pAAVLINKv1-pCALM1-Cre-3'-CDK5RAP2-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-CDK5RAP2-FLAG          6695 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3408
                     /codon_start=1
                     /label=CDK5RAP2
                     /translation="GALPSTAAD*GWPWHLCHLPSTHEHHD*GI*GVAAGQ*CGLLCGR
                     GFPGTAESMC*AAGEIGKAISQRKISWSGNEHPE*TDGED*GRQLNLPTSSA*IS*AFS
                     LSCAL*L*NI*KVLLLTRPEAR**DREDFSYGEQFFSRLTNGTHTGNSNFEKAFRRIY*
                     NK*EATETVGTARI*ICSRFYKHFCFWFRAS*FSNIRNSFLEEAEPGPQCNAH*RIQR*
                     TEGE*QITRVPLQEDREPGAPSAGVCQREGRK*KAAERRQREGETQPAADPGGPLQRPG
                     AEQGAGGGEVEAAAALTE*QAIAVSPSGAEGV*EAG*RAQETERGVGRRLEGAGSFQGP
                     AQPPDGDPGSALATRKEHRNQQHSAEQAQGTAGKGGREGTGRSPHSGCPSRVHP*GAPS
                     A*QTRW*QISHGK**FI*SV*FLPGSDTKISFRDSSTLWE*HGLPLLRQWQFGN*HSVC
                     VPPGHWPPPVGQQEWPPCPGPD*GL*GPAQTDQPGTEAPC*NGHSNPRGSQLHKSRAGN
                     KGSTPSTTEQVCEQCEHGQADPGRGLQAAEASLESLTPRGWPVPPSL*ADWRNEGRGHQ
                     TT*KIV*TRKEVAKHHEAFAAEQAPGKSHL*SIGRNPQNPSEGQRKPGA*AWGSPSRNM
                     QSQQTRL"
     misc_feature    2300..2305
                     /label=SDS
     misc_feature    2339..2344
                     /label=SDS
     misc_feature    2342..2347
                     /label=SDS
     misc_feature    2508..2513
                     /label=SDS
     CDS             3415..3480
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3484..3915
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3628..3633
                     /label=SDS
     repeat_region   3958..4098
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3958..4087
                     /label=AAV2 ITR
     rep_origin      4173..4628
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4910..5014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5015..5875
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6046..6634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagctctc ccttcaacgg
     1501 cagcagatta aggatggcca tggcatctgt gtcatctccc gtcaacacat gaacaccatg
     1561 attaaggcat ttgaggagtt gctgcaggcc agtgatgtgg attactgtgt ggccgagggt
     1621 ttccaggaac agctgaatca atgtgctgag ctgctggaga aattggaaaa gctatttctc
     1681 aacggaaaat cagttggagt ggaaatgaac acccagaatg aactgatgga gaggattgag
     1741 gaagacaact taacctacca acatcttctg cctgaatctc ctgagccttc agcctctcat
     1801 gcgctctctg attatgaaac atctgaaaag tccttcttct cacgagacca gaagcaagat
     1861 aatgagacag agaagacttc agttatggtg aacagttttt ctcaagactt actaatggaa
     1921 cacatacagg aaattcgaac tttgagaaag cgtttagaag aatctattaa aacaaatgag
     1981 aagctacgga aacagttgga acggcaagga tctgaatttg ttcaaggttc tacaagcatt
     2041 tttgcttctg gttcagagct tcatagttct ctaacatcag aaattcattt cttgaggaag
     2101 cagaaccagg ccctcaatgc aatgctcatt aaaggatcca gagataaaca gaaggagaat
     2161 gacaaattac gagagtccct ctccaggaag accgtgagcc tggagcacct tcagcgggag
     2221 tatgccagcg tgaaggaaga aaatgaaagg ctgcagaaag aaggcagcga gaaggagaga
     2281 cacaaccagc agctgatcca ggaggtccgc tgcagcggcc aggagctgag cagggtgcag
     2341 gaggaggtga agttgaggca gcagctgctc tcacagaatg acaagctatt gcagtctctc
     2401 cgagtggagc tgaaggcgta tgagaagctg gatgaagagc acaggagact gagagaggcg
     2461 tcgggagaag gctggaaggg gcaggatcct ttcagggacc tgcacagcct cctgatggag
     2521 atccaggctc tgcgcttgca actagaaagg agcatcgaaa ccagcagcac tctgcagagc
     2581 aggctcaagg aacagctggc aaggggggca gagaaggcac aggaaggagc cctcactctg
     2641 gctgtccaag ccgtgtccat ccctgaggtg ccccttcagc ctgacaaaca cgatggtgac
     2701 aaatatccca tggaaagtga taattcattt gatctgtttg attcctccca ggcagtgaca
     2761 ccaaaatcag tttcagagac tcctccactc tctgggaatg acacggactc cctctcctgc
     2821 gacagtggca gttcggcaac tagcactccg tgtgtgtccc gcctggtcac tggccaccac
     2881 ctgtgggcca gcaagaatgg ccgccatgtc ctgggcctga ttgaggacta tgaggccctg
     2941 ctcaaacaga tcagccaggg acagaggctc cttgctgaaa tggacattca aacccaagag
     3001 gctcccagct ccacaagtca agagctggga acaaagggtc cacacccagc accactgagc
     3061 aagtttgtga gcagtgtgag cacggccaag ctgaccctgg aagaggccta caggcggctg
     3121 aagcttctct ggagagtctc actccccgag gatggccagt gcccccttca ctgtgagcag
     3181 attggagaaa tgaaggcaga ggtcaccaaa ctacataaaa aattgtttga acaagaaaag
     3241 aagttgcaaa acaccatgaa gcttttgcag ctgagcaagc gccaggaaaa agtcatcttt
     3301 gatcaattgg tcgtaaccca caaaatcctt cggaaggcca gaggaaacct ggagcttagg
     3361 cctgggggag cccatccagg aacatgcagt cccagcagac caggctcctg tacagactac
     3421 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     3481 taactcgaga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3541 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3601 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca
     3661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3721 ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3781 tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3841 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     3901 tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
     3961 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4021 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4081 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4141 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4381 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4501 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4621 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4681 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4741 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4801 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4861 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4921 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4981 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5041 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5101 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5161 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5221 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5281 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5341 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5401 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5461 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5521 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5581 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5641 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5701 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5761 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5821 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5881 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6661 gttcctggcc ttttgctggc cttttgctca catgt
//