| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-IQSEC2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001357 |
| 产品用途: | 3' AAVLINK plasmid for IQSEC2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | IQSEC2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-IQSEC2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-IQSEC2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-IQSEC2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-IQSEC2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-IQSEC2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-IQSEC2-FLAG 7067 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7067)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7067
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3780
/codon_start=1
/label=IQSEC2
/translation="GASGYRPVQADLPAGDKA*LGLASFQQ*CGPEAALPNRPQPLQQE
AREGYPVSDRAGLPVRHTGGSGSLHPGAERPQPADDRGIPREPAEAVQQRRVGLCGG*D
GLLLHGSG*CAPEVPVPYPGSG*GPESGATHRSLQPAVLCL*PSPRAPVPEPRHHLHPC
FCHHPPQYRHVQSQRQS*TKDETR*LHQEPERG*QW*RHPPRPPGGHLPAHPGA*TADQ
R*PCVPGAGCGAHDCWKETSPVSPSPSTGLLLPALRGARSKPPPEARVASAGGLPLQ*S
PCGHQNFPEEEDLGDVQFPSVFPPRGNAHAALPEFILPVWDQVAVCSTWWGAKSPHHLQ
CPQPPGPAALYIRPARVHCGGAGDGEIPCGVGAGEAERYDAA*RLTAWRGQGLSEWDDG
PQ*PGGHLRGRRWAQTGRTQQFPARPL*CREAGAA*QRGIAGQHHRRVCY*QSTPSPED
ATSAPTPSARGVQEPEARLQLLILPGLPIWKQAGQGALPDATTANRPGLCLLFICFFHA
PPPPPPPSWP*PRWPGGAA*WAVQAPGPACPVLPRTGPCPATLPPTPAALSSPTSPAAP
TLAPAGLHSTASRLSPTCGATSPLPRPWPSPRAPTLYLGPARQGTQTGGWRTPSVCSTW
PPPPAPAHIPTAPVQSCPPAPSSPQTGP*ALHLQPPPTDDASSRRGWGPWIPATRGLLL
PPPPPPVTIVTTLTHPTPPLLSTPPPTLPSHPALTPSTHLPPWPAARLWAPWHSQPPQC
KPQGQAKPDQHRG"
misc_feature 1983..1988
/label=SDS
misc_feature 2097..2102
/label=SDS
misc_feature 3005..3010
/label=SDS
CDS 3031..3057
/codon_start=1
/product="9xHis affinity tag"
/label=9xHis
/translation="HHHHHHHHH"
misc_feature 3620..3625
/label=SDS
CDS 3787..3852
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3856..4287
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4000..4005
/label=SDS
repeat_region 4330..4470
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4330..4459
/label=AAV2 ITR
rep_origin 4545..5000
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5282..5386
/gene="bla"
/label=AmpR promoter
CDS 5387..6247
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6418..7006
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagcctcc ggctaccggc
1501 ctgtgcaagc agacttacca gcgggagaca aggcatagct gggactcgcc agctttcaac
1561 aatgatgtgg tccagaggcg gcactaccga atcggcctca acctcttcaa caagaagcca
1621 gagaagggta tccagtatct gatcgagcgg ggcttcctgt cagacacacc ggtgggagtg
1681 gctcacttca tcctggagcg gaaaggcctc agccggcaga tgatagggga attcctaggg
1741 aaccggcaga agcagttcaa cagagacgtg ttggactgtg tggtggatga gatggacttc
1801 tcctccatgg atctggatga tgcgctccgg aagttccagt cccatatccg ggttcagggt
1861 gaggcccaga aagtggagcg actcatcgaa gccttcagcc agcggtactg tgtctgtaac
1921 ccagccctcg tgcgccagtt ccggaaccca gacaccatct tcatccttgc ttttgccatc
1981 atcctcctca ataccgacat gtacagtccc agcgtcaaag ctgaacgaaa gatgaaacta
2041 gatgacttca tcaagaacct gagaggggtt gacaatggtg aagacatccc ccgagacctc
2101 ctggtgggca tctaccagcg catccagggg cgtgaactgc ggaccaacga tgaccatgtg
2161 tcccaggtgc aggctgtgga gcgcatgatt gttggaaaga aaccagtcct gtctctccct
2221 caccgtcgac tggtttgctg ctgccagctc tacgaggtgc cagatccaaa ccgcccccag
2281 aggctagggt tgcatcagcg ggaggtcttc ctcttcaatg atctccttgt ggtcaccaaa
2341 attttccaga agaagaagat cttggtgacg tacagtttcc gtcagtcttt ccccctcgtg
2401 gaaatgcaca tgcagctctt ccagaattca tattaccagt ttgggatcaa gttgctgtct
2461 gcagtacctg gtggggagcg aaaagtcctc atcatcttca atgcccccag cctccaggac
2521 cggctgcgct ttacatccga cctgcgcgag tccattgcgg aggtgcagga gatggagaaa
2581 taccgtgtgg agtcggagct ggagaagcag aaaggtatga tgcggcctaa cgcctcacag
2641 cctggagggg ccaaggactc agtgaatggg acgatggccc gcagtagcct ggaggacact
2701 tacggggcag gcgatgggct caaacggggc gcactcagca gttccctgcg agacctctct
2761 gatgcaggga agcgggggcg gcgtaacagc gtgggatcgc tggacagcac catcgaaggg
