EF.hICN1.Ubc.GFP

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: EF.hICN1.Ubc.GFP 
出品公司: 爱迪基因
目录编号: 17626
存储实验室: Linzhao Cheng

载体骨架基本信息
载体名称: cFUGW
载体抗性: 氨苄霉素
空载体大小: 11500 bp
载体类型: 哺乳细胞表达,慢病毒
筛选标记: GFP
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: ICN1
物种来源: H. sapiens (人类)
插入大小: 2400 bp
克隆方法: 限制性内切酶
5' 测序引物 EF-1a Forward

文献引用方法
材料和方法部分: EF.hICN1.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 17626)
参考文献部分: HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI. Stem Cells. 2006 Apr . 24(4):876-88. 10.1634/stemcells.2005-0598 PubMed 16513761

质粒图谱信息

EF.hICN1.Ubc.GFP载体图谱

质粒简介

质粒序列

LOCUS       EF.hICN1.Ubc.GFP	13830 bp 	DNA	SYN
DEFINITION  EF.hICN1.Ubc.GFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..13830
                     /organism="EF.hICN1.Ubc.GFP"
                     /mol_type="other DNA"
     promoter        239..815
                     /label="CMV_immearly_promoter"
     misc_feature    318..605
                     /label="CAG_enhancer"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    835..1015
                     /label="HIV-1_5_LTR"
     misc_feature    835..1015
                     /label="truncHIV-1_3_LTR"
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
     CDS             1558..2445
                     /label="ORF frame 1"
     misc_feature    1680..1913
                     /label="RRE"
     misc_feature    2444..2459
                     /label="cPPT"
     misc_feature    2623..2639
                     /label="pBluescriptKS_primer"
     promoter        2726..3909
                     /label="EF1a_promoter"
     CDS             complement(3029..3649)
                     /label="ORF frame 3"
     misc_feature    3857..3877
                     /label="EF1a_fwd_primer"
     promoter        3926..3944
                     /label="T7_promoter"
     CDS             3985..6375
                     /label="ORF frame 1"
     promoter        6507..7735
                     /label="hUbC_promoter"
     CDS             7762..8481
                     /label="ORF frame 1"
     gene            7765..8478
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(7807..7828)
                     /label="EGFP_N_primer"
     misc_feature    7945..7974
                     /label="Y66 (EGFP)"
     misc_feature    8415..8436
                     /label="EGFP_C_primer"
     misc_feature    8524..9111
                     /label="WPRE"
     CDS             8612..9661
                     /label="ORF frame 2"
     CDS             8625..9137
                     /label="ORF frame 3"
     misc_feature    complement(9114..9130)
                     /label="pBluescriptKS_primer"
     misc_feature    9298..9313
                     /label="cPPT"
     misc_feature    9298..9319
                     /label="U3PPT"
     misc_feature    9635..9815
                     /label="truncHIV-1_3_LTR"
     misc_feature    9635..9815
                     /label="HIV-1_5_LTR"
     misc_feature    complement(9841..9858)
                     /label="BGH_rev_primer"
     terminator      9844..10071
                     /label="bGH_PA_terminator"
     rep_origin      10134..10440
                     /label="f1_origin"
     misc_feature    complement(10554..10574)
                     /label="pBABE_3_primer"
     misc_feature    complement(10560..10775)
                     /label="SV40_enhancer"
     promoter        10572..10840
                     /label="SV40_promoter"
     rep_origin      10739..10816
                     /label="SV40_origin"
     misc_feature    10801..10820
                     /label="SV40pro_F_primer"
     promoter        10934..11001
                     /label="EM7_promoter"
     misc_feature    11002..11376
                     /label="sh_ble"
     gene            11002..11373
                     /label="bleo"
                     /gene="bleo"
     terminator      11509..11628
                     /label="SV40_PA_terminator"
     misc_feature    11597..11616
                     /label="EBV_rev_primer"
     promoter        complement(11672..11690)
                     /label="M13_reverse_primer"
     misc_feature    complement(11689..11711)
                     /label="M13_pUC_rev_primer"
     promoter        complement(11725..11754)
                     /label="lac_promoter"
     gene            complement(12837..13697)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(12837..13697)
                     /label="ORF frame 2"
     promoter        complement(13739..13767)
                     /label="AmpR_promoter"
ORIGIN
    1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
   61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
  121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
  181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
  241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
  301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
  361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
  421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
  481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
  541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
  601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
  661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
  721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
  781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT
  841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT
  901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC
  961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC
 1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC
 1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA
 1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG
 1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA
 1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC
 1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA
 1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC
 1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA
 1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG
 1561 AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA
 1621 GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA
 1681 GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG
 1741 ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG
 1801 CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG
 1861 CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT
