| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001400 |
| 产品用途: | 3' AAVLINK plasmid for NRXN2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NRXN2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN2-FLAG 7053 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7053)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7053
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3766
/codon_start=1
/label=NRXN2
/translation="GYIHYVFDLGNGPSLMKGNSDKPVNDNQWHNVVVSRDPGNVHTLK
IDSRTVTQHSNGARNLDLKGELYIGGLSKNMFSNLPKLVASRDGFQGCLASVDLNGRLP
DLIADALHRIGQVERGCDGPSTTCTEESCANQGVCLQQWDGFTCDCTMTSYGGPVCNDP
GTTYIFGKGGALITYTWPPNDRPSTRMDRLAVGFSTHQRSAVLVRVDSASGLGDYLQLH
IDQGTVGVIFNVGTDDITIDEPNAIVSDGKYHVVRFTRSGGNATLQVDSWPVNERYPAG
NFDNERLAIARQRIPYRLGRVVDEWLLDKGRQLTIFNSQAAIKIGGRDQGRPFQGQVSG
LYYNGLKVLALAAESDPNVRTEGHLRLVGEGPSVLLSAETTATTLLADMATTIMETTTT
MATTTTRRGRSPTLRDSTTQNTDDLLVASAECPSDDEDLEECEPSTGGELILPIITEDS
LDPPPVATRSPFVPPPPTFYPFLTGVGATQDTLPPPAARRPPSGGPCQAERDDSDCEEP
IEASGFASGEVFDSSLPPTDDEDFYTTFPLVTDRTTLLSPRKPAPRPNLRTDGATGAPG
VLFAPSAPAPNLPAGKMNHRDPLQPLLENPPLGPGAPTSFEPRRPPPLRPGVTSAPGFP
HLPTANPTGPGERGPPGAVEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEG
SYQVDQSRNYISNSAQSNGAVVKEKAPAAPKTPSKAKKNKDKEYYV"
misc_feature 3205..3210
/label=SDS
misc_feature 3586..3591
/label=SDS
CDS 3773..3838
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3842..4273
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3986..3991
/label=SDS
repeat_region 4316..4456
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4316..4445
/label=AAV2 ITR
rep_origin 4531..4986
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5268..5372
/gene="bla"
/label=AmpR promoter
CDS 5373..6233
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6404..6992
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggt acatccacta
1561 cgtgtttgac ctggggaatg gcccgtcctt gatgaagggg aactcagaca aaccagtcaa
1621 tgacaaccag tggcacaacg tggtggtgtc cagggaccca ggcaacgtgc acacgctcaa
1681 gattgactcc cgcactgtca cgcagcactc caatggcgcc cgaaacctcg atctcaaagg
1741 ggagttgtac attggcggtc tgagcaagaa tatgttcagc aacctgccca agctggtggc
1801 ctcccgggat ggctttcagg gctgcctggc ctcagtggac ctcaacggac gtctcccaga
1861 cctcatcgcc gacgccctgc accgcattgg gcaggtggag aggggctgtg atggccccag
1921 caccacctgc actgaagagt cctgtgccaa ccagggcgtc tgcttgcagc agtgggatgg
1981 cttcacctgc gactgcacca tgacttccta tggaggccct gtctgcaatg atcccgggac
2041 cacatacatc tttgggaagg ggggagcgct catcacctac acgtggcccc ccaatgacag
2101 gcccagcacg aggatggatc gcctggccgt gggcttcagc acccaccagc ggagcgctgt
2161 gctggtgcgg gtggacagcg cctccggcct tggagactac ctgcagctgc acatcgacca
2221 gggcaccgtg ggggtgatct ttaacgtggg cacggacgac attaccatcg acgagcccaa
2281 cgccatagta agcgacggca aataccacgt ggtgcgcttc actcgaagcg gcggcaacgc
2341 caccctgcag gtggacagct ggccggtcaa cgagcggtac ccggcaggaa actttgataa
2401 cgagcgcctg gcgattgcta gacagagaat cccctaccgg cttggtcgag tagtagatga
2461 gtggctgctc gacaaaggcc gccagctgac catcttcaac agccaggctg ccatcaagat
2521 cgggggccgg gatcagggcc gccccttcca gggccaggtg tccggcctct actacaatgg
2581 gctcaaggtg ctggcgctgg ccgccgagag cgaccccaat gtgcggactg agggtcacct
2641 gcgcctggtg ggggaggggc cgtccgtgct gctcagtgcg gagaccacgg ccaccaccct
2701 gctggctgac atggccacca ccatcatgga gactaccacc accatggcca ctaccaccac
2761 gcgccggggc cgctccccca cactgaggga cagcaccacc cagaacacag atgacctgct
2821 ggtggcctct gctgagtgtc caagcgatga tgaggacctg gaggagtgtg agcccagtac
2881 tggaggagag ttaatattgc ccattatcac ggaggactcc ttagaccccc ctcccgtggc
2941 cacccgatcc cccttcgtgc ccccgccccc taccttctac cccttcctca cgggagtggg
3001 cgccacccaa gacacgctgc ccccgcccgc cgcgcgccgc ccgccctctg ggggcccgtg
3061 ccaggccgag cgggacgaca gcgactgcga ggagcccatc gaggcctcgg gcttcgcctc
3121 cggggaggtc tttgactcca gcctcccccc cacggacgac gaggactttt acaccacctt
3181 tcccctggtc acggaccgca ccaccctcct gtcaccccgc aaacccgctc cccggcccaa
3241 cctcaggaca gatggggcca cgggcgcccc tggggtgctg tttgccccct ccgccccggc
3301 ccccaacctg ccggcgggca aaatgaacca ccgagacccg cttcagccct tgctggagaa
3361 cccgcccttg gggcccgggg cccccacgtc ctttgagccg cggaggcccc ctcccctgcg
3421 ccccggcgtg acctcagccc ccggcttccc ccatctgccc acagccaacc ccacagggcc
3481 tggggagcgg ggcccgccgg gcgcagtgga ggtgatccgg gagtccagca gcaccacggg
3541 catggtggtg ggcattgtgg cggcggcggc gctctgcatc ctcatcctcc tctacgccat
3601 gtataagtac cgcaatcgtg atgagggctc ctaccaggtg gaccagagcc gaaactacat
3661 cagtaactcg gcccagagca atggggcggt ggtgaaagag aaggccccgg ctgcccccaa
3721 gacgcccagc aaggccaaga agaacaaaga caaggagtat tatgtctgta cagactacaa
3781 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
3841 actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
3901 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
3961 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
4021 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
4081 gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
4141 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
4201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
4261 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
4321 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4381 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4441 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4501 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
4621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4741 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
4861 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
4921 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
4981 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
5041 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
5101 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
5161 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
5221 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5281 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5341 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5401 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5461 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5521 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
5581 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
5641 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
5701 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
5761 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
5821 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
5881 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
5941 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
6001 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
6061 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
6121 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
6181 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6241 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
6301 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
6361 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6421 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6481 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6541 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6601 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
6781 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
6841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
6901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
6961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
7021 tcctggcctt ttgctggcct tttgctcaca tgt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN2-FLAG质粒载体应用举例
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