| 质粒名称: | PCDNA3-myc-dnmt3a |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 35521 |
| 存储实验室: | Arthur Riggs |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | 8139 bp |
| 载体修饰: | pcDNA3/Myc was constructed by Zhaoxia Chen (see Comments section for details). DNMT1 was cloned into the pcDNA3/Myc vector. |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | DNMT3A |
| 物种来源: | 人源 |
| 插入大小: | 2739 bp |
| GenBank编号 | AF067972 |
| 启动子 | CMV |
| 标签/融合蛋白: | Myc (N terminal on backbone) |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | BamHI(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3/Myc-DNMT3A was a gift from Arthur Riggs (爱迪基因 plasmid # 35521) |
| 参考文献部分: | Physical and functional interactions between the human DNMT3L protein and members of the de novo methyltransferase family. Chen ZX, Mann JR, Hsieh CL, Riggs AD, Chedin F. J Cell Biochem. 2005 Aug 1;95(5):902-17. 10.1002/jcb.20447 PubMed 15861382 |
pcDNA3/Myc-DNMT3A
价格：1000元
联系方式：I47-825O-882O
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质粒基本信息
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LOCUS pcDNA3/Myc-DNMT3A 8166 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Mammalian expression of human DNMT3A with myc tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA3/Myc-DNMT3A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8166)
AUTHORS Chen ZX, Mann JR, Hsieh CL, Riggs AD, Chedin F
TITLE Physical and functional interactions between the human DNMT3L
protein and members of the de novo methyltransferase family.
JOURNAL J Cell Biochem. 2005 Aug 1;95(5):902-17.
PUBMED 15861382
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCS2FLAG|
FEATURES Location/Qualifiers
source 1..8166
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 895..904
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 904..933
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 940..3678
/codon_start=1
/product="DNA methyltransferase 3A"
/label=DNMT3A
/translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFW
LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
promoter complement(3719..3737)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3720..3737)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3757..3774)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3763..3987
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4033..4461
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4120..4139)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4330..4351
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4470..4490)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4475..4804
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4655..4790
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4717..4736
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4871..5665
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4925..4944)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5535..5554
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5839..5960
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5876..5895)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5930..5949
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6009..6025)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6009..6025)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6022..6044)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6033..6049
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6057..6087)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6102..6123
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6240..6257)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6411..6999)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6491..6510)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7170..8030)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7793..7812
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8031..8135)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
901 atggagcaga agctgatctc agaggaggac ctggaattca tgcccgccat gccctccagc
961 ggccccgggg acaccagcag ctctgctgcg gagcgggagg aggaccgaaa ggacggagag
1021 gagcaggagg agccgcgtgg caaggaggag cgccaagagc ccagcaccac ggcacggaag
1081 gtggggcggc ctgggaggaa gcgcaagcac cccccggtgg aaagcggtga cacgccaaag
1141 gaccctgcgg tgatctccaa gtccccatcc atggcccagg actcaggcgc ctcagagcta
1201 ttacccaatg gggacttgga gaagcggagt gagccccagc cagaggaggg gagccctgct
1261 ggggggcaga agggcggggc cccagcagag ggagagggtg cagctgagac cctgcctgaa
1321 gcctcaagag cagtggaaaa tggctgctgc acccccaagg agggccgagg agcccctgca
1381 gaagcgggca aagaacagaa ggagaccaac atcgaatcca tgaaaatgga gggctcccgg
1441 ggccggctgc ggggtggctt gggctgggag tccagcctcc gtcagcggcc catgccgagg
1501 ctcaccttcc aggcggggga cccctactac atcagcaagc gcaagcggga cgagtggctg
1561 gcacgctgga aaagggaggc tgagaagaaa gccaaggtca ttgcaggaat gaatgctgtg
1621 gaagaaaacc aggggcccgg ggagtctcag aaggtggagg aggccagccc tcctgctgtg
1681 cagcagccca ctgaccccgc atcccccact gtggctacca cgcctgagcc cgtggggtcc
1741 gatgctgggg acaagaatgc caccaaagca ggcgatgacg agccagagta cgaggacggc
1801 cggggctttg gcattgggga gctggtgtgg gggaaactgc ggggcttctc ctggtggcca
1861 ggccgcattg tgtcttggtg gatgacgggc cggagccgag cagctgaagg cacccgctgg
1921 gtcatgtggt tcggagacgg caaattctca gtggtgtgtg ttgagaagct gatgccgctg
1981 agctcgtttt gcagtgcgtt ccaccaggcc acgtacaaca agcagcccat gtaccgcaaa
2041 gccatctacg aggtcctgca ggtggccagc agccgcgcgg ggaagctgtt cccggtgtgc
2101 cacgacagcg atgagagtga cactgccaag gccgtggagg tgcagaacaa gcccatgatt
2161 gaatgggccc tggggggctt ccagccttct ggccctaagg gcctggagcc accagaagaa
2221 gagaagaatc cctacaaaga agtgtacacg gacatgtggg tggaacctga ggcagctgcc
2281 tacgcaccac ctccaccagc caaaaagccc cggaagagca cagcggagaa gcccaaggtc
2341 aaggagatta ttgatgagcg cacaagagag cggctggtgt acgaggtgcg gcagaagtgc
2401 cggaacattg aggacatctg catctcctgt gggagcctca atgttaccct ggaacacccc
2461 ctcttcgttg gaggaatgtg ccaaaactgc aagaactgct ttctggagtg tgcgtaccag
