pMD2.G

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: pMD2.G 
出品公司: 爱迪基因
目录编号: 12259
存储实验室: Didier Trono

载体骨架基本信息
载体名称: pMD2.G
载体抗性: 氨苄霉素
空载体大小: 5824 bp
载体类型: 哺乳细胞表达,慢病毒,抗原
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: VSV G
克隆方法: 限制性内切酶
5' 测序引物 CMV-F

文献引用方法
材料和方法部分: pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259)

质粒图谱信息

pMD2.G载体图谱

质粒简介

质粒序列

LOCUS       pMD2.G Sequencing Result	5822 bp 	DNA	SYN
DEFINITION  pMD2.G Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="pMD2.G Sequencing Result"
                     /mol_type="other DNA"
     gene            8..835
                     /label="vsv-G (696 - 1523)"
                     /gene="vsv-G (696 - 1523)"
     CDS             20..847
                     /label="ORF frame 2"
     misc_feature    812..844
                     /label="VSVG Tag"
     gene            complement(2711..3571)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2711..3571)
                     /label="ORF frame 2"
     promoter        complement(3613..3641)
                     /label="AmpR_promoter"
     misc_feature    complement(3746..3765)
                     /label="SV40pro_F_primer"
     promoter        3868..4444
                     /label="CMV_immearly_promoter"
     misc_feature    3947..4234
                     /label="CAG_enhancer"
     misc_feature    4401..4421
                     /label="CMV_fwd_primer"
     promoter        4402..4471
                     /label="CMV_promoter"
     promoter        4414..4533
                     /label="CMV2_promoter"
     misc_feature    4445..4464
                     /label="pCEP_fwd_primer"
     misc_feature    4447..4471
                     /label="LNCX_primer"
     misc_feature    4721..5104
                     /label="bGlob_int"
     gene            5134..835
                     /label="vsv-G"
                     /gene="vsv-G"
     CDS             5134..847
                     /label="ORF frame 1"
ORIGIN
    1 TGGAGGCAAG GCCTGCAAAA TGCAATACTG CAAGCATTGG GGAGTCAGAC TCCCATCAGG
   61 TGTCTGGTTC GAGATGGCTG ATAAGGATCT CTTTGCTGCA GCCAGATTCC CTGAATGCCC
  121 AGAAGGGTCA AGTATCTCTG CTCCATCTCA GACCTCAGTG GATGTAAGTC TAATTCAGGA
  181 CGTTGAGAGG ATCTTGGATT ATTCCCTCTG CCAAGAAACC TGGAGCAAAA TCAGAGCGGG
  241 TCTTCCAATC TCTCCAGTGG ATCTCAGCTA TCTTGCTCCT AAAAACCCAG GAACCGGTCC
  301 TGCTTTCACC ATAATCAATG GTACCCTAAA ATACTTTGAG ACCAGATACA TCAGAGTCGA
  361 TATTGCTGCT CCAATCCTCT CAAGAATGGT CGGAATGATC AGTGGAACTA CCACAGAAAG
  421 GGAACTGTGG GATGACTGGG CACCATATGA AGACGTGGAA ATTGGACCCA ATGGAGTTCT
  481 GAGGACCAGT TCAGGATATA AGTTTCCTTT ATACATGATT GGACATGGTA TGTTGGACTC
  541 CGATCTTCAT CTTAGCTCAA AGGCTCAGGT GTTCGAACAT CCTCACATTC AAGACGCTGC
  601 TTCGCAACTT CCTGATGATG AGAGTTTATT TTTTGGTGAT ACTGGGCTAT CCAAAAATCC
  661 AATCGAGCTT GTAGAAGGTT GGTTCAGTAG TTGGAAAAGC TCTATTGCCT CTTTTTTCTT
  721 TATCATAGGG TTAATCATTG GACTATTCTT GGTTCTCCGA GTTGGTATCC ATCTTTGCAT
  781 TAAATTAAAG CACACCAAGA AAAGACAGAT TTATACAGAC ATAGAGATGA ACCGACTTGG
  841 AAAGTAACTC AAATCCTGCA CAACAGATTC TTCATGTTTG GACCAAATCA ACTTGTGATA
  901 CCATGCTCAA AGAGGCCTCA ATTATATTTG AGTTTTTAAT TTTTATGAAA AAAAAAAAAA
  961 AAAACGGAAT TCACCCCACC AGTGCAGGCT GCCTATCAGA AAGTGGTGGC TGGTGTGGCT
 1021 AATGCCCTGG CCCACAAGTA TCACTAAGCT CGCTTTCTTG CTGTCCAATT TCTATTAAAG
 1081 GTTCCTTTGT TCCCTAAGTC CAACTACTAA ACTGGGGGAT ATTATGAAGG GCCTTGAGCA
 1141 TCTGGATTCT GCCTAATAAA AAACATTTAT TTTCATTGCA ATGATGTATT TAAATTATTT
 1201 CTGAATATTT TACTAAAAAG GGAATGTGGG AGGTCAGTGC ATTTAAAACA TAAAGAAATG
 1261 AAGAGCTAGT TCAAACCTTG GGAAAATACA CTATATCTTA AACTCCATGA AAGAAGGTGA
 1321 GGCTGCAAAC AGCTAATGCA CATTGGCAAC AGCCCCTGAT GCCTATGCCT TATTCATCCC
 1381 TCAGAAAAGG ATTCAAGTAG AGGCTTGATT TGGAGGTTAA AGTTTTGCTA TGCTGTATTT
 1441 TACATTACTT ATTGTTTTAG CTGTCCTCAT GAATGTCTTT TCACTACCCA TTTGCTTATC
 1501 CTGCATCTCT CAGCCTTGAC TCCACTCAGT TCTCTTGCTT AGAGATACCA CCTTTCCCCT
 1561 GAAGTGTTCC TTCCATGTTT TACGGCGAGA TGGTTTCTCC TCGCCTGGCC ACTCAGCCTT
 1621 AGTTGTCTCT GTTGTCTTAT AGAGGTCTAC TTGAAGAAGG AAAAACAGGG GGCATGGTTT
 1681 GACTGTCCTG TGAGCCCTTC TTCCCTGCCT CCCCCACTCA CAGTGACCCG GAATCCCTCG
 1741 ACATGGCAGT CTAGCACTAG TGCGGCCGCA GATCTGCTTC CTCGCTCACT GACTCGCTGC
 1801 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
 1861 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
 1921 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
