pHR-SFFV-dCas9-BFP-KRAB

价格:1200元

联系方式:I47-825O-882O

买家导航

质粒基本信息

质粒名称: pHR-SFFV-dCas9-BFP-KRAB 
出品公司: 爱迪基因
目录编号: 46911
存储实验室: Stanley Qi, Jonathan Weissman

载体骨架基本信息
载体名称: pHR
载体出品公司: Addgene
载体抗性: 氨苄霉素
空载体大小: 9000 bp
完整质粒大小: 14167 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: dCas9-BFP-KRAB fusion
物种来源: H. sapiens (human)
插入大小: 5172 bp
启动子 SFFV
标签/融合蛋白: HA(C端)     2xNLS(C端)     BFP(C端)      KRAB domain(C端)
克隆方法: Ligation Independent Cloning
5' 测序引物 gcttcccgagctctataaaagag
3' 测序引物 CCAGAGGTTGATTATCGATAAGC

文献引用方法
材料和方法部分: pHR-SFFV-dCas9-BFP-KRAB was a gift from Stanley Qi & Jonathan Weissman (Addgene plasmid # 46911)
参考文献部分: CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. 10.1016/j.cell.2013.06.044 PubMed 23849981

质粒图谱信息

pHR-SFFV-dCas9-BFP-KRAB载体图谱

质粒简介

质粒序列

LOCUS       pHR-SFFV-dCas9-BFP-KRAB	14167 bp 	DNA	SYN
DEFINITION  pHR-SFFV-dCas9-BFP-KRAB
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..14167
                     /organism="pHR-SFFV-dCas9-BFP-KRAB"
                     /mol_type="other DNA"
     misc_feature    454..634
                     /label="HIV-1_5_LTR"
     misc_feature    454..634
                     /label="truncHIV-1_3_LTR"
     misc_feature    745..789
                     /label="HIV-1_psi_pack"
     CDS             complement(1015..1476)
                     /label="ORF frame 2"
     misc_feature    1303..1535
                     /label="RRE"
     CDS             1642..2241
                     /label="ORF frame 1"
     misc_feature    2053..2068
                     /label="cPPT"
     CDS             complement(2675..7543)
                     /label="ORF frame 1"
     CDS             2801..7969
                     /label="ORF frame 2"
     misc_feature    6908..6934
                     /label="HA"
     misc_feature    6953..6973
                     /label="SV40 NLS"
     misc_feature    6959..6973
                     /label="NLS"
     misc_feature    8032..8619
                     /label="WPRE"
     CDS             8120..8647
                     /label="ORF frame 2"
     CDS             8133..8807
                     /label="ORF frame 3"
     misc_feature    complement(8622..8638)
                     /label="pBluescriptKS_primer"
     misc_feature    8711..8726
                     /label="cPPT"
     misc_feature    8711..8732
                     /label="U3PPT"
     misc_feature    8728..8780
                     /label="delta_U3"
     misc_feature    8781..8961
                     /label="HIV-1_5_LTR"
     misc_feature    8781..8961
                     /label="truncHIV-1_3_LTR"
     misc_feature    complement(8987..9004)
                     /label="Sp6_primer"
     gene            9242..9505
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(9294..9313)
                     /label="pBRrevBam_primer"
     misc_feature    9646..9668
                     /label="pGEX_3_primer"
     promoter        9827..9855
                     /label="AmpR_promoter"
     gene            9897..10757
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             9897..10757
                     /label="ORF frame 3"
     rep_origin      10912..11531
                     /label="pBR322_origin"
     misc_feature    complement(11757..11777)
                     /label="pBABE_3_primer"
     misc_feature    complement(11763..12216)
                     /label="SV40_enhancer"
     promoter        11775..12043
                     /label="SV40_promoter"
     rep_origin      11942..12019
                     /label="SV40_origin"
     misc_feature    12004..12023
                     /label="SV40pro_F_primer"
     misc_feature    13224..13239
                     /label="SV40_int"
     misc_feature    13245..13292
                     /label="SV40_3_splice"
     CDS             13369..13842
                     /label="ORF frame 1"
     misc_feature    13420..13440
                     /label="SV40 NLS"
     misc_feature    13426..13440
                     /label="NLS"
     terminator      13868..13999
                     /label="SV40_PA_terminator"
     misc_feature    13956..13975
                     /label="EBV_rev_primer"
ORIGIN
    1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
   61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
  121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
  181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
  241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
  301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
  361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
  421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
