pHIV-H2BmRFP

LOCUS       pHIV-H2BmRFP	8045 bp 	DNA	SYN
DEFINITION  pHIV-H2BmRFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="pHIV-H2BmRFP"
                     /mol_type="other DNA"
     promoter        239..815
                     /label="CMV_immearly_promoter"
     misc_feature    318..605
                     /label="CAG_enhancer"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    835..1015
                     /label="HIV-1_5_LTR"
     misc_feature    835..1015
                     /label="truncHIV-1_3_LTR"
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
     CDS             1564..2451
                     /label="ORF frame 1"
     misc_feature    1686..1919
                     /label="RRE"
     misc_feature    2450..2465
                     /label="cPPT"
     promoter        2622..3805
                     /label="EF1a_promoter"
     CDS             complement(2925..3545)
                     /label="ORF frame 1"
     misc_feature    3753..3773
                     /label="EF1a_fwd_primer"
     misc_feature    3850..4434
                     /label="IRES"
     CDS             4426..5514
                     /label="ORF frame 1"
     gene            4840..5511
                     /label="mRFP1"
                     /gene="mRFP1"
     misc_feature    5413..5439
                     /label="I197 (mTangerine)"
     misc_feature    5429..5452
                     /label="dsRed1_C_primer"
     misc_feature    complement(5526..5542)
                     /label="pBluescriptKS_primer"
     misc_feature    5714..5729
                     /label="cPPT"
     misc_feature    5714..5735
                     /label="U3PPT"
     misc_feature    6051..6231
                     /label="truncHIV-1_3_LTR"
     misc_feature    6051..6231
                     /label="HIV-1_5_LTR"
     gene            complement(7052..7912)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(7052..7912)
                     /label="ORF frame 2"
     promoter        complement(7954..7982)
                     /label="AmpR_promoter"
ORIGIN
    1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
   61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
  121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
  181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
  241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
  301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
  361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
  421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
  481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
  541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
  601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
  661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
  721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
  781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT
  841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT
  901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC
  961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC
 1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC
 1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA
 1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG
 1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA
 1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC
 1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA
 1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC
 1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA
 1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG GCCGCGCTGA TCTTCAGACC TGGAGGAGGA
 1561 GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA
 1621 TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG
 1681 GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG
 1741 TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC
 1801 AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC
 1861 AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG
 1921 GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT
 1981 TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA
 2041 GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG AAGAATCGCA AAACCAGCAA
 2101 GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG CAAGTTTGTG GAATTGGTTT
 2161 AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA TGATAGTAGG AGGCTTGGTA
 2221 GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA GAGTTAGGCA GGGATATTCA
 2281 CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC CCGACAGGCC CGAAGGAATA
 2341 GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC GATTAGTGAA CGGATCGGCA
 2401 CTGCGTGCGC CAATTCTGCA GACAAATGGC AGTATTCATC CACAATTTTA AAAGAAAAGG
 2461 GGGGATTGGG GGGTACAGTG CAGGGGAAAG AATAGTAGAC ATAATAGCAA CAGACATACA
 2521 AACTAAAGAA TTACAAAAAC AAATTACAAA AATTCAAAAT TTTCGGGTTT ATTACAGGGA
 2581 CAGCAGAGAT CCAGTTTGGT TAGTACCGGG CCCGCTCTAG CCGTGAGGCT CCGGTGCCCG
 2641 TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA
 2701 TTGAACCGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG
 2761 GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA
 2821 CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGGTA AGTGCCGTGT GTGGTTCCCG
 2881 CGGGCCTGGC CTCTTTACGG GTTATGGCCC TTGCGTGCCT TGAATTACTT CCACCTGGCT
 2941 GCAGTACGTG ATTCTTGATC CCGAGCTTCG GGTTGGAAGT GGGTGGGAGA GTTCGAGGCC
 3001 TTGCGCTTAA GGAGCCCCTT CGCCTCGTGC TTGAGTTGAG GCCTGGCCTG GGCGCTGGGG
 3061 CCGCCGCGTG CGAATCTGGT GGCACCTTCG CGCCTGTCTC GCTGCTTTCG ATAAGTCTCT
 3121 AGCCATTTAA AATTTTTGAT GACCTGCTGC GACGCTTTTT TTCTGGCAAG ATAGTCTTGT
