piRFP

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: piRFP 
出品公司: 爱迪基因
目录编号: 31857
存储实验室: Vladislav Verkhusha 

载体骨架基本信息
载体名称: pEGFP-C1
载体出品公司: Clontech
载体抗性: 卡那霉素
空载体大小: 4700 bp
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 低拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: iRFP
物种来源: 光合细菌 Rhodopseudomonas palustris
插入大小: 948 bp
基因突变: T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2
GenBank编号 JN247409
启动子 CMV
克隆方法: 限制性内切酶
5' 克隆位点: NheI(克隆期间被破坏)
3' 克隆位点: KpnI(克隆期间被破坏)
5' 测序引物 CMV-F
3' 测序引物 SV40pA-R

文献引用方法
材料和方法部分: piRFP was a gift from Vladislav Verkhusha (Addgene plasmid # 31857)
参考文献部分: Bright and stable near-infrared fluorescent protein for in vivo imaging. Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV. Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918. 10.1038/nbt.1918 PubMed 21765402

质粒图谱信息

piRFP载体图谱

质粒简介

质粒序列

LOCUS       piRFP	4937 bp 	DNA	SYN
DEFINITION  piRFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="piRFP"
                     /mol_type="other DNA"
     promoter        10..562
                     /label="CMV_immearly_promoter"
     misc_feature    65..352
                     /label="CAG_enhancer"
     CDS             complement(447..953)
                     /label="ORF frame 1"
     misc_feature    519..539
                     /label="CMV_fwd_primer"
     promoter        520..589
                     /label="CMV_promoter"
     CDS             624..1574
                     /label="ORF frame 3"
     terminator      1618..1846
                     /label="SV40_PA_terminator"
     misc_feature    1816..1835
                     /label="EBV_rev_primer"
     rep_origin      complement(1985..2291)
                     /label="f1_origin"
     promoter        2370..2398
                     /label="AmpR_promoter"
     misc_feature    complement(2464..2484)
                     /label="pBABE_3_primer"
     misc_feature    complement(2470..2685)
                     /label="SV40_enhancer"
     promoter        2482..2750
                     /label="SV40_promoter"
     rep_origin      2649..2726
                     /label="SV40_origin"
     misc_feature    2711..2730
                     /label="SV40pro_F_primer"
     CDS             2833..3627
                     /label="ORF frame 1"
     gene            2836..3624
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(3142..3678)
                     /label="ORF frame 3"
     terminator      3802..4071
                     /label="TK_PA_terminator"
     rep_origin      4219..4838
                     /label="pBR322_origin"
ORIGIN
    1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
   61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
  121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
  181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
  241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
  301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
  361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
  421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
  481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
  541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
  601 ccggactcag atctgccgcc accatggctg aaggatccgt cgccaggcag cctgacctct
  661 tgacctgcga cgatgagccg atccatatcc ccggtgccat ccaaccgcat ggactgctgc
  721 tcgccctcgc cgccgacatg acgatcgttg ccggcagcga caaccttccc gaactcaccg
  781 gactggcgat cggcgccctg atcggccgct ctgcggccga tgtcttcgac tcggagacgc
  841 acaaccgtct gacgatcgcc ttggccgagc ccggggcggc cgtcggagca ccgatcactg
  901 tcggcttcac gatgcgaaag gacgcaggct tcatcggctc ctggcatcgc catgatcagc
  961 tcatcttcct cgagctcgag cctccccagc gggacgtcgc cgagccgcag gcgttcttcc
 1021 gccgcaccaa cagcgccatc cgccgcctgc aggccgccga aaccttggaa agcgcctgcg
 1081 ccgccgcggc gcaagaggtg cggaagatta ccggcttcga tcgggtgatg atctatcgct
 1141 tcgcctccga cttcagcggc gaagtgatcg cagaggatcg gtgcgccgag gtcgagtcaa
 1201 aactaggcct gcactatcct gcctcaaccg tgccggcgca ggcccgtcgg ctctatacca
 1261 tcaacccggt acggatcatt cccgatatca attatcggcc ggtgccggtc accccagacc
 1321 tcaatccggt caccgggcgg ccgattgatc ttagcttcgc catcctgcgc agcgtctcgc
 1381 ccgtccatct ggaattcatg cgcaacatag gcatgcacgg cacgatgtcg atctcgattt
 1441 tgcgcggcga gcgactgtgg ggattgatcg tttgccatca ccgaacgccg tactacgtcg
 1501 atctcgatgg ccgccaagcc tgcgagctag tcgcccaggt tctggcctgg cagatcggcg
 1561 tgatggaaga gtgagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
 1621 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
 1681 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
 1741 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
 1801 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
 1861 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
 1921 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
 1981 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
 2041 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
 2101 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
 2161 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
 2221 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
 2281 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
 2341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
 2401 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
 2461 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
 2521 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
 2581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
 2641 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
 2701 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
 2761 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
 2821 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
 2881 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
 2941 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
 3001 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
 3061 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
 3121 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
 3181 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
 3241 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
 3301 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
 3361 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
 3421 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
 3481 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
 3541 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
 3601 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
 3661 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
 3721 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
 3781 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
 3841 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
 3901 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
 3961 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
 4021 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
 4081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
 4141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
 4201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
 4261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
 4321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
 4381 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
 4441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
 4501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
 4561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
 4621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
 4681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
 4741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
 4801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
 4861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
 4921 gtattaccgc catgcat
//

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