| 质粒名称: | pAAV-fSST-RFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22913 |
| 存储实验室: | Edward Callaway |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4000 bp |
| 完整质粒大小: | 8300 bp |
| 载体类型: | AAV,哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | fugu somatostatin promoter driving DsRed 2 |
| 物种来源: | takifugu rubripes (河豚) |
| 插入大小: | 4300 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | Unknown |
| 3' 测序引物 | SP6 |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-fSST-RFP was a gift from Edward Callaway (爱迪基因 plasmid # 22913) |
| 参考文献部分: | Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM. Front Neural Circuits. 2009 . 3():19. 10.3389/neuro.04.019.2009 PubMed 19949461 |
pAAV-fSST-RFP
价格：1000元
联系方式：I47-825O-882O
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质粒基本信息
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LOCUS pAAV-fSST-RFP 8109 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-fSST-RFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8109)
AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
Callaway EM
TITLE Short Promoters in Viral Vectors Drive Selective Expression in
Mammalian Inhibitory Neurons, but do not Restrict Activity to
Specific Inhibitory Cell-Types.
JOURNAL Front Neural Circuits. 2009 . 3():19.
PUBMED 19949461
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-fSST-RFP|
FEATURES Location/Qualifiers
source 1..8109
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 98..115
/label=L4440
/note="L4440 vector, forward primer"
CDS 2671..2700
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
CDS 2674..2703
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
intron 2898..3373
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
misc_feature 3476..3528
/label=MCS
/note="multiple cloning site"
regulatory 3536..3545
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3542..4219
/codon_start=1
/product="improved tetrameric variant of DsRed fluorescent
protein"
/label=DsRed2
/note="mammalian codon-optimized"
/translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
LFL"
primer_bind complement(3722..3742)
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind 4134..4157
/label=DsRed1-C
/note="DsRed1, forward primer"
promoter complement(4270..4288)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4271..4288)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4308..4325)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4314..4425
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4569..4698
/label=AAV2 ITR
rep_origin 5069..5582
/direction=RIGHT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5156..5175)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5366..5387
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6064..6083)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6183..6205
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6243..6261)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6329..6433
/gene="bla"
/label=AmpR promoter
CDS 6434..7294
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6652..6671)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7465..8053
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7954..7973
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgagcgagc
121 gcgcagagag ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc
181 gccatgctac ttatctacgt agccatgctc tagaggatcc ttcgaaaagc ttgtatacgg
241 ccggccacgc gtagctgatg ctcagcagga atccagatga ttccctctga gcgaagacaa
301 aatgagagaa agtaaaatgg agagaaggaa aaagaagatg gtgacaggga attggagaaa
361 atcaaggaca agggaataac aaaaggaaac acaattaaag aagagaatag atggaaagac
421 aaggacacaa atcaagtgca gggacataaa aaaatgttct gtgaatgagt gtacaagata
481 tgatgtaaat catatgcagt gtcatgtttc aagaaatgga aaaggaaaaa aaacagtgag
541 acaaacaggc acattggcag acagacaagt ggcagatggg catgcaattt cagcattttt
601 tgaatttttt acatttgtaa tttaataata ataatttaat catttaagac aattgttaaa
661 actcttcctc cactcagcat ccatgtgaga cacttcttgg gagtcggatt gggtgctgca
721 tcatatgata acggtcggac aagtgtgaaa aacagtacat tctttcattg gattatagtc
781 atttgactgc tgaagtccag ccatctcatc actggaagag ccaggtcagt gcagctagag
841 atgaatgaat aattgtatct cttagaaaac gagttaataa tgatgtttcc tgttttattt
901 tccatttgct tcctgtgttt tttattgatg tcagatgccc tcatgggctc cacccttctt
961 tacatgccag gtgttattcc cctttatttt tttgtgtgct gtctgggact gcaagaagcc
1021 attccttagg ctaatgtttg ccacacagaa acaacttgtc tgattgttga atttatgaat
1081 gatacaagtt ctccaattat ttttgtgaaa tttttatttt tatacatttc tctgtgtggt
1141 actctgggca ttgttctctt cactcctata cagtagaaac accacccaat caaatgccat
1201 cattataggt tttgtttgac gctgaacaca acgcagaaca taagttctga agatattttt
1261 gaaaaccaaa gatgtttgtg gctgtgttcc tttctcacag cggagtttga tcagtgttct
1321 tggtagaaca gtggggaagt gtacaaacac tttaactgct gtgattattc atggatctcc
1381 cacagattcc acatattact gaacattaac actcaacagg acatggctac cagtttagag
1441 aaaatattcc ctgtggaaca caaacgctga gtgaacttct ctcacagtta acctccaatc
1501 gagtcgacaa gaaacctgaa gaatatctga gcttgaattt gtctgtgatt ggttcagatc
1561 aacactggtc aactgatgtc tgctctcttg aagcaatttt cattgtttta aatgcaaatc
1621 taaaaactaa ccctgaagat gggtgggaaa tacagcaagc aataccctaa cccagattga
1681 tgattatatc agaaatgaac taggacccag ggtccaggtt caggactaca aacatcatgt
1741 gggttagggt cagaggttcg aaaaagacaa tggattgtta gccatttaga cacatcaact
1801 ccacatctgt gtgtacgagt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
1861 gtgtgtgtga gagagagaga gagagcgaga gggagtgaga gagagagcga gtatgtgtgt
1921 gtcttcatat ttactacata ttaagtactc ccaaaggatt gttggagggt taggacacgg
1981 ttgaggttgg aattgaattc aggtcagggt tagtgtcagc gtcagtgtca gggtcaggat
2041 cagtgtttga gttatgttta agctgctggt tagaacagaa aggaataagg gtaaggagct
2101 gagtaatgta ttatatgtcc tcaaaaagac agaaatgtga aagtgtgtgt gtgtgtgtgt
2161 gtgcgtgtgt gtgtttttgt gtgtgtgcac gtgccaacac ctttgtcttg ccttggttac
2221 agttagtgac atggagtgtg tggttacgtg cttaagggct ggtcttgagt agttcttgtg
2281 cttcttctgt ctcaggcatc atcaacctga aagttttcat tgttttctga gattttgttg
2341 ttgtttacag ctttatagta gcagtgtttc attcccacaa tgcattgttt tgcagctcaa
2401 ctatgaatca attattagtc ttttaaatta tattatgtac tcgtgttgta cttaaaagtt