 3181 AAATGCGGGC CAAGATCTGC ACACTGGTAT TTCGGTTTTT GGGGCCGCGG GCGGCGACGG
 3241 GGCCCGTGCG TCCCAGCGCA CATGTTCGGC GAGGCGGGGC CTGCGAGCGC GGCCACCGAG
 3301 AATCGGACGG GGGTAGTCTC AAGCTGGCCG GCCTGCTCTG GTGCCTGGCC TCGCGCCGCC
 3361 GTGTATCGCC CCGCCCTGGG CGGCAAGGCT GGCCCGGTCG GCACCAGTTG CGTGAGCGGA
 3421 AAGATGGCCG CTTCCCGGCC CTGCTGCAGG GAGCTCAAAA TGGAGGACGC GGCGCTCGGG
 3481 AGAGCGGGCG GGTGAGTCAC CCACACAAAG GAAAAGGGCC TTTCCGTCCT CAGCCGTCGC
 3541 TTCATGTGAC TCCACGGAGT ACCGGGCGCC GTCCAGGCAC CTCGATTAGT TCTCGAGCTT
 3601 TTGGAGTACG TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC
 3661 TGAGTGGGTG GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT
 3721 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT
 3781 TTTTCTTCCA TTTCAGGTGT CGTGAGCGGC CGCTGAGTTA ACTATTCTAG AGTACCCGGG
 3841 CTAGGATCCG CCCCTCTCCC TCCCCCCCCC CTAACGTTAC TGGCCGAAGC CGCTTGGAAT
 3901 AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT ATTGCCGTCT TTTGGCAATG
 3961 TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT TCCTAGGGGT CTTTCCCCTC
 4021 TCGCCAAAGG AATGCAAGGT CTGTTGAATG TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT
 4081 CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA GCGGAACCCC CCACCTGGCG
 4141 ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC ACCTGCAAAG GCGGCACAAC
 4201 CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT CAAATGGCTC TCCTCAAGCG
 4261 TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA TTGTATGGGA TCTGATCTGG
 4321 GGCCTCGGTG CACATGCTTT ACATGTGTTT AGTCGAGGTT AAAAAAACGT CTAGGCCCCC
 4381 CGAACCACGG GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG
 4441 TCACCAGAGC CAGCGAAGTC TGCTCCCGCC CCGAAAAAGG GCTCCAAGAA GGCGGTGACT
 4501 AAGGCGCAGA AGAAAGGCGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTATTCCATC
 4561 TATGTGTACA AGGTTCTGAA GCAGGTCCAC CCTGACACCG GCATTTCGTC CAAGGCCATG
 4621 GGCATCATGA ATTCGTTTGT GAACGACATT TTCGAGCGCA TCGCAGGTGA GGCTTCCCGC
 4681 CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC
 4741 CTGCTGCTGC CTGGGGAGTT GGCCAAGCAC GCCGTGTCCG AGGGTACTAA GGCCATCACC
 4801 AAGTACACCA GCGCTAAGGA TCCACCGGTC GCCACCATGG CCTCCTCCGA GGACGTCATC
 4861 AAGGAGTTCA TGCGCTTCAA GGTGCGCATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG
 4921 ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG
 4981 ACCAAGGGCG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CCAGTACGGC
 5041 TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC
 5101 GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC
 5161 CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC
 5221 TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCACCGAG
 5281 CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGATGAGGCT GAAGCTGAAG
 5341 GACGGCGGCC ACTACGACGC CGAGGTCAAG ACCACCTACA TGGCCAAGAA GCCCGTGCAG
 5401 CTGCCCGGCG CCTACAAGAC CGACATCAAG CTGGACATCA CCTCCCACAA CGAGGACTAC
 5461 ACCATCGTGG AACAGTACGA GCGCGCCGAG GGCCGCCACT CCACCGGCGC CTAAGCGGCC
 5521 GCATCGATAC CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA
 5581 ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG
 5641 GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC
 5701 TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC
 5761 AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT
 5821 ACACACCAGG GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC
 5881 CAGTTGAGCA AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC
 5941 CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA
 6001 GCCGCCTAGC ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT
 6061 GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC
 6121 CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG
 6181 GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG
 6241 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
 6301 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
 6361 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
 6421 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
 6481 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
 6541 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
 6601 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
 6661 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
 6721 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
 6781 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
 6841 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
 6901 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
 6961 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
 7021 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
 7081 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
 7141 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
 7201 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
 7261 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
 7321 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
 7381 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
 7441 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
 7501 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
 7561 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
 7621 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
 7681 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
 7741 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
 7801 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
 7861 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
 7921 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
 7981 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
 8041 CTGAC
//