 1981 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
 2041 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
 2101 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
 2161 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
 2221 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
 2281 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
 2341 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT
 2401 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
 2461 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
 2521 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT
 2581 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT
 2641 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
 2701 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
 2761 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC
 2821 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT
 2881 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG
 2941 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
 3001 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
 3061 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT
 3121 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG
 3181 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA
 3241 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
 3301 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT
 3361 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC
 3421 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA
 3481 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA
 3541 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA
 3601 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
 3661 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTGGAT CCCCTGAGGG
 3721 GGCCCCCATG GGCTAGAGGA TCCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG
 3781 CCATGAGCTT GGCCCATTGC ATACGTTGTA TCCATATCAT AATATGTACA TTTATATTGG
 3841 CTCATGTCCA ACATTACCGC CATGTTGACA TTGATTATTG ACTAGTTATT AATAGTAATC
 3901 AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT
 3961 AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA
 4021 TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG
 4081 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA
 4141 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT
 4201 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG
 4261 GCAGTACATC AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC
 4321 CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG
 4381 TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
 4441 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
 4501 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCCCTCG AAGCTTACAT GTGGTACCGA
 4561 GCTCGGATCC TGAGAACTTC AGGGTGAGTC TATGGGACCC TTGATGTTTT CTTTCCCCTT
 4621 CTTTTCTATG GTTAAGTTCA TGTCATAGGA AGGGGAGAAG TAACAGGGTA CACATATTGA
 4681 CCAAATCAGG GTAATTTTGC ATTTGTAATT TTAAAAAATG CTTTCTTCTT TTAATATACT
 4741 TTTTTGTTTA TCTTATTTCT AATACTTTCC CTAATCTCTT TCTTTCAGGG CAATAATGAT
 4801 ACAATGTATC ATGCCTCTTT GCACCATTCT AAAGAATAAC AGTGATAATT TCTGGGTTAA
 4861 GGCAATAGCA ATATTTCTGC ATATAAATAT TTCTGCATAT AAATTGTAAC TGATGTAAGA
 4921 GGTTTCATAT TGCTAATAGC AGCTACAATC CAGCTACCAT TCTGCTTTTA TTTTATGGTT
 4981 GGGATAAGGC TGGATTATTC TGAGTCCAAG CTAGGCCCTT TTGCTAATCA TGTTCATACC
 5041 TCTTATCTTC CTCCCACAGC TCCTGGGCAA CGTGCTGGTC TGTGTGCTGG CCCATCACTT
 5101 TGGCAAAGCA CGTGAGATCT GAATTCTGAC ACTATGAAGT GCCTTTTGTA CTTAGCCTTT
 5161 TTATTCATTG GGGTGAATTG CAAGTTCACC ATAGTTTTTC CACACAACCA AAAAGGAAAC
 5221 TGGAAAAATG TTCCTTCTAA TTACCATTAT TGCCCGTCAA GCTCAGATTT AAATTGGCAT
 5281 AATGACTTAA TAGGCACAGC CTTACAAGTC AAAATGCCCA AGAGTCACAA GGCTATTCAA
 5341 GCAGACGGTT GGATGTGTCA TGCTTCCAAA TGGGTCACTA CTTGTGATTT CCGCTGGTAT
 5401 GGACCGAAGT ATATAACACA TTCCATCCGA TCCTTCACTC CATCTGTAGA ACAATGCAAG
 5461 GAAAGCATTG AACAAACGAA ACAAGGAACT TGGCTGAATC CAGGCTTCCC TCCTCAAAGT
 5521 TGTGGATATG CAACTGTGAC GGATGCCGAA GCAGTGATTG TCCAGGTGAC TCCTCACCAT
 5581 GTGCTGGTTG ATGAATACAC AGGAGAATGG GTTGATTCAC AGTTCATCAA CGGAAAATGC
 5641 AGCAATTACA TATGCCCCAC TGTCCATAAC TCTACAACCT GGCATTCTGA CTATAAGGTC
 5701 AAAGGGCTAT GTGATTCTAA CCTCATTTCC ATGGACATCA CCTTCTTCTC AGAGGACGGA
 5761 GAGCTATCAT CCCTGGGAAA GGAGGGCACA GGGTTCAGAA GTAACTACTT TGCTTATGAA
 5821 AC
//

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