2461 aactgcaatg tttcaatgtt tggttcaact ctcattttat ttaataaata atgcagaaag
2521 ttactgataa ctaatgtcat tatcaactat tgatcattcc tggatcctgt gagcacagtt
2581 tgaacacatg caagtgtgcg tcatcttgca gaggaacttt atgaagcagt gggaagtaaa
2641 gagaacgggt cattagactg aagcagcaaa catcatcatc atcatcatca tcatcatcat
2701 catctctttc ttttttcccc tctctaacag actcactaac attcacatgc acacacagca
2761 tgacgtcagc ggaatggggg ctatatatga gatgagctca gatggaaaag agccacaaga
2821 tctgcagagc cagtgacaga aacacttagt tgggtaccgc tagcggcgcg ccgagctcgg
2881 atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc ccttcttttc
2941 tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata ttgaccaaat
3001 cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg
3061 tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg
3121 tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat
3181 agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc
3241 atattgctaa tagcagctac aatccagcta ccattctgct tttattttat ggttgggata
3301 aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat
3361 cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa
3421 agcacgtgaa ttcgaagatc tggccgcctg ggccggtgac cgtaccgggc cccccctcga
3481 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
3541 catggcctcc tccgagaacg tcatcaccga gttcatgcgc ttcaaggtgc gcatggaggg
3601 caccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
3661 ccacaacacc gtgaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
3721 cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc
3781 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
3841 ggacggcggc gtggcgaccg tgacccagga ctcctccctg caggacggct gcttcatcta
3901 caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtgatgc agaagaagac
3961 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
4021 gacccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat
4081 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggacg ccaagctgga
4141 catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgca ccgagggccg
4201 ccaccacctg ttcctgtagc ggccgccacc gcggtggagc tgtttaaacc gtacgggaaa
4261 cggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
4321 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
4381 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
4441 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
4501 caatagcgcg gccgctctag agcatggcta cgtagataag tagcatggcg ggttaatcat
4561 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
4621 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
4681 gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
4741 acagttgcgc agcctgaatg gcgaatggcg attccgttgc aatggctggc ggtaatattg
4801 ttctggatat taccagcaag gccgatagtt tgagttcttc tactcaggca agtgatgtta
4861 ttactaatca aagaagtatt gcgacaacgg ttaatttgcg tgatggacag actcttttac
4921 tcggtggcct cactgattat aaaaacactt ctcaggattc tggcgtaccg ttcctgtcta
4981 aaatcccttt aatcggcctc ctgtttagct cccgctctga ttctaacgag gaaagcacgt
5041 tatacgtgct cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
5101 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
5161 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
5221 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
5281 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
5341 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
5401 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
5461 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
5521 atttaaatat ttgcttatac aatcttcctg tttttggggc ttttctgatt atcaaccggg
5581 gtacatatga ttgacatgct agttttacga ttaccgttca tcgattctct tgtttgctcc
5641 agactctcag gcaatgacct gatagccttt gtagagacct ctcaaaaata gctaccctct
5701 ccggcatgaa tttatcagct agaacggttg aatatcatat tgatggtgat ttgactgtct
5761 ccggcctttc tcacccgttt gaatctttac ctacacatta ctcaggcatt gcatttaaaa
5821 tatatgaggg ttctaaaaat ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag
5881 tattacaggg tcataatgtt tttggtacaa ccgatttagc tttatgctct gaggctttat
5941 tgcttaattt tgctaattct ttgccttgcc tgtatgattt attggatgtt ggaatcgcct
6001 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
6061 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
6121 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
6181 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
6241 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
6301 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
6361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
6421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
6481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
6541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
6601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
6661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
6721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
6781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
6841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
6901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
6961 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
7021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
7081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
7141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
7201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
7261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
7321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
7381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
7441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
7501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
7561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
7621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
7681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
7741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
7801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
7861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
7921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
7981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
8041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
8101 ttttgctca
//
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