2821 tctgttatta gcagtccacg ccctcaccag aggatgccac ctccgccccc accccctccg
2881 ccagaggagt acaagagcca gaggcccgtc tccaactcct catccttcct gggctcccta
2941 tttggaagca agcggggcaa ggggcccttc cagatgccac caccgccaac aggccaggcc
3001 tctgcctcct cttcatctgc ttcttccacg caccaccacc accaccacca ccatcatggc
3061 catagccacg gtggcctggg ggtgctgcct gatgggcagt ccaagctcca ggccctgcat
3121 gcccagtatt gccaaggacc gggccctgcc ccgccaccct acctcccacc ccagcagccc
3181 tctcttcccc cacctcccca gcagccccca cccttgcccc agctgggctc cattccaccg
3241 cctcccgcct cagccccacc tgtggggcca catcgccact tccacgccca tggcccagtc
3301 ccagggcccc aacactatac cttgggccgg ccaggcaggg cacccagacg gggggctgga
3361 ggacaccctc agtttgctcc acatggccgc caccccctgc accagcccac atccccactg
3421 cccctgtaca gtcctgcccc ccagcaccct ccagcccaca aacagggccc taagcacttc
3481 atcttcagcc accacccaca gatgatgcca gcagcaggcg cggctggggg ccctggatcc
3541 cggccaccag ggggctccta ctcccacccc caccaccccc agtcaccatt gtcaccacac
3601 tcacccatcc caccccaccc ctcctatcca cccctccccc caccctcccc tcacaccccg
3661 cactcacccc ttccacccac ctccccccat ggcccgctgc acgcctctgg gccccctggc
3721 acagccaacc cccccagtgc aaaccccaag gccaagccaa gccggatcag caccgtggtc
3781 tgtacagact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
3841 gacgatgaca agtaactcga gataatcaac ctctggatta caaaatttgt gaaagattga
3901 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
3961 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
4021 tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
4081 ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt
4141 tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
4201 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
4261 cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac gtgcggaccg
4321 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
4381 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
4441 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
4501 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4561 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4621 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4681 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4741 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4801 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
4861 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
4921 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
4981 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
5041 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
5101 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
5161 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
5221 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
5281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
5341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
5401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
5461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
5521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
5821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
5881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
5941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
6001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
6061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
6121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
6181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
6241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
6301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
6361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
6421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
6481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
6841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
6901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
7021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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