pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DOCK1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DOCK1-FLAG                6787 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3488
                     /codon_start=1
                     /label=DOCK1
                     /translation="VFVQAL*PAQGV**LHRSGLHLASPCKAS*VVGGCVCGPPHPAGR
                     VPGHHAGTAEGAALPGNHPLLRQRQDVGGGHCLGQGASRAV*ERNV*L*ATQRIAEKTG
                     SVL*KHRQSDQAQA*LFCCWLLRTRVPHIPAGKSFHLPRERV*APGRF*GSALNSVSKR
                     RENEDNISTRRRY*KLSWPVYSVLHSEAQTRSAS*VSQASVRADCKFLQGERGPAI*IF
                     SANPEGREKPRQ*ICEYVDRENHIYNCI*ITWNFKVV*GQVCFHGGNQPPGECHRDHAA
                     DERQDQQHGAAAPG*PQPAHQPALHAPERHRGPSCHGGLRKLRKGLLYRPVPAGAP*GP
                     *KDREAQGPDCLADSFSGRRDQNPWRQSHGGTEAVPREDGGLFQTAEGKGGERVRRPNH
                     ALKSG**KRQPPPVHGAVLHDAFLIPPSVCGLCLFPLIGQHPFQTRLRRVCPGASPAKE
                     NALQVPGQAGQG*PGEGEEGQEEGKKEQQTSRDI*ERI*THRHFPAAV*GCDPFGNDKS
                     PAAPETEEPGDERHWKRKALLGVPLVTVLPANTPSSYTKGQAQLQHAVELGAERHDGDG
                     RGRCPTPSASQRQRGRLREFDGKPGLAGLANTSTSPSTPEASATSTAQQNSASSPSKDN
                     SQADIGGLWDRA"
     misc_feature    1765..1770
                     /label=SDS
     misc_feature    2133..2138
                     /label=SDS
     misc_feature    3429..3434
                     /label=SDS
     CDS             3495..3560
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3576..4007
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3720..3725
                     /label=SDS
     repeat_region   4050..4190
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4050..4179
                     /label=AAV2 ITR
     rep_origin      4265..4720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5002..5106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5107..5967
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6138..6726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtat ttgtacaagc
     1561 tctgtgacct gcacaaggag tgtgataact acaccgaagc ggcttacacc ttgcttctcc
     1621 atgcaaagct tcttaagtgg tcggaggatg tgtgtgtggc ccacctcacc cagcgggacg
     1681 ggtaccaggc caccacgcag ggacagctga aggagcagct ctaccaggaa atcatccact
     1741 acttcgacaa aggcaagatg tgggaggagg ccattgcctt gggcaaggag ctagccgagc
     1801 agtatgagaa cgaaatgttt gattatgagc aactcagcga attgctgaaa aaacaggctc
     1861 agttttatga aaacatcgtc aaagtgatca ggcccaagcc tgactatttt gctgttggct
     1921 actacggaca agggttcccc acattcctgc ggggaaaagt tttcatttac cgagggaaag
     1981 agtatgagcg ccgggaagat tttgaggctc ggctcttaac tcagtttcca aacgccgaga
     2041 aaatgaagac aacatctcca ccaggcgacg atattaaaaa ctctcctggc cagtatattc
     2101 agtgcttcac agtgaagccc aaactcgatc tgcctcctaa gtttcacagg ccagtgtcag
     2161 agcagattgt aagtttttac agggtgaacg aggtccagcg atttgaatat tctcggccaa
     2221 tccggaaggg agagaaaaac ccagacaatg aatttgcgaa tatgtggatc gagagaacca
     2281 tatatacaac tgcatataaa ttacctggaa ttttaaggtg gtttgaggtc aagtctgttt
     2341 tcatggtgga aatcagcccc ctggagaatg ccatcgagac catgcagctg acgaacgaca
     2401 agatcaacag catggtgcag cagcacctgg atgaccccag cctgcccatc aacccgctct
     2461 ccatgctcct gaacggcatc gtggacccag ctgtcatggg gggcttcgca aactacgaaa
     2521 aggccttctt tacagaccgg tacctgcagg agcaccctga ggcccatgaa aagatcgaga
     2581 agctcaagga cctgattgct tggcagattc cttttctggc cgaagggatc agaatccatg
     2641 gagacaaagt cacggaggca ctgaggccgt tccacgagag gatggaggcc tgtttcaaac
     2701 agctgaagga aaaggtggag aaagagtacg gcgtccgaat catgccctca agtctggatg
     2761 atagaagagg cagccgcccc cggtccatgg tgcggtcctt cacgatgcct tcctcatccc
     2821 gccctctgtc tgtggcctct gtctcttccc tctcatcgga cagcacccct tccagaccag
     2881 gctccgacgg gtttgccctg gagcctctcc tgccaaagaa aatgcactcc aggtcccagg
     2941 acaagctgga caaggatgac ctggagaagg agaagaagga caagaagaag gaaaaaagga
     3001 acagcaaaca tcaagagata tttgagaaag aatttaaacc caccgacatt tccctgcagc
     3061 agtctgaggc tgtgatcctt tcggaaacga taagtcccct gcggccccag agaccgaaga
     3121 gccaggtgat gaacgtcatt ggaagcgaaa ggcgcttctc ggtgtccccc tcgtcaccgt
     3181 cctcccagca aacaccccct ccagttacac caagggccaa gctcagcttc agcatgcagt
     3241 cgagcttgga gctgaacggc atgacgggga cggacgtggc cgatgtccca ccccctctgc
     3301 ctctcaaagg cagcgtggca gattacggga atttgatgga aaaccaggac ttgctgggct
     3361 cgccaacacc tccacctccc cctccacacc agaggcatct gccacctcca ctgcccagca
     3421 aaactccgcc tcctccccct ccaaagacaa ctcgcaagca gacatcggtg gactctggga
     3481 tcgtgcagtg tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact
     3541 acaaggatga cgatgacaag taagaattct gatgactcga gataatcaac ctctggatta
     3601 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3661 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3721 ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc
     3781 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga
     3841 aatttgtgat gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg
     3901 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3961 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc
     4021 ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     4081 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4141 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4201 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4261 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4321 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     4381 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4441 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4501 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4561 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     4621 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     4681 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4741 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4801 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4861 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4921 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4981 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5041 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5101 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5161 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5221 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5281 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5341 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5401 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5461 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5521 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5581 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5641 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5701 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5761 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5821 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5881 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5941 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6001 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6061 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6301 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6361 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6421 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6481 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6541 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6601 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6661 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6721 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6781 cacatgt
//