2521 tacgacgacg acggctacca gtcctactgc accatctgct gtgggggccg tgaggtgctc
2581 atgtgcggaa acaacaactg ctgcaggtgc ttttgcgtgg agtgtgtgga cctcttggtg
2641 gggccggggg ctgcccaggc agccattaag gaagacccct ggaactgcta catgtgcggg
2701 cacaagggta cctacgggct gctgcggcgg cgagaggact ggccctcccg gctccagatg
2761 ttcttcgcta ataaccacga ccaggaattt gaccctccaa aggtttaccc acctgtccca
2821 gctgagaaga ggaagcccat ccgggtgctg tctctctttg atggaatcgc tacagggctc
2881 ctggtgctga aggacttggg cattcaggtg gaccgctaca ttgcctcgga ggtgtgtgag
2941 gactccatca cggtgggcat ggtgcggcac caggggaaga tcatgtacgt cggggacgtc
3001 cgcagcgtca cacagaagca tatccaggag tggggcccat tcgatctggt gattgggggc
3061 agtccctgca atgacctctc catcgtcaac cctgctcgca agggcctcta cgagggcact
3121 ggccggctct tctttgagtt ctaccgcctc ctgcatgatg cgcggcccaa ggagggagat
3181 gatcgcccct tcttctggct ctttgagaat gtggtggcca tgggcgttag tgacaagagg
3241 gacatctcgc gatttctcga gtccaaccct gtgatgattg atgccaaaga agtgtcagct
3301 gcacacaggg cccgctactt ctggggtaac cttcccggta tgaacaggcc gttggcatcc
3361 actgtgaatg ataagctgga gctgcaggag tgtctggagc atggcaggat agccaagttc
3421 agcaaagtga ggaccattac tacgaggtca aactccataa agcagggcaa agaccagcat
3481 tttcctgtct tcatgaatga gaaagaggac atcttatggt gcactgaaat ggaaagggta
3541 tttggtttcc cagtccacta tactgacgtc tccaacatga gccgcttggc gaggcagaga
3601 ctgctgggcc ggtcatggag cgtgccagtc atccgccacc tcttcgctcc gctgaaggag
3661 tattttgcgt gtgtgtaagg atccgcggcc gctcgagcat gcatctagag ggccctattc
3721 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
3781 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
3841 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
3901 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
3961 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
4021 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
4081 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
4141 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
4201 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
4261 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
4321 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
4381 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
4441 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
4501 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
4561 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
4621 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
4681 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
4741 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
4801 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
4861 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
4921 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
4981 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
5041 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
5101 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
5161 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
5221 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
5281 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
5341 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
5401 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
5461 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
5521 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
5581 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
5641 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
5701 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
5761 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
5821 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
5881 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
5941 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
6001 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
6061 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
6121 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
6181 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
6241 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
6301 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
6361 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
6421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
6481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
6541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
6601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
6661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
6721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
6781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
6841 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
6901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
6961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
7021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
7081 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
7141 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
7201 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
7261 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
7321 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
7381 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
7441 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
7501 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
7561 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
7621 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
7681 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
7741 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
7801 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
7861 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
7921 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
7981 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
8041 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
8101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
8161 gacgtc
//
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