pAAVLINK-EF1a-HA-5'-BRCA1

LOCUS       pAAVLINK-EF1a-HA-5'-BRCA1                7040 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4180
                     /codon_start=1
                     /label=BRCA1
                     /translation="MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCK
                     FCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANS
                     YNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVR
                     TLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAK
                     KAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHAS
                     SLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKV
                     DLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSH
                     DGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIED
                     KIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDF
                     IKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEK
                     ESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPN
                     CTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDS
                     DTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLS
                     GERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPK
                     GLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAL
                     FSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFP
                     VVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSF
                     VKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKGASSSNINEVG
                     SSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPE
                     IKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQ"
     misc_feature    2582..2587
                     /label=SDS
     misc_feature    3589..3594
                     /label=SDS
     intron          4181..4221
                     /label=Splicing Donor
     misc_feature    4225..4258
                     /label=lox2272-JT1571
     repeat_region   4303..4443
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4303..4432
                     /label=AAV2 ITR
     rep_origin      4518..4973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5255..5359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5360..6220
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6391..6979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggatttatct gctcttcgcg ttgaagaagt
      781 acaaaatgtc attaatgcta tgcagaaaat cttagagtgt cccatctgtc tggagttgat
      841 caaggaacct gtctccacaa agtgtgacca catattttgc aaattttgca tgctgaaact
      901 tctcaaccag aagaaagggc cttcacagtg tcctttatgt aagaatgata taaccaaaag
      961 gagcctacaa gaaagtacga gatttagtca acttgttgaa gagctattga aaatcatttg
     1021 tgcttttcag cttgacacag gtttggagta tgcaaacagc tataattttg caaaaaagga
     1081 aaataactct cctgaacatc taaaagatga agtttctatc atccaaagta tgggctacag
     1141 aaaccgtgcc aaaagacttc tacagagtga acccgaaaat ccttccttgc aggaaaccag
     1201 tctcagtgtc caactctcta accttggaac tgtgagaact ctgaggacaa agcagcggat
     1261 acaacctcaa aagacgtctg tctacattga attgggatct gattcttctg aagataccgt
     1321 taataaggca acttattgca gtgtgggaga tcaagaattg ttacaaatca cccctcaagg
     1381 aaccagggat gaaatcagtt tggattctgc aaaaaaggct gcttgtgaat tttctgagac
     1441 ggatgtaaca aatactgaac atcatcaacc cagtaataat gatttgaaca ccactgagaa
     1501 gcgtgcagct gagaggcatc cagaaaagta tcagggtagt tctgtttcaa acttgcatgt
     1561 ggagccatgt ggcacaaata ctcatgccag ctcattacag catgagaaca gcagtttatt
     1621 actcactaaa gacagaatga atgtagaaaa ggctgaattc tgtaataaaa gcaaacagcc
     1681 tggcttagca aggagccaac ataacagatg ggctggaagt aaggaaacat gtaatgatag
     1741 gcggactccc agcacagaaa aaaaggtaga tctgaatgct gatcccctgt gtgagagaaa
     1801 agaatggaat aagcagaaac tgccatgctc agagaatcct agagatactg aagatgttcc
     1861 ttggataaca ctaaatagca gcattcagaa agttaatgag tggttttcca gaagtgatga
     1921 actgttaggt tctgatgact cacatgatgg ggagtctgaa tcaaatgcca aagtagctga
     1981 tgtattggac gttctaaatg aggtagatga atattctggt tcttcagaga aaatagactt
     2041 actggccagt gatcctcatg aggctttaat atgtaaaagt gaaagagttc actccaaatc
     2101 agtagagagt aatattgaag acaaaatatt tgggaaaacc tatcggaaga aggcaagcct
     2161 ccccaactta agccatgtaa ctgaaaatct aattatagga gcatttgtta ctgagccaca
     2221 gataatacaa gagcgtcccc tcacaaataa attaaagcgt aaaaggagac ctacatcagg
     2281 ccttcatcct gaggatttta tcaagaaagc agatttggca gttcaaaaga ctcctgaaat
     2341 gataaatcag ggaactaacc aaacggagca gaatggtcaa gtgatgaata ttactaatag
     2401 tggtcatgag aataaaacaa aaggtgattc tattcagaat gagaaaaatc ctaacccaat
     2461 agaatcactc gaaaaagaat ctgctttcaa aacgaaagct gaacctataa gcagcagtat
     2521 aagcaatatg gaactcgaat taaatatcca caattcaaaa gcacctaaaa agaataggct
     2581 gaggaggaag tcttctacca ggcatattca tgcgcttgaa ctagtagtca gtagaaatct
     2641 aagcccacct aattgtactg aattgcaaat tgatagttgt tctagcagtg aagagataaa
     2701 gaaaaaaaag tacaaccaaa tgccagtcag gcacagcaga aacctacaac tcatggaagg
     2761 taaagaacct gcaactggag ccaagaagag taacaagcca aatgaacaga caagtaaaag
     2821 acatgacagt gatactttcc cagagctgaa gttaacaaat gcacctggtt cttttactaa
     2881 gtgttcaaat accagtgaac ttaaagaatt tgtcaatcct agccttccaa gagaagaaaa
     2941 agaagagaaa ctagaaacag ttaaagtgtc taataatgct gaagacccca aagatctcat
     3001 gttaagtgga gaaagggttt tgcaaactga aagatctgta gagagtagca gtatttcact
     3061 ggtacctggt actgattatg gcactcagga aagtatctcg ttactggaag ttagcactct
     3121 agggaaggca aaaacagaac caaataaