RCIscript-GoldyTALEN

LOCUS       RCIscript-GoldyTALEN	4651 bp 	DNA	SYN
DEFINITION  RCIscript-GoldyTALEN
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="RCIscript-GoldyTALEN"
                     /mol_type="other DNA"
     promoter        146..165
                     /label="T3_promoter"
     CDS             270..911
                     /label="ORF frame 3"
     misc_feature    285..305
                     /label="SV40 NLS"
     misc_feature    291..305
                     /label="NLS"
     misc_feature    complement(767..922)
                     /label="lacZ_a"
     misc_feature    893..915
                     /label="M13_pUC_fwd_primer"
     promoter        908..924
                     /label="M13_forward20_primer"
     promoter        934..952
                     /label="T7_promoter"
     promoter        complement(999..1018)
                     /label="T3_promoter"
     promoter        complement(1035..1053)
                     /label="M13_reverse_primer"
     misc_feature    complement(1052..1074)
                     /label="M13_pUC_rev_primer"
     promoter        complement(1088..1117)
                     /label="lac_promoter"
     CDS             1132..1932
                     /label="ORF frame 1"
     promoter        complement(2207..2225)
                     /label="T7_promoter"
     misc_feature    2229..2384
                     /label="lacZ_a"
     promoter        complement(2232..2248)
                     /label="M13_forward20_primer"
     misc_feature    complement(2241..2263)
                     /label="M13_pUC_fwd_primer"
     rep_origin      complement(2521..2827)
                     /label="f1_origin"
     promoter        2906..2934
                     /label="AmpR_promoter"
     gene            2976..3836
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2976..3836
                     /label="ORF frame 3"
     rep_origin      3991..4610
                     /label="pBR322_origin"
ORIGIN
    1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
   61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
  121 ggaagagcgc ccaatacgca aaccgaatta accctcacta aagggaagct tgcttgttct
  181 ttttgcagaa gctcagaata aacgctcaac tttggcagat ctaactcgag aaagatattg
  241 tatatatcgt aacaatagga ggttcaacaa tggcttcctc ccctccaaag aaaaagagaa
  301 aggttagttg gaaggacgca agtggttggt ctagagtgga tctacgcacg ctcggctaca
  361 gtcagcagca gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg
  421 aggcactggt gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg
  481 cagcgttagg gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga
  541 cacacgaaga catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct
  601 tgctcacgga tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg
  661 tgaagattgc aaaacgtggc ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg
  721 cactgacggg tgcccccctg gagacgggcg ccgctacagg gcgcgtccca ttcgccattc
  781 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
  841 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
  901 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
  961 ggtaccgggc cccccctcga ggtcctccag cttttgttcc ctttagtgag ggttaattgc
 1021 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
 1081 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
 1141 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc caccggtcgt ctccaacgac
 1201 cacctcgtcg ccttggcctg cctcggcgga cgtcctgcca tggatgcagt gaaaaaggga
 1261 ttgccgcacg cgccggaatt gatcagaaga gtcaatcgcc gtattggcga acgcacgtcc
 1321 catcgcgttg cctctagatc ccagctagtg aaatctgaat tggaagagaa gaaatctgaa
 1381 cttagacata aattgaaata tgtgccacat gaatatattg aattgattga aatcgcaaga
 1441 aattcaactc aggatagaat ccttgaaatg aaggtgatgg agttctttat gaaggtttat
 1501 ggttatcgtg gtaaacattt gggtggatca aggaaaccag acggagcaat ttatactgtc
 1561 ggatctccta ttgattacgg tgtgatcgtt gatactaagg catattcagg aggttataat
 1621 cttccaattg gtcaagcaga tgaaatgcaa agatatgtcg aagagaatca aacaagaaac
 1681 aagcatatca accctaatga atggtggaaa gtctatccat cttcagtaac agaatttaag
 1741 ttcttgtttg tgagtggtca tttcaaagga aactacaaag ctcagcttac aagattgaat
 1801 catatcacta attgtaatgg agctgttctt agtgtagaag agcttttgat tggtggagaa
 1861 atgattaaag ctggtacatt gacacttgag gaagtgagaa ggaaatttaa taacggtgag
 1921 ataaactttt aataggctag tgactgacta ggatctggtt accactaaac cagcctcaag
 1981 aacacccgaa tggagtctct aagctacata ataccaactt acacttacaa aatgttgtcc
 2041 cccaaaatgt agccattcgt atctgctcct aataaaaaga aagtttcttc acattctaaa
 2101 aaaaaaaaaa aaaaaaaaaa aaaaaaaccc cccccccccc cccccccccc cccccccgca
 2161 tgcctgcagg tcgactagga tccccgggta ccgagctcca attcgcccta tagtgagtcg
 2221 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
 2281 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
 2341 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg
 2401 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
 2461 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
 2521 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
 2581 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
 2641 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
 2701 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
 2761 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
 2821 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
 2881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
 2941 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
 3001 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
 3061 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
 3121 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
 3181 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
 3241 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
 3301 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
 3361 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
 3421 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
 3481 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
 3541 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
 3601 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
 3661 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
 3721 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
 3781 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
 3841 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
 3901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
 3961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
 4021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
 4081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
 4141 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
 4201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
 4261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
 4321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
 4381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
 4441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
 4501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
 4561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
 4621 ggttcctggc cttttgctgg ccttttgctc a
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