pY010 (pcDNA3.1-hAsCpf1)

LOCUS       pY010 (pcDNA3.1-hAsCpf1)	9464 bp 	DNA	SYN
DEFINITION  pY010 (pcDNA3.1-hAsCpf1)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="pY010 (pcDNA3.1-hAsCpf1)"
                     /mol_type="other DNA"
     promoter        236..852
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     misc_feature    769..789
                     /label="CMV_fwd_primer"
     promoter        863..881
                     /label="T7_promoter"
     CDS             929..4987
                     /label="ORF frame 2"
     CDS             complement(1663..2199)
                     /label="ORF frame 3"
     misc_feature    4904..4930
                     /label="HA"
     misc_feature    complement(5058..5075)
                     /label="BGH_rev_primer"
     terminator      5061..5288
                     /label="bGH_PA_terminator"
     rep_origin      5351..5657
                     /label="f1_origin"
     misc_feature    complement(5771..5791)
                     /label="pBABE_3_primer"
     misc_feature    complement(5777..5992)
                     /label="SV40_enhancer"
     promoter        5789..6057
                     /label="SV40_promoter"
     rep_origin      5956..6033
                     /label="SV40_origin"
     misc_feature    6018..6037
                     /label="SV40pro_F_primer"
     CDS             6172..6966
                     /label="ORF frame 1"
     gene            6175..6963
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(6481..7017)
                     /label="ORF frame 3"
     terminator      7143..7262
                     /label="SV40_PA_terminator"
     misc_feature    7231..7250
                     /label="EBV_rev_primer"
     promoter        complement(7306..7324)
                     /label="M13_reverse_primer"
     misc_feature    complement(7323..7345)
                     /label="M13_pUC_rev_primer"
     promoter        complement(7359..7388)
                     /label="lac_promoter"
     gene            complement(8468..9328)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8468..9328)
                     /label="ORF frame 2"
     promoter        complement(9370..9398)
                     /label="AmpR_promoter"
ORIGIN
    1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
   61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
  121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
  181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
  241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
  301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
  361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
  421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
  481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
  541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
  601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
  661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
  721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
  781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
  841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
  901 gtttaaactt aagcttggta ccgccaccAT GACACAGTTC GAGGGCTTTA CCAACCTGTA
  961 TCAGGTGAGC AAGACACTGC GGTTTGAGCT GATCCCACAG GGCAAGACCC TGAAGCACAT
 1021 CCAGGAGCAG GGCTTCATCG AGGAGGACAA GGCCCGCAAT GATCACTACA AGGAGCTGAA
 1081 GCCCATCATC GATCGGATCT ACAAGACCTA TGCCGACCAG TGCCTGCAGC TGGTGCAGCT
 1141 GGATTGGGAG AACCTGAGCG CCGCCATCGA CTCCTATAGA AAGGAGAAAA CCGAGGAGAC
 1201 AAGGAACGCC CTGATCGAGG AGCAGGCCAC ATATCGCAAT GCCATCCACG ACTACTTCAT
 1261 CGGCCGGACA GACAACCTGA CCGATGCCAT CAATAAGAGA CACGCCGAGA TCTACAAGGG
 1321 CCTGTTCAAG GCCGAGCTGT TTAATGGCAA GGTGCTGAAG CAGCTGGGCA CCGTGACCAC
 1381 AACCGAGCAC GAGAACGCCC TGCTGCGGAG CTTCGACAAG TTTACAACCT ACTTCTCCGG
 1441 CTTTTATGAG AACAGGAAGA ACGTGTTCAG CGCCGAGGAT ATCAGCACAG CCATCCCACA
 1501 CCGCATCGTG CAGGACAACT TCCCCAAGTT TAAGGAGAAT TGTCACATCT TCACACGCCT
 1561 GATCACCGCC GTGCCCAGCC TGCGGGAGCA CTTTGAGAAC GTGAAGAAGG CCATCGGCAT
 1621 CTTCGTGAGC ACCTCCATCG AGGAGGTGTT TTCCTTCCCT TTTTATAACC AGCTGCTGAC
 1681 ACAGACCCAG ATCGACCTGT ATAACCAGCT GCTGGGAGGA ATCTCTCGGG AGGCAGGCAC
 1741 CGAGAAGATC AAGGGCCTGA ACGAGGTGCT GAATCTGGCC ATCCAGAAGA ATGATGAGAC