  481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
  541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
  601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
  661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
  721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
  781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
  841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
  901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
  961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
 1501 ggctgtgaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
 2041 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
 2101 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
 2161 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
 2221 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
 2281 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
 2341 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
 2401 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
 2461 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
 2521 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
 2581 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
 2641 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
 2701 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc
 2761 tgcaggatat caagcttgcg gtaccgcggg cccgGCCACC ATGGACAAGA AGTACAGCAT
 2821 CGGCCTGGCC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG ATCACCGACG AGTACAAGGT
 2881 GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG CACAGCATCA AGAAGAACCT
 2941 GATCGGCGCC CTGCTGTTCG ACAGCGGAGA AACAGCCGAG GCCACCCGGC TGAAGAGAAC
 3001 CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC TATCTGCAAG AGATCTTCAG
 3061 CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA CTGGAAGAGT CCTTCCTGGT
 3121 GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC AACATCGTGG ACGAGGTGGC
 3181 CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG AAACTGGTGG ACAGCACCGA
 3241 CAAGGCCGAC CTGCGGCTGA TCTATCTGGC CCTGGCCCAC ATGATCAAGT TCCGGGGCCA
 3301 CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC GTGGACAAGC TGTTCATCCA
 3361 GCTGGTGCAG ACCTACAACC AGCTGTTCGA GGAAAACCCC ATCAACGCCA GCGGCGTGGA
 3421 CGCCAAGGCC ATCCTGTCTG CCAGACTGAG CAAGAGCAGA CGGCTGGAAA ATCTGATCGC
 3481 CCAGCTGCCC GGCGAGAAGA AGAATGGCCT GTTCGGCAAC CTGATTGCCC TGAGCCTGGG
 3541 CCTGACCCCC AACTTCAAGA GCAACTTCGA CCTGGCCGAG GATGCCAAAC TGCAGCTGAG
 3601 CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC CAGATCGGCG ACCAGTACGC
 3661 CGACCTGTTT CTGGCCGCCA AGAACCTGTC CGACGCCATC CTGCTGAGCG ACATCCTGAG
 3721 AGTGAACACC GAGATCACCA AGGCCCCCCT GAGCGCCTCT ATGATCAAGA GATACGACGA
 3781 GCACCACCAG GACCTGACCC TGCTGAAAGC TCTCGTGCGG CAGCAGCTGC CTGAGAAGTA
 3841 CAAAGAGATT TTCTTCGACC AGAGCAAGAA CGGCTACGCC GGCTACATCG ATGGCGGAGC
 3901 CAGCCAGGAA GAGTTCTACA AGTTCATCAA GCCCATCCTG GAAAAGATGG ACGGCACCGA
 3961 GGAACTGCTC GTGAAGCTGA ACAGAGAGGA CCTGCTGCGG AAGCAGCGGA CCTTCGACAA
 4021 CGGCAGCATC CCCCACCAGA TCCACCTGGG AGAGCTGCAC GCCATTCTGC GGCGGCAGGA
 4081 AGATTTTTAC CCATTCCTGA AGGACAACCG GGAAAAGATC GAGAAGATCC TGACCTTCCG
 4141 CATCCCCTAC TACGTGGGCC CTCTGGCCAG GGGAAACAGC AGATTCGCCT GGATGACCAG
 4201 AAAGAGCGAG GAAACCATCA CCCCCTGGAA CTTCGAGGAA GTGGTGGACA AGGGCGCCAG
 4261 CGCCCAGAGC TTCATCGAGC GGATGACCAA CTTCGATAAG AACCTGCCCA ACGAGAAGGT
 4321 GCTGCCCAAG CACAGCCTGC TGTACGAGTA CTTCACCGTG TACAACGAGC TGACCAAAGT
 4381 GAAATACGTG ACCGAGGGAA TGAGAAAGCC CGCCTTCCTG AGCGGCGAGC AGAAAAAAGC
 4441 CATCGTGGAC CTGCTGTTCA AGACCAACCG GAAAGTGACC GTGAAGCAGC TGAAAGAGGA
 4501 CTACTTCAAG AAAATCGAGT GCTTCGACTC CGTGGAAATC TCCGGCGTGG AAGATCGGTT
 4561 CAACGCCTCC CTGGGCACAT ACCACGATCT GCTGAAAATT ATCAAGGACA AGGACTTCCT
 4621 GGACAATGAG GAAAACGAGG ACATTCTGGA AGATATCGTG CTGACCCTGA CACTGTTTGA
 4681 GGACAGAGAG ATGATCGAGG AACGGCTGAA AACCTATGCC CACCTGTTCG ACGACAAAGT
 4741 GATGAAGCAG CTGAAGCGGC GGAGATACAC CGGCTGGGGC AGGCTGAGCC GGAAGCTGAT
 4801 CAACGGCATC CGGGACAAGC AGTCCGGCAA GACAATCCTG GATTTCCTGA AGTCCGACGG
 4861 CTTCGCCAAC AGAAACTTCA TGCAGCTGAT CCACGACGAC AGCCTGACCT TTAAAGAGGA
 4921 CATCCAGAAA GCCCAGGTGT CCGGCCAGGG CGATAGCCTG CACGAGCACA TTGCCAATCT
 4981 GGCCGGCAGC CCCGCCATTA AGAAGGGCAT CCTGCAGACA GTGAAGGTGG TGGACGAGCT
 5041 CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA GAACATCGTG ATCGAAATGG CCAGAGAGAA
 5101 CCAGACCACC CAGAAGGGAC AGAAGAACAG CCGCGAGAGA ATGAAGCGGA TCGAAGAGGG
 5161 CATCAAAGAG CTGGGCAGCC AGATCCTGAA AGAACACCCC GTGGAAAACA CCCAGCTGCA
 5221 GAACGAGAAG CTGTACCTGT ACTACCTGCA GAATGGGCGG GATATGTACG TGGACCAGGA
 5281 ACTGGACATC AACCGGCTGT CCGACTACGA TGTGGACGCT ATCGTGCCTC AGAGCTTTCT
 5341 GAAGGACGAC TCCATCGATA ACAAAGTGCT GACTCGGAGC GACAAGAACC GGGGCAAGAG
 5401 CGACAACGTG CCCTCCGAAG AGGTCGTGAA GAAGATGAAG AACTACTGGC GCCAGCTGCT