 1921 TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT
 1981 AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT
 2041 AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG
 2101 AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA
 2161 ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA
 2221 AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA
 2281 TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA
 2341 GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT
 2401 GCGCCAATTC TGCAGACAAA TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT
 2461 TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA
 2521 AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG GTTTATTACA GGGACAGCAG
 2581 AGATCCAGTT TGGTTAATTA AgtttaaacG GGCCCTCTAG ActcgagGTC GACGGtATCG
 2641 ATAAGCTTTG CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA GCTAATGGAC
 2701 CTTCTAGGTC TTGAAAGGAG TGCCTCGTGA GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC
 2761 ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG
 2821 AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC
 2881 GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC
 2941 GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT
 3001 ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT
 3061 GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC
 3121 CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC
 3181 TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT
 3241 TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT
 3301 CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG
 3361 CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG
 3421 TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC
 3481 TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC
 3541 GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG
 3601 TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG
 3661 GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT
 3721 TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT
 3781 GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT
 3841 GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG
 3901 GTGTCGTGAG GAATTAGCTT GGTACTAATA CGACTCACTA TAGGGAGACC CAAGCTGGCT
 3961 AGTTAAGCTT GATgatccgg aggCatgcgg cggcagcatg gccagctctg gttccctgag
 4021 ggcttcaaag tgtctgaggc cagcaagaag aagcggcggg agcccctcgg cgaggactcc
 4081 gtgggcctca agcccctgaa gaacgcttca gacggtgccc tcatggacga caaccagaat
 4141 gagtgggggg acgaggacct ggagaccaag aagttccggt tcgaggagcc cgtggttctg
 4201 cctgacctgg acgaccagac agaccaccgg cagtggactc agcagcacct ggatgccgct
 4261 gacctgcgca tgtctgccat ggcccccaca ccgccccagg gtgaggttga cgccgactgc
 4321 atggacgtca atgtccgcgg gcctgatggc ttcaccccgc tcatgatcgc ctcctgcagc
 4381 gggggcggcc tggagacggg caacagcgag gaagaggagg acgcgccggc cgtcatctcc
 4441 gacttcatct accagggcgc cagcctgcac aaccagacag accgcacggg cgagaccgcc
 4501 ttgcacctgg ccgcccgcta ctcacgctct gatgccgcca agcgcctgct ggaggccagc
 4561 gcagatgcca acatccagga caacatgggc cgcaccccgc tgcatgcggc tgtgtctgcc
 4621 gacgcacaag gtgtcttcca gatcctgatc cggaaccgag ccacagacct ggatgcccgc
 4681 atgcatgatg gcacgacgcc actgatcctg gctgcccgcc tggccgtgga gggcatgctg
 4741 gaggacctca tcaactcaca cgccgacgtc aacgccgtag atgacctggg caagtccgcc
 4801 ctgcactggg ccgccgccgt gaacaatgtg gatgccgcag ttgtgctcct gaagaacggg
 4861 gctaacaaag atatgcagaa caacagggag gagacacccc tgtttctggc cgcccgggag
 4921 ggcagctacg agaccgccaa ggtgctgctg gaccactttg ccaaccggga catcacggat
 4981 catatggacc gcctgccgcg cgacatcgca caggagcgca tgcatcacga catcgtgagg
 5041 ctgctggacg agtacaacct ggtgcgcagc ccgcagctgc acggagcccc gctggggggc
 5101 acgcccaccc tgtcgccccc gctctgctcg cccaacggct acctgggcag cctcaagccc
 5161 ggcgtgcagg gcaagaaggt ccgcaagccc agcagcaaag gcctggcctg tggaagcaag
 5221 gaggccaagg acctcaaggc acggaggaag aagtcccagg atggcaaggg ctgcctgctg
 5281 gacagctccg gcatgctctc gcccgtggac tccctggagt caccccatgg ctacctgtca
 5341 gacgtggcct cgccgccact gctgccctcc ccgttccagc agtctccgtc cgtgcccctc
 5401 aaccacctgc ctgggatgcc cgacacccac ctgggcatcg ggcacctgaa cgtggcggcc
 5461 aagcccgaga tggcggcgct gggtgggggc ggccggctgg cctttgagac tggcccacct
 5521 cgtctctccc acctgcctgt ggcctctggc accagcaccg tcctgggctc cagcagcgga
 5581 ggggccctga atttcactgt gggcgggtcc accagtttga atggtcaatg cgagtggctg
 5641 tcccggctgc agagcggcat ggtgccgaac caatacaacc ctctgcgggg gagtgtggca
 5701 ccaggccccc tgagcacaca ggccccctcc ctgcagcatg gcatggtagg cccgctgcac
 5761 agtagccttg ctgccagcgc cctgtcccag atgatgagct accagggcct gcccagcacc
 5821 cggctggcca cccagcctca cctggtgcag acccagcagg tgcagccaca aaacttacag
 5881 atgcagcagc agaacctgca gccagcaaac atccagcagc agcaaagcct gcagccgcca
 5941 ccaccaccac cacagccgca ccttggcgtg agctcagcag ccagcggcca cctgggccgg
 6001 agcttcctga gtggagagcc gagccaggca gacgtgcagc cactgggccc cagcagcctg
 6061 gcggtgcaca ctattctgcc ccaggagagc cccgccctgc ccacgtcgct gccatcctcg
 6121 ctggtcccac ccgtgaccgc agcccagttc ctgacgcccc cctcgcagca cagctactcc
 6181 tcgcctgtgg acaacacccc cagccaccag ctacaggtgc ctgagcaccc cttcctgacc
 6241 ccttcgccgg agtcgcccga ccaatggtcg tcctcgtcgc cgcactctaa tgtgtctgac
 6301 tggtctgagg gcgtgtcgtc gcccccgacc tccatgcagt cccagatcgc gcgcatcccg
 6361 gaggcgttca agTAAacggc gcgtcagatc cactagtaac ggccgccagt gtgctggaat
 6421 tATCGAATTC Ccaccacact ggactagtGG ATCCgggggt accgcgtcga gTTAATTAAg
 6481 ggcacagccg aggtctagag gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
 6541 ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca ggagcgttcc tgatccttcc
 6601 gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat
 6661 cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca
 6721 gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg
 6781 tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg
 6841 tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg
 6901 agttgcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg
 6961 aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa
 7021 ctgggggttg gggggagcgc acaaaatggc ggctgttccc gagtcttgaa tggaagacgc
 7081 ttgtaaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
 7141 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
 7201 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg ggtttgtcgt
 7261 ctggttgcgg gggcggcagt tatgcggtgc cgttgggcag tgcacccgta cctttgggag
 7321 cgcgcgcctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
 7381 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
 7441 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
 7501 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
 7561 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