 1801 AGCCCACATC ATCGCCTCCC TGCCACACAG ATTCATCCCC CTGTTTAAGC AGATCCTGTC
 1861 CGATAGGAAC ACCCTGTCTT TCATCCTGGA GGAGTTTAAG AGCGACGAGG AAGTGATCCA
 1921 GTCCTTCTGC AAGTACAAGA CACTGCTGAG AAACGAGAAC GTGCTGGAGA CAGCCGAGGC
 1981 CCTGTTTAAC GAGCTGAACA GCATCGACCT GACACACATC TTCATCAGCC ACAAGAAGCT
 2041 GGAGACAATC AGCAGCGCCC TGTGCGACCA CTGGGATACA CTGAGGAATG CCCTGTATGA
 2101 GCGGAGAATC TCCGAGCTGA CAGGCAAGAT CACCAAGTCT GCCAAGGAGA AGGTGCAGCG
 2161 CAGCCTGAAG CACGAGGATA TCAACCTGCA GGAGATCATC TCTGCCGCAG GCAAGGAGCT
 2221 GAGCGAGGCC TTCAAGCAGA AAACCAGCGA GATCCTGTCC CACGCACACG CCGCCCTGGA
 2281 TCAGCCACTG CCTACAACCC TGAAGAAGCA GGAGGAGAAG GAGATCCTGA AGTCTCAGCT
 2341 GGACAGCCTG CTGGGCCTGT ACCACCTGCT GGACTGGTTT GCCGTGGATG AGTCCAACGA
 2401 GGTGGACCCC GAGTTCTCTG CCCGGCTGAC CGGCATCAAG CTGGAGATGG AGCCTTCTCT
 2461 GAGCTTCTAC AACAAGGCCA GAAATTATGC CACCAAGAAG CCCTACTCCG TGGAGAAGTT
 2521 CAAGCTGAAC TTTCAGATGC CTACACTGGC CTCTGGCTGG GACGTGAATA AGGAGAAGAA
 2581 CAATGGCGCC ATCCTGTTTG TGAAGAACGG CCTGTACTAT CTGGGCATCA TGCCAAAGCA
 2641 GAAGGGCAGG TATAAGGCCC TGAGCTTCGA GCCCACAGAG AAAACCAGCG AGGGCTTTGA
 2701 TAAGATGTAC TATGACTACT TCCCTGATGC CGCCAAGATG ATCCCAAAGT GCAGCACCCA
 2761 GCTGAAGGCC GTGACAGCCC ACTTTCAGAC CCACACAACC CCCATCCTGC TGTCCAACAA
 2821 TTTCATCGAG CCTCTGGAGA TCACAAAGGA GATCTACGAC CTGAACAATC CTGAGAAGGA
 2881 GCCAAAGAAG TTTCAGACAG CCTACGCCAA GAAAACCGGC GACCAGAAGG GCTACAGAGA
 2941 GGCCCTGTGC AAGTGGATCG ACTTCACAAG GGATTTTCTG TCCAAGTATA CCAAGACAAC
 3001 CTCTATCGAT CTGTCTAGCC TGCGGCCATC CTCTCAGTAT AAGGACCTGG GCGAGTACTA
 3061 TGCCGAGCTG AATCCCCTGC TGTACCACAT CAGCTTCCAG AGAATCGCCG AGAAGGAGAT
 3121 CATGGATGCC GTGGAGACAG GCAAGCTGTA CCTGTTCCAG ATCTATAACA AGGACTTTGC
 3181 CAAGGGCCAC CACGGCAAGC CTAATCTGCA CACACTGTAT TGGACCGGCC TGTTTTCTCC
 3241 AGAGAACCTG GCCAAGACAA GCATCAAGCT GAATGGCCAG GCCGAGCTGT TCTACCGCCC
 3301 TAAGTCCAGG ATGAAGAGGA TGGCACACCG GCTGGGAGAG AAGATGCTGA ACAAGAAGCT
 3361 GAAGGATCAG AAAACCCCAA TCCCCGACAC CCTGTACCAG GAGCTGTACG ACTATGTGAA
 3421 TCACAGACTG TCCCACGACC TGTCTGATGA GGCCAGGGCC CTGCTGCCCA ACGTGATCAC
 3481 CAAGGAGGTG TCTCACGAGA TCATCAAGGA TAGGCGCTTT ACCAGCGACA AGTTCTTTTT
 3541 CCACGTGCCT ATCACACTGA ACTATCAGGC CGCCAATTCC CCATCTAAGT TCAACCAGAG
 3601 GGTGAATGCC TACCTGAAGG AGCACCCCGA GACACCTATC ATCGGCATCG ATCGGGGCGA
 3661 GAGAAACCTG ATCTATATCA CAGTGATCGA CTCCACCGGC AAGATCCTGG AGCAGCGGAG
 3721 CCTGAACACC ATCCAGCAGT TTGATTACCA GAAGAAGCTG GACAACAGGG AGAAGGAGAG
 3781 GGTGGCAGCA AGGCAGGCCT GGTCTGTGGT GGGCACAATC AAGGATCTGA AGCAGGGCTA
 3841 TCTGAGCCAG GTCATCCACG AGATCGTGGA CCTGATGATC CACTACCAGG CCGTGGTGGT
 3901 GCTGGAGAAC CTGAATTTCG GCTTTAAGAG CAAGAGGACC GGCATCGCCG AGAAGGCCGT
 3961 GTACCAGCAG TTCGAGAAGA TGCTGATCGA TAAGCTGAAT TGCCTGGTGC TGAAGGACTA
 4021 TCCAGCAGAG AAAGTGGGAG GCGTGCTGAA CCCATACCAG CTGACAGACC AGTTCACCTC
 4081 CTTTGCCAAG ATGGGCACCC AGTCTGGCTT CCTGTTTTAC GTGCCTGCCC CATATACATC
 4141 TAAGATCGAT CCCCTGACCG GCTTCGTGGA CCCCTTCGTG TGGAAAACCA TCAAGAATCA
 4201 CGAGAGCCGC AAGCACTTCC TGGAGGGCTT CGACTTTCTG CACTACGACG TGAAAACCGG
 4261 CGACTTCATC CTGCACTTTA AGATGAACAG AAATCTGTCC TTCCAGAGGG GCCTGCCCGG
 4321 CTTTATGCCT GCATGGGATA TCGTGTTCGA GAAGAACGAG ACACAGTTTG ACGCCAAGGG
 4381 CACCCCTTTC ATCGCCGGCA AGAGAATCGT GCCAGTGATC GAGAATCACA GATTCACCGG
 4441 CAGATACCGG GACCTGTATC CTGCCAACGA GCTGATCGCC CTGCTGGAGG AGAAGGGCAT
 4501 CGTGTTCAGG GATGGCTCCA ACATCCTGCC AAAGCTGCTG GAGAATGACG ATTCTCACGC
 4561 CATCGACACC ATGGTGGCCC TGATCCGCAG CGTGCTGCAG ATGCGGAACT CCAATGCCGC
 4621 CACAGGCGAG GACTATATCA ACAGCCCCGT GCGCGATCTG AATGGCGTGT GCTTCGACTC
 4681 CCGGTTTCAG AACCCAGAGT GGCCCATGGA CGCCGATGCC AATGGCGCCT ACCACATCGC
 4741 CCTGAAGGGC CAGCTGCTGC TGAATCACCT GAAGGAGAGC AAGGATCTGA AGCTGCAGAA
 4801 CGGCATCTCC AATCAGGACT GGCTGGCCTA CATCCAGGAG CTGCGCAACA AAAGGCCGGC
 4861 GGCCACGAAA AAGGCCGGCC AGGCAAAAAA GAAAAAGGGA TCCTACCCAT ACGATGTTCC
 4921 AGATTACGCT TATCCCTACG ACGTGCCTGA TTATGCATAC CCATATGATG TCCCCGACTA
 4981 TGCCTAAGaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt
 5041 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
 5101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
 5161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
 5221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
 5281 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
 5341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
 5401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
 5461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
 5521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
 5581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
 5641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
 5701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
 5761 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
 5821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
 5881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
 5941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
 6001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
 6061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
 6121 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
 6181 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
 6241 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
 6301 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
 6361 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
 6421 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
 6481 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
 6541 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
 6601 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
 6661 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
 6721 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
 6781 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
 6841 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
 6901 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
 6961 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
 7021 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
 7081 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
 7141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
 7201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
 7261 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
 7321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
 7381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
 7441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
 7501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
 7561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
 7621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
 7681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
 7741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
 7801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
 7861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
 7921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
 7981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
 8041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
 8101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
 8161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
 8221 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
 8281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
 8341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
 8401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
 8461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
 8521 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
 8581 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
 8641 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
 8701 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
 8761 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
 8821 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
 8881 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
 8941 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
 9001 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
 9061 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
 9121 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
 9181 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
 9241 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
 9301 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
 9361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
 9421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//