| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MED23-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001589 |
| 产品用途: | 3' AAVLINK plasmid for MED23 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MED23 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MED23-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MED23-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MED23-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MED23-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MED23-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MED23-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MED23-FLAG 6728 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6728)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6728
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3441
/codon_start=1
/label=MED23
/translation="AHYRHSSNKIMCLRKAVLI*KKMWRRSIGSGSQ*ATKTTLLPTSL
CRAPLLSFFVFSGKCSWKQIILIRLAIEY*RELEPGPW*PM*GHLQISWYMSFLHQQGV
SNSINALKFLMTWYGSITLLHWTD*FSAWPCVVTKEMKPRFVIS*FSCCYSNQTILEIE
*VTL*RKIPQSTGYRMTGTPST*IITRNIQRSCILRASRNRWILLYRSSLPICPSILGM
CVFDSFQYLI**STDF*SCFRYPNHWRLYWII*EAYINFMIVQ*LICITLCTIMKCT*E
TAHFSNENSSMRSLAL*RIIDRRAGV*VTLT*NAL*MHERKILGFQMTPTIAD*LAD*S
IRWLANLLVPFQTVTGDSMSFPTQLPMLSMLLVWSSWPWQFQAKKLGMPF*MLS*KVSL
*CQERTLQHG*MQLV*SSLPYQSHIGLFFMIEL*VSSAAPA*RLKQSGLAIHSASLISL
PVISPTLR*VVAIR*LLHMLCGTILASDNFLSFQSFLLKYFFL**RPNSSCFMYTILLD
HFYKDFSKRELVV**RLVWRFMTCC*MLTSVAPI*ITWIPSVTSSIT*SICLLVTA*KS
K*RRLSVT*NQL*NFVFDSSHTLARWSQLQCLHKP*TVGLQHLSLIRCPCLYQ*L"
misc_feature 1619..1624
/label=SDS
misc_feature 1693..1698
/label=SDS
misc_feature 2164..2169
/label=SDS
misc_feature 2310..2315
/label=SDS
CDS 3448..3513
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3517..3948
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3661..3666
/label=SDS
repeat_region 3991..4131
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3991..4120
/label=AAV2 ITR
rep_origin 4206..4661
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4943..5047
/gene="bla"
/label=AmpR promoter
CDS 5048..5908
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6079..6667
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggccc actacaggca
1561 ttcttcaaac aaaataatgt gcctcaggaa agccgtttta atctgaaaaa aaatgtggag
1621 gaggagtata ggaagtggaa gtcaatgagc aacgaaaacg acattattac ccacttctct
1681 atgcagggct cccctcctct ctttctttgt cttctctgga aaatgctctt ggaaacagat
1741 catattaatc agattggcta tagagtatta gagagaattg gagccagggc cttggtagcc
1801 catgtgagga catttgcaga tttcctggta tatgagtttt ctacatcagc agggggtcag
1861 caactcaata aatgcattga aattcttaat gacatggtat ggaagtataa cattgttaca
1921 ctggacagat taattctctg cctggccatg cgtagtcacg aaggaaatga agcccaggtt
1981 tgttatttca taattcagtt gctgttactc aaaccaaacg attttagaaa tcgagtaagt
2041 gactttgtga aggaaaattc cccagagcac tggttacaga atgactggca caccaagcac
2101 atgaattatc acaagaaata tccagagaag ttgtattttg agggcctcgc ggaacaggtg
2161 gatcctcctg tacagatcca gtctccctat ctgcccatct attttgggaa tgtgtgtctt
2221 cgattccttc cagtatttga tatagtaatc cacagatttt tagagttgct tccggtatcc
2281 aaatcactgg agactctact ggatcatcta ggaggcttat ataaatttca tgatcgtcca
2341 gtgacttatc tgtataacac tctgcactat tatgaaatgc acctgagaga ccgcgcattt
2401 ctcaaacgaa aactcgtcca tgcgatcatt ggctctctga aggataatcg accgcagggc
2461 tggtgtctaa gtgacactta cctgaaatgc gctatgaatg cacgagagga aaatccttgg
2521 gttccagatg acacctacta ttgcagattg attggcagac tagtcgatac gatggctggc
2581 aaatctcctg gtccctttcc aaactgtgac tggagattca atgagtttcc caacccagct
2641 gcccatgctc tccatgttac ttgtgtggag ctcatggcct tggcagtttc aggcaaagaa
2701 gttgggaatg cccttctaaa tgttgtccta aaaagtcagc ctttagtgcc aagagagaac
2761 attacagcat ggatgaatgc aattggtttg atcatcactg ccctaccaga gccatattgg
2821 attgttcttc atgatcgaat tgtgagtgtc atcagcagcc ccagcttgac gtctgaaaca
2881 gagtgggttg gctatccatt ccgcctcttt gatttcactg cctgtcatca gtcctactct
2941 gagatgagtt gtagctatac gttagctctt gcacatgctg tgtggcacca ttctagcatc
3001 ggacaacttt ctctcattcc aaagtttctt actgaagtac ttcttcctat agtgaagacc
3061 gaattccagt tgctttatgt ataccatctt gttggaccat ttttacaaag atttcagcaa
3121 gagagaactc gttgtatgat agagattggt gtggcgtttt atgacatgct gctgaatgtt
3181 gaccagtgta gcacccattt aaattacatg gatcccatct gtgacttcct ctatcacatg
3241 aagtatatgt ttactggtga cagcgtgaaa gagcaagtag agaagattat ctgtaactta
3301 aaaccagctt taaaacttcg tcttcgattc atcacacaca ttagcaagat ggagccagct
3361 gcagtgcctc cacaagccat gaacagtggg tctccagcac ctcagtctaa tcaggtgccc
3421 gtgtctttac cagtaactca gtgtacagac tacaaagacc atgacggtga ttataaagat
3481 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
3541 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
3601 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
3661 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
3721 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
3781 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
3841 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
3901 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac
3961 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
4021 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
4081 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
4141 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
4201 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4261 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
4321 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4381 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
4441 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4501 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
4561 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
4621 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
4681 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
4741 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
4801 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
4861 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
4921 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
4981 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
5041 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
5101 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
5161 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
5221 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5281 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5341 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
5401 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
5461 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5521 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
5581 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
5641 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
5701 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
5761 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
5821 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
5881 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
5941 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
6001 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
6061 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
6121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
6181 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6421 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6481 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6541 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
6601 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
6661 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
6721 tcacatgt
//
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