| 质粒名称: | pAAVLINK-EF1a-HA-5'-TNS2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001620 |
| 产品用途: | 5' AAVLINK plasmid for TNS2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TNS2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-TNS2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-TNS2载体质粒基本信息
pAAVLINK-EF1a-HA-5'-TNS2质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-TNS2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-TNS2质粒载体简介
pAAVLINK-EF1a-HA-5'-TNS2质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-TNS2 5754 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5754)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5754
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2894
/codon_start=1
/label=TNS2
/translation="MKSSGPVERLLRALGRRDSSRAASRPRKAEPHSFREKVFRKKPPV
CAVCKVTIDGTGVSCRVCKVATHRKCEAKVTSACQALPPVELRRNTAPVRRIEHLGSTK
SLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAAAFPARPDEQRHRGHLRELAH
VLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSAD
PQHVVVLYCKGNKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVATELQPSQR
RYISYFSGLLSGSIRMNSSPLFLHYVLIPMLPAFEPGTGFQPFLKIYQSMQLVYTSGVY
HIAGPGPQQLCISLEPALLLKGDVMVTCYHKGGRGTDRTLVFRVQFHTCTIHGPQLTFP
KDQLDEAWTDERFPFQASVEFVFSSSPEKIKGSTPRNDPSVSVDYNTTEPAVRWDSYEN
FNQHHEDSVDGSLTHTRGPLDGSPYAQVQRPPRQTPPAPSPEPPPPPMLSVSSDSGHSS
TLTTEPAAESPGRPPPTAAERQELDRLLGGCGVASGGRGAGRETAILDDEEQPTVGGGP
HLGVYPGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAYGGYEGSPQGYAE
ASMEKRRLCRSLSEGLYPYPPEMGKPATGDFGYRAPGYREVVILEDPGLPALYPCPACE
EKLALPTAALYGLRLEREAGE"
misc_feature 794..799
/label=SDS
misc_feature 1819..1824
/label=SDS
misc_feature 1822..1827
/label=SDS
misc_feature 2377..2382
/label=SDS
misc_feature 2383..2388
/label=SDS
misc_feature 2600..2605
/label=SDS
intron 2895..2935
/label=Splicing Donor
misc_feature 2939..2972
/label=lox2272-JT1571
repeat_region 3017..3157
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3017..3146
/label=AAV2 ITR
rep_origin 3232..3687
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3969..4073
/gene="bla"
/label=AmpR promoter
CDS 4074..4934
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5105..5693
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gaagtccagc ggccctgtgg agaggctgct
781 cagagccctg gggaggaggg acagcagccg ggccgcaagc aggcctagga aagctgagcc
841 tcatagcttc cgggagaagg ttttccggaa gaaacctcca gtctgtgcag tatgtaaggt
901 gaccatcgat gggacaggcg tttcgtgcag agtctgcaag gtggcgacgc acagaaaatg
961 tgaagcaaag gtgacttcag cctgtcaggc cttgcctccc gtggagttgc ggcgaaacac
1021 ggccccagtc aggcgcatag agcacctggg atccaccaaa tctctgaacc actcaaagca
1081 gcgcagcact ctgcccagga gcttcagcct ggacccgctc atggagcggc gctgggactt
1141 agacctcacc tacgtgacgg agcgcatctt ggccgccgcc ttccccgcgc ggcccgatga
1201 acagcggcac cggggccacc tgcgcgagct ggcccatgtg ctgcaatcca agcaccggga
1261 caagtacctg ctcttcaacc tttcagagaa aaggcatgac ctgacccgct taaaccccaa
1321 ggttcaagac ttcggctggc ctgagctgca tgctccaccc ctggacaagc tgtgctccat
1381 ctgcaaagcc atggagacat ggctcagtgc tgacccacag cacgtggtcg tactatactg
1441 caagggaaac aagggcaagc ttggggtcat cgtttctgcc tacatgcact acagcaagat
1501 ctctgcaggg gcggaccagg cactggccac tcttaccatg cggaaattct gcgaggacaa
1561 ggtggccaca gaactgcagc cctcccagcg tcgatatatc agctacttca gtgggctgct
1621 atctggctcc atcagaatga acagcagccc tctcttcctg cactatgtgc tcatccccat
1681 gctgccagcc tttgaacctg gcacaggctt ccagcccttc cttaaaatct accagtccat
1741 gcagcttgtc tacacatctg gagtctatca cattgcaggc cctggtcccc agcagctttg
1801 catcagcctg gagccagccc tcctcctcaa aggcgatgtc atggtaacat gttatcacaa
1861 gggtggccgg ggcacagacc ggaccctcgt gttccgagtc cagttccaca cctgcaccat
1921 ccacggacca cagctcactt tccccaagga ccagcttgac gaggcctgga ctgatgagag
1981 gttccccttc caagcctccg tggagtttgt cttctcctcc agccccgaga agatcaaagg
2041 cagcactcca cggaacgacc cctcggtctc tgtcgactac aacaccactg agccagccgt
2101 gcgctgggac tcctatgaga acttcaacca gcaccacgag gacagtgtgg atggctcctt
2161 gacccacacc cggggtcccc tggatggcag tccttatgcc caggtgcagc ggcctccccg
2221 gcagaccccc ccggcaccct ctccagagcc tccaccaccc cccatgctct ctgtcagcag
2281 cgactcaggc cattcctcca cgctgaccac agagccggct gctgagtccc ctggccggcc
2341 gccccctaca gctgctgaac ggcaggagct ggatcgcctc ctaggaggct gcggagtggc
2401 cagtgggggc cggggagctg ggcgcgagac ggccatccta gatgacgaag agcagcccac
2461 tgtgggcgga ggcccccacc tcggagtgta tccaggccat aggcctggcc tcagccgcca
2521 ctgctcctgc cgccagggct accgggagcc ctgcggggtt cccaatgggg gctactaccg
2581 gccagaggga accctggaga ggaggcgact ggcctacggg ggctatgagg gatcccccca
2641 gggctacgcc gaggcctcga tggagaagag gcgcctctgc cgatcgctgt cagaggggct
2701 atacccctac ccacctgaga tggggaaacc agccactggg gactttggct accgcgcccc
2761 aggctaccgg gaggtggtca tcctggagga ccctgggctg cctgccctat acccatgccc
2821 agcctgcgag gagaagctgg cgctgcctac agcagccttg tatggactgc ggctggagag
2881 ggaggctgga gaaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta
2941 ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg
3001 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
3061 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
3121 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
3181 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
3241 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3301 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3361 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3421 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
3481 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
3541 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
3601 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
3661 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
3721 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
3781 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
3841 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
3901 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
3961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
4441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
4501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
4561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
4621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
4681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
4741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
4801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
4861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
4921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
4981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
5401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
5461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
5521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
5581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
5641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
5701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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pAAVLINK-EF1a-HA-5'-TNS2质粒载体应用举例
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