 7621 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaagc ttctgcaggt
 7681 cgactctaga aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacaGGAT
 7741 CCCCGGGTAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG
 7801 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG
 7861 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG
 7921 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC
 7981 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC
 8041 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG
 8101 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG
 8161 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC
 8221 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG
 8281 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
 8341 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC
 8401 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
 8461 ATGGACGAGC TGTACAAGTA AAGCGGCCGC GACTCTAGAA TTCGATATCA AGCTTATCGA
 8521 TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC
 8581 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG
 8641 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT
 8701 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC
 8761 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC
 8821 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT
 8881 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT
 8941 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT
 9001 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT
 9061 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG
 9121 TCGACCTCGA GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA
 9181 TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC
 9241 TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA
 9301 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT
 9361 GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG
 9421 GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA
 9481 GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG
 9541 GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA
 9601 TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG
 9661 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC
 9721 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT
 9781 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGGGCC CGTTTAAACC CGCTGATCAG
 9841 CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT
 9901 TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC
 9961 ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG
10021 AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG
10081 CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA
10141 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC
10201 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG
10261 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA
10321 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC
10381 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA
10441 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT
10501 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT
10561 GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT
10621 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG
10681 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT
10741 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
10801 TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG
10861 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG
10921 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC
10981 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG
11041 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC
11101 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC
11161 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG
11221 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC
11281 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC
11341 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC
11401 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG
11461 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA
11521 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
11581 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA
11641 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
11701 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
11761 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG
11821 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
11881 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
11941 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
12001 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
12061 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
12121 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
12181 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
12241 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
12301 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
12361 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
12421 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
12481 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
12541 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
12601 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
12661 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
12721 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
12781 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
12841 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
12901 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
12961 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
13021 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
13081 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
13141 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
13201 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
13261 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
13321 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
13381 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
13441 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
13501 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
13561 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
13621 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
13681 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
13741 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
13801 CGCACATTTC CCCGAAAAGT GCCACCTGAC
//

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