggt taaatttggt ggcagtggac ggagcaagca ggcttgccaa
     3001 gagcggaggt gtcccaatat ggccatgaag gaggcagatg acgatgagga agtcgatgat
     3061 aacatcccag agatgccgtc acccaaaaaa atgcaccagg ggaagaagaa gaaacagaac
     3121 aagaatcgca tctcttgggt cggagaagcc gtcaagactg atgggaagaa gagttactat
     3181 aagaaggtgt gcattgatgc ggaaaccctg gaagtggggg actgtgtctc tgttattcca
     3241 gatgattcct caaaaccgct gtatctagca agggtcacgg cgctgtggga ggacagcagc
     3301 aacgggcaga tgtttcacgc ccactggttc tgcgctggga cagacacagt cctcggggcc
     3361 acgtcggacc ctctggagct gttcttggtg gatgaatgtg aggacatgca gctttcatat
     3421 atccacagca aagtgaaagt catctacaaa gccccctccg aaaactgggc catggaggga
     3481 ggcatggatc ccgagtccct gctggagggg gacgacggga agacctactt ctaccagctg
     3541 tggtatgatc aagactacgc gagattcgag tcccctccaa aaacccagcc aacagaggac
     3601 aacaagttca aattctgtgt gagctgtgcc cgtctggctg agatgaggca aaaagaaatc
     3661 cccagggtcc tggagcagct cgaggacctg gatagccggg tcctctacta ctcagccacc
     3721 aagaacggca tcctgtaccg agttggtgat ggtgtgtacc tgccccctga ggccttcacg
     3781 ttcaacatca agctgtccag tcccgtgaaa cgcccacgga aggagcccgt ggatgaggac
     3841 ctgtacccag agcactaccg gaaatactcc gactacatca aaggcagcaa cctggatgcc
     3901 cctgagccct accgaattgg ccggatcaaa gagatcttct gtcccaagaa gagcaacggc
     3961 aggcccaatg agactgacat caaaatccgg gtcaacaagt tctacaggcc tgagaacacc
     4021 cacaagtcca ctccagcgag ctaccacgca gacatcaacc tgctctactg gagcgacgag
     4081 gaggccgtgg tggacttcaa ggctgtgcag ggccgctgca ccgtggagta tggggaggac
     4141 ctgcccgagt gcgtccaggt gtactccatg ggcggcccca accgcttcta cttcctcgag
     4201 gcctataatg caaagagcaa aagctttgaa gatcctccca accatgcccg tagccctgga
     4261 aacaaaggga agggcaaggg aaaagggaag ggcaagccca agtcccaagc ctgtgagccg
     4321 agcgagccag agatagagat caagctgccc aagctgcgga ccctggatgt gttttctggc
     4381 tgcggggggt tgtcggaggg attccaccaa gcaggcatct ctgacacgct gtgggccatc
     4441 gagatgtggg accctgcggc ccaggcgttc cggctgaaca accccggctc cacagtgttc
     4501 acagaggact gcaacatcct gctgaagctg gtcatggctg gggagaccac caactcccgc
     4561 ggccagcggc tgccccagaa gggagacgtg gagatgctgt gcggcgggcc gccctgccag
     4621 ggcttcagcg gcatgaaccg cttcaattcg cgcacctact ccaagttcaa aaactctctg
     4681 gtggtttcct tcctcagcta ctgcgactac taccggcccc ggttcttcct cctggagaat
     4741 gtcaggaact ttgtctcctt caagcgctcc atggtcctga agctcaccct ccgctgcctg
     4801 gtccgcatgg gctatcagtg caccttcggc gtgctgcagg ccggtcagta cggcgtggcc
     4861 cagactagga ggcgggccat catcctggcc gcggcccctg gagagaagct ccctctgttc
     4921 ccggagccac tgcacgtgtt tgctccccgg gcctgccagc tgagcgtggt ggtggatgac
     4981 aagaagtttg tgagcaacat aaccaggttg agctcgggtc ctttccggac catcacggtg
     5041 cgagacacga tgtccgacct gccggaggtg cggaatggag cctcggcact ggagatctcc
     5101 tacaacgggg agcctcagtc ctggttccag aggcagctcc ggggcgcaca gtaccagccc
     5161 atcctcaggg accacatctg taaggacatg agtgcattgg tggctgcccg catgcggcac
     5221 atccccttgg ccccagggtc agactggcgc gatctgccca acatcgaggt gcggctctca
     5281 gacggcacca tggccaggaa gctgcggtat acccaccatg acaggaagaa cggccgcagc
     5341 agctctgggg ccctccgtgg ggtctgctcc tgcgtggaag ccggcaaagc ctgcgacccc
     5401 gcagccaggc agttcaacac cctcatcccc tggtgcctgc cccacaccgg gaaccggcac
     5461 aaccactggg ctggcctcta tggaaggctc gagtgggacg gcttcttcag cacaaccgtc
     5521 accaaccccg agcccatggg caagcagggc cgcgtgctcc acccagagca gcaccgtgtg
     5581 gtgagcgtgc gggagtgtgc ccgctcccag ggcttccctg acacctaccg gctcttcggc
     5641 aacatcctgg acaagcaccg gcaggtgggc aatgccgtgc caccgcccct ggccaaagcc
     5701 attggcttgg agatcaagct ttgtatgttg gccaaagccc gagagagtgc ctcagctaaa
     5761 ataaaggagg aggaagctgc taaggactag gtcgaccgag atctctcgag gtaccgcggc
     5821 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
     5881 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5941 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6001 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6061 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6121 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     6181 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6241 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6301 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6361 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6421 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6481 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6541 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     6601 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6661 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6841 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6961 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     7021 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7081 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7141 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     7201 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     7261 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     7321 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     7381 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     7441 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7501 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     7561 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7621 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7681 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7741 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7801 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     7861 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7921 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8101 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8161 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8221 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8281 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8341 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8401 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8461 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8521 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8581 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8641 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8701 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8761 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8881 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8941 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9001 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9061 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9181 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9241 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9361 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9421 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9481 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9541 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9601 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9661 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9721 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9781 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9841 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9901 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9961 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10021 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10081 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10141 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10201 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10261 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//

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