 5461 GAATGCCAAG CTGATTACCC AGAGGAAGTT CGACAATCTG ACCAAGGCCG AGAGAGGCGG
 5521 CCTGAGCGAA CTGGATAAGG CCGGCTTCAT CAAGAGACAG CTGGTGGAAA CCCGGCAGAT
 5581 CACAAAGCAC GTGGCACAGA TCCTGGACTC CCGGATGAAC ACTAAGTACG ACGAGAACGA
 5641 CAAACTGATC CGGGAAGTGA AAGTGATCAC CCTGAAGTCC AAGCTGGTGT CCGATTTCCG
 5701 GAAGGATTTC CAGTTTTACA AAGTGCGCGA GATCAACAAC TACCACCACG CCCACGACGC
 5761 CTACCTGAAC GCCGTCGTGG GAACCGCCCT GATCAAAAAG TACCCTAAGC TGGAAAGCGA
 5821 GTTCGTGTAC GGCGACTACA AGGTGTACGA CGTGCGGAAG ATGATCGCCA AGAGCGAGCA
 5881 GGAAATCGGC AAGGCTACCG CCAAGTACTT CTTCTACAGC AACATCATGA ACTTTTTCAA
 5941 GACCGAGATT ACCCTGGCCA ACGGCGAGAT CCGGAAGCGG CCTCTGATCG AGACAAACGG
 6001 CGAAACAGGC GAGATCGTGT GGGATAAGGG CCGGGACTTT GCCACCGTGC GGAAAGTGCT
 6061 GTCTATGCCC CAAGTGAATA TCGTGAAAAA GACCGAGGTG CAGACAGGCG GCTTCAGCAA
 6121 AGAGTCTATC CTGCCCAAGA GGAACAGCGA CAAGCTGATC GCCAGAAAGA AGGACTGGGA
 6181 CCCTAAGAAG TACGGCGGCT TCGACAGCCC CACCGTGGCC TATTCTGTGC TGGTGGTGGC
 6241 CAAAGTGGAA AAGGGCAAGT CCAAGAAACT GAAGAGTGTG AAAGAGCTGC TGGGGATCAC
 6301 CATCATGGAA AGAAGCAGCT TCGAGAAGAA TCCCATCGAC TTTCTGGAAG CCAAGGGCTA
 6361 CAAAGAAGTG AAAAAGGACC TGATCATCAA GCTGCCTAAG TACTCCCTGT TCGAGCTGGA
 6421 AAACGGCCGG AAGAGAATGC TGGCCTCTGC CGGCGAACTG CAGAAGGGAA ACGAACTGGC
 6481 CCTGCCCTCC AAATATGTGA ACTTCCTGTA CCTGGCCAGC CACTATGAGA AGCTGAAGGG
 6541 CTCCCCCGAG GATAATGAGC AGAAACAGCT GTTTGTGGAA CAGCACAAAC ACTACCTGGA
 6601 CGAGATCATC GAGCAGATCA GCGAGTTCTC CAAGAGAGTG ATCCTGGCCG ACGCTAATCT
 6661 GGACAAGGTG CTGAGCGCCT ACAACAAGCA CAGAGACAAG CCTATCAGAG AGCAGGCCGA
 6721 GAATATCATC CACCTGTTTA CCCTGACCAA TCTGGGAGCC CCTGCCGCCT TCAAGTACTT
 6781 TGACACCACC ATCGACCGGA AGAGGTACAC CAGCACCAAA GAGGTGCTGG ACGCCACCCT
 6841 GATCCACCAG AGCATCACCG GCCTGTACGA GACACGGATC GACCTGTCTC AGCTGGGAGG
 6901 CGACGCCTAT CCCTATGACG TGCCCGATTA TGCCAGCCTG GGCAGCGGCT CCCCCAAGAA
 6961 AAAACGCAAG GTGGAAGATC CTAAGAAAAA GCGGAAAGTG GACGGCATTG GTAGTGGGAG
 7021 CAACGGCAGC AGCGGATCCA GCGAGCTGAT TAAGGAGAAC ATGCACATGA AGCTGTACAT
 7081 GGAGGGCACC GTGGACAACC ATCACTTCAA GTGCACATCC GAGGGCGAAG GCAAGCCCTA
 7141 CGAGGGCACC CAGACCATGA GAATCAAGGT GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT
 7201 CGACATCCTG GCTACTAGCT TCCTCTACGG CAGCAAGACC TTCATCAACC ACACCCAGGG
 7261 CATCCCCGAC TTCTTCAAGC AGTCCTTCCC TGAGGGCTTC ACATGGGAGA GAGTCACCAC
 7321 ATACGAAGAC GGGGGCGTGC TGACCGCTAC CCAGGACACC AGCCTCCAGG ACGGCTGCCT
 7381 CATCTACAAC GTCAAGATCA GAGGGGTGAA CTTCACATCC AACGGCCCTG TGATGCAGAA
 7441 GAAAACACTC GGCTGGGAGG CCTTCACCGA GACGCTGTAC CCCGCTGACG GCGGCCTGGA
 7501 AGGCAGAAAC GACATGGCCC TGAAGCTCGT GGGCGGGAGC CATCTGATCG CAAACATCAA
 7561 GACCACATAT AGATCCAAGA AACCCGCTAA GAACCTCAAG ATGCCTGGCG TCTACTATGT
 7621 GGACTACAGA CTGGAAAGAA TCAAGGAGGC CAACAACGAG ACCTACGTCG AGCAGCACGA
 7681 GGTGGCAGTG GCCAGATACT GCGACCTCCC TAGCAAACTG GGGCACAAGC TTAATGGCGG
 7741 TGGCGGAGGG ATGGATGCTA AGTCACTAAC TGCCTGGTCC CGGACACTGG TGACCTTCAA
 7801 GGATGTATTT GTGGACTTCA CCAGGGAGGA GTGGAAGCTG CTGGACACTG CTCAGCAGAT
 7861 CGTGTACAGA AATGTGATGC TGGAGAACTA TAAGAACCTG GTTTCCTTGG GTTATCAGCT
 7921 TACTAAGCCA GATGTGATCC TCCGGTTGGA GAAGGGAGAA GAGCCCTAGT AAggccgcga
 7981 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
 8041 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
 8101 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
 8161 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
 8221 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
 8281 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
 8341 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
 8401 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
 8461 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
 8521 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
 8581 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg
 8641 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
 8701 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
 8761 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
 8821 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
 8881 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
 8941 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
 9001 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
 9061 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
 9121 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
 9181 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
 9241 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
 9301 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
 9361 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
 9421 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
 9481 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
 9541 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
 9601 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
 9661 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
 9721 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
 9781 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
 9841 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
 9901 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
 9961 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
10021 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
10081 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
10141 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
10201 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
10261 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
10321 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
10381 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
10441 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
10501 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
10561 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
10621 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
10681 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
10741 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
10801 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
10861 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
10921 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
10981 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
11041 ctggcttcag cagagcgcag ataccaaata ctgtctttct agtgtagccg tagttaggcc
11101 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
11161 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
11221 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
11281 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
11341 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
11401 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
11461 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
11521 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
11581 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
11641 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
11701 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
11761 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
11821 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
11881 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
11941 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
12001 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
12061 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
12121 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
12181 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
12241 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
12301 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
12361 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
12421 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
12481 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
12541 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
12601 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
12661 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
12721 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
12781 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
12841 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
12901 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
12961 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
13021 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
13081 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
13141 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
13201 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
13261 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
13321 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
13381 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
13441 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
13501 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
13561 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
13621 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
13681 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
13741 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
13801 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
13861 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
13921 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
13981 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
14041 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
14101 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
14161 tagtagt
//

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