pY004 (pcDNA3.1-hFnCpf1)

LOCUS       pY004 (pcDNA3.1-hFnCpf1)	9443 bp 	DNA	SYN
DEFINITION  pY004 (pcDNA3.1-hFnCpf1)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="pY004 (pcDNA3.1-hFnCpf1)"
                     /mol_type="other DNA"
     promoter        236..852
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     misc_feature    769..789
                     /label="CMV_fwd_primer"
     promoter        863..881
                     /label="T7_promoter"
     CDS             929..4966
                     /label="ORF frame 2"
     misc_feature    4883..4909
                     /label="HA"
     misc_feature    complement(5037..5054)
                     /label="BGH_rev_primer"
     terminator      5040..5267
                     /label="bGH_PA_terminator"
     rep_origin      5330..5636
                     /label="f1_origin"
     misc_feature    complement(5750..5770)
                     /label="pBABE_3_primer"
     misc_feature    complement(5756..5971)
                     /label="SV40_enhancer"
     promoter        5768..6036
                     /label="SV40_promoter"
     rep_origin      5935..6012
                     /label="SV40_origin"
     misc_feature    5997..6016
                     /label="SV40pro_F_primer"
     CDS             6151..6945
                     /label="ORF frame 1"
     gene            6154..6942
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(6460..6996)
                     /label="ORF frame 3"
     terminator      7122..7241
                     /label="SV40_PA_terminator"
     misc_feature    7210..7229
                     /label="EBV_rev_primer"
     promoter        complement(7285..7303)
                     /label="M13_reverse_primer"
     misc_feature    complement(7302..7324)
                     /label="M13_pUC_rev_primer"
     promoter        complement(7338..7367)
                     /label="lac_promoter"
     gene            complement(8447..9307)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8447..9307)
                     /label="ORF frame 2"
     promoter        complement(9349..9377)
                     /label="AmpR_promoter"
ORIGIN
    1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
   61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
  121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
  181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
  241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
  301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
  361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
  421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
  481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
  541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
  601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
  661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
  721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
  781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
  841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
  901 gtttaaactt aagcttggta ccgccaccat gagcatctac caggagttcg tcaacaagta
  961 ttcactgagt aagacactgc ggttcgagct gatcccacag ggcaagacac tggagaacat
 1021 caaggcccga ggcctgattc tggacgatga gaagcgggca aaagactata agaaagccaa
 1081 gcagatcatt gataaatacc accagttctt tatcgaggaa attctgagct ccgtgtgcat
 1141 cagtgaggat ctgctgcaga attactcaga cgtgtacttc aagctgaaga agagcgacga
 1201 tgacaacctg cagaaggact tcaagtccgc caaggacacc atcaagaaac agattagcga
 1261 gtacatcaag gactccgaaa agtttaaaaa tctgttcaac cagaatctga tcgatgctaa
 1321 gaaaggccag gagtccgacc tgatcctgtg gctgaaacag tctaaggaca atgggattga
 1381 actgttcaag gctaactccg atatcactga tattgacgag gcactggaaa tcatcaagag
 1441 cttcaaggga tggaccacat actttaaagg cttccacgag aaccgcaaga acgtgtactc
 1501 cagcaacgac attcctacct ccatcatcta ccgaatcgtc gatgacaatc tgccaaagtt
 1561 cctggagaac aaggccaaat atgaatctct gaaggacaaa gctcccgagg caattaatta
 1621 cgaacagatc aagaaagatc tggctgagga actgacattc gatatcgact ataagactag
 1681 cgaggtgaac cagagggtct tttccctgga cgaggtgttt gaaatcgcca atttcaacaa
 1741 ttacctgaac cagtccggca ttactaaatt caataccatc attggcggga agtttgtgaa
 1801 cggggagaat accaagcgca agggaattaa cgaatacatc aatctgtata gccagcagat
 1861 caacgacaaa actctgaaga aatacaagat gtctgtgctg ttcaaacaga tcctgagtga
 1921 taccgagtcc aagtcttttg tcattgataa actggaagat gactcagacg tggtcactac
 1981 catgcagagc ttttatgagc agatcgccgc tttcaagaca gtggaggaaa aatctattaa
 2041 ggaaactctg agtctgctgt tcgatgacct gaaagcccag aagctggacc tgagtaagat
 2101 ctacttcaaa aacgataaga gtctgacaga cctgtcacag caggtgtttg atgactattc
 2161 cgtgattggg accgccgtcc tggagtacat tacacagcag atcgctccaa agaacctgga
 2221 taatccctct aagaaagagc aggaactgat cgctaagaaa accgagaagg caaaatatct
 2281 gagtctggaa acaattaagc tggcactgga ggagttcaac aagcacaggg atattgacaa
 2341 acagtgccgc tttgaggaaa tcctggccaa cttcgcagcc atccccatga tttttgatga
 2401 gatcgcccag aacaaagaca atctggctca gatcagtatt aagtaccaga accagggcaa
 2461 gaaagacctg ctgcaggctt cagcagaaga tgacgtgaaa gccatcaagg atctgctgga
 2521 ccagaccaac aatctgctgc acaagctgaa aatcttccat attagtcagt cagaggataa
 2581 ggctaatatc ctggataaag acgaacactt ctacctggtg ttcgaggaat gttacttcga
 2641 gctggcaaac attgtccccc tgtataacaa gattaggaac tacatcacac agaagcctta
 2701 ctctgacgag aagtttaaac tgaacttcga aaatagtacc ctggccaacg ggtgggataa
 2761 gaacaaggag cctgacaaca cagctatcct gttcatcaag gatgacaagt actatctggg
 2821 agtgatgaat aagaaaaaca ataagatctt cgatgacaaa gccattaagg agaacaaagg
 2881 ggaaggatac aagaaaatcg tgtataagct gctgcccggc gcaaataaga tgctgcctaa
 2941 ggtgttcttc agcgccaaga gtatcaaatt ctacaaccca tccgaggaca tcctgcggat
 3001 tagaaatcac tcaacacata ctaagaacgg gagcccccag aagggatatg agaaatttga
 3061 gttcaacatc gaggattgca ggaagtttat tgacttctac aagcagagca tctccaaaca
 3121 ccctgaatgg aaggattttg gcttccggtt ttccgacaca cagagatata actctatcga
 3181 cgagttctac cgcgaggtgg aaaatcaggg gtataagctg acttttgaga acatttctga
 3241 aagttacatc gacagcgtgg tcaatcaggg aaagctgtac ctgttccaga tctataacaa
 3301 agatttttca gcatacagca agggcagacc aaacctgcat acactgtact ggaaggccct
 3361 gttcgatgag aggaatctgc aggacgtggt ctataaactg aacggagagg ccgaactgtt
 3421 ttaccggaag cagtctattc ctaagaaaat cactcaccca gctaaggagg ccatcgctaa
 3481 caagaacaag gacaatccta agaaagagag cgtgttcgaa tacgatctga ttaaggacaa
 3541 gcggttcacc gaagataagt tctttttcca ttgtccaatc accattaact tcaagtcaag
 3601 cggcgctaac aagttcaacg acgagatcaa tctgctgctg aaggaaaaag caaacgatgt
 3661 gcacatcctg agcattgacc gaggagagcg gcatctggcc tactataccc tggtggatgg
 3721 caaagggaat atcattaagc aggatacatt caacatcatt ggcaatgacc ggatgaaaac
 3781 caactaccac gataaactgg ctgcaatcga gaaggataga gactcagcta ggaaggactg
 3841 gaagaaaatc aacaacatta aggagatgaa ggaaggctat ctgagccagg tggtccatga
 3901 gattgcaaag ctggtcatcg aatacaatgc cattgtggtg ttcgaggatc tgaacttcgg
 3961 ctttaagagg gggcgcttta aggtggaaaa acaggtctat cagaagctgg agaaaatgct
 4021 gatcgaaaag ctgaattacc tggtgtttaa agataacgag ttcgacaaga ccggaggcgt
 4081 cctgagagcc taccagctga cagctccctt tgaaactttc aagaaaatgg gaaaacagac
 4141 aggcatcatc tactatgtgc cagccggatt cacttccaag atctgccccg tgaccggctt
 4201 tgtcaaccag ctgtacccta aatatgagtc agtgagcaag tcccaggaat ttttcagcaa
 4261 gttcgataag atctgttata atctggacaa ggggtacttc gagttttcct tcgattacaa
 4321 gaacttcggc gacaaggccg ctaaggggaa atggaccatt gcctccttcg gatctcgcct
 4381 gatcaacttt cgaaattccg ataaaaacca caattgggac actagggagg tgtacccaac
 4441 caaggagctg gaaaagctgc tgaaagacta ctctatcgag tatggacatg gcgaatgcat
 4501 caaggcagcc atctgtggcg agagtgataa gaaatttttc gccaagctga cctcagtgct
 4561 gaatacaatc ctgcagatgc ggaactcaaa gaccgggaca gaactggact atctgattag
 4621 ccccgtggct gatgtcaacg gaaacttctt cgacagcaga caggcaccca aaaatatgcc
 4681 tcaggatgca gacgccaacg gggcctacca catcgggctg aagggactga tgctgctggg
 4741 ccggatcaag aacaatcagg aggggaagaa gctgaacctg gtcattaaga acgaggaata
 4801 cttcgagttt gtccagaata gaaataacaa aaggccggcg gccacgaaaa aggccggcca
 4861 ggcaaaaaag aaaaagggat cctacccata cgatgttcca gattacgctt atccctacga
 4921 cgtgcctgat tatgcatacc catatgatgt ccccgactat gcctaagaat tctgcagata
 4981 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
 5041 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
 5101 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
 5161 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
 5221 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
 5281 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
 5341 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
 5401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
 5461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
 5521 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
 5581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
 5641 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
 5701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
 5761 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
 5821 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
 5881 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
 5941 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
 6001 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
 6061 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
 6121 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
 6181 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
 6241 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
 6301 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
 6361 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
 6421 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
 6481 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
 6541 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
 6601 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
 6661 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
 6721 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
 6781 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
 6841 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
 6901 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
 6961 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
 7021 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
 7081 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
 7141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
 7201 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
 7261 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
 7321 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
 7381 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
 7441 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
 7501 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
 7561 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
 7621 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
 7681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
 7741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
 7801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
 7861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
 7921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
 7981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
 8041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
 8101 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
 8161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
 8221 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
 8281 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
 8341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
 8401 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
 8461 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
 8521 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
 8581 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
 8641 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
 8701 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
 8761 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
 8821 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
 8881 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
 8941 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
 9001 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
 9061 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
 9121 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
 9181 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
 9241 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
 9301 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
 9361 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
 9421 cccgaaaagt gccacctgac gtc
//