pBiFC-bFosVC155

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: pBiFC-bFosVC155 
出品公司: 爱迪基因
目录编号: 22013
存储实验室: Chang-Deng Hu

载体骨架基本信息
载体名称: pCMV-HA
载体出品公司: Clontech
载体抗性: 氨苄霉素
空载体大小: 3759 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: bFosVC155
物种来源: R. norvegicus (rat); Aequorea Victoria
插入大小: 593 bp
基因突变: N/A
标签/融合蛋白: HA(N端)
克隆方法: 限制性内切酶
5' 克隆位点: EcoRI(未破坏)
3' 克隆位点: NotI (未破坏)
5' 测序引物 CMV-F
3' 测序引物 EBV-Rev

文献引用方法
材料和方法部分: pBiFC-bFosVC155 was a gift from Chang-Deng Hu (Addgene plasmid # 22013)
参考文献部分: Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Jan . 40(1):61-6. 10.2144/000112036 PubMed 16454041

质粒图谱信息

pBiFC-bFosVC155载体图谱

质粒简介

质粒序列

LOCUS       pBiFC-bFosVC155	4352 bp 	DNA	SYN
DEFINITION  pBiFC-bFosVC155
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="pBiFC-bFosVC155"
                     /mol_type="other DNA"
     misc_feature    31..318
                     /label="CAG_enhancer"
     promoter        36..528
                     /label="CMV_immearly_promoter"
     misc_feature    485..505
                     /label="CMV_fwd_primer"
     promoter        486..555
                     /label="CMV_promoter"
     promoter        498..617
                     /label="CMV2_promoter"
     misc_feature    529..548
                     /label="pCEP_fwd_primer"
     misc_feature    531..555
                     /label="LNCX_primer"
     misc_feature    631..657
                     /label="pCMV_primer"
     misc_feature    672..768
                     /label="modSV40_late_16s_int"
     misc_feature    672..702
                     /label="SV40_late_19s_int"
     CDS             829..1479
                     /label="ORF frame 1"
     misc_feature    832..858
                     /label="HA"
     gene            1231..1476
                     /label="ECFP (468 - 713)"
                     /gene="ECFP (468 - 713)"
     misc_feature    1413..1434
                     /label="EGFP_C_primer"
     terminator      complement(1501..1620)
                     /label="SV40_PA_terminator"
     misc_feature    complement(1515..1534)
                     /label="EBV_rev_primer"
     promoter        complement(1732..1750)
                     /label="M13_reverse_primer"
     misc_feature    complement(1749..1771)
                     /label="M13_pUC_rev_primer"
     promoter        complement(1785..1814)
                     /label="lac_promoter"
     gene            complement(2897..3757)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2897..3757)
                     /label="ORF frame 2"
     promoter        complement(3799..3827)
                     /label="AmpR_promoter"
     misc_feature    complement(3986..4008)
                     /label="pGEX_3_primer"
     misc_feature    complement(4207..4350)
                     /label="lacZ_a"
     misc_feature    4321..4343
                     /label="M13_pUC_fwd_primer"
     promoter        4336..4352
                     /label="M13_forward20_primer"
ORIGIN
    1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
   61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
  121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
  181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
  241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
  301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
  361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
  421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
  481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
  541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
  601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG
  661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
  721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
  781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC
  841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTCGGGGTCG TGCGCAGTCC
  901 ATCGGTCGTC GCGGTAAAGT TGAACAACTG TCCCCGGAAG AGGAAGAGAA ACGTCGCATC
  961 CGCCGTGAAC GTAACAAAAT GGCGGCAGCG AAATGCCGTA ACCGCCGTCG TGAACTGACC
 1021 GACACCCTGC AGGCGGAAAC CGACCAGCTG GAAGACGAAA AATCCGCGCT GCAAACCGAA
 1081 ATCGCGAACC TGCTGAAAGA AAAAGAAAAG CTGGAGTTCA TCCTGGCGGC ACACCGTCCG
 1141 GCGTGCAAAA TCCCGAACGA CCTGGGTGGT ACCCGTCCGG CGTGCAAAAT CCCGAACGAC
 1201 CTGAAACAGA AAGTCATGAA CCACGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG
 1261 ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC
 1321 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCAAA
 1381 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC
 1441 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG CGGCCGCGGG GATCCAGACA
 1501 TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT
 1561 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC
 1621 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG
 1681 TTTTTTCGGA TCCTCTAGAG TCGATCTGCA GGCATGCTAG CTTGGCGTAA TCATGGTCAT
 1741 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
 1801 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC
 1861 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC
 1921 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT
 1981 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
 2041 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
 2101 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
 2161 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
 2221 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
 2281 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
 2341 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
 2401 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
 2461 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
 2521 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
 2581 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
 2641 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
 2701 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
 2761 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
 2821 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
 2881 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
 2941 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
 3001 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
 3061 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
 3121 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
 3181 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
 3241 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
 3301 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
 3361 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
 3421 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
 3481 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
 3541 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
 3601 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
 3661 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
 3721 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
 3781 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
 3841 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA
 3901 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG
 3961 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG
 4021 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG
 4081 GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC
 4141 ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT
 4201 CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC
 4261 GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT
 4321 CCCAGTCACG ACGTTGTAAA ACGACGGCCA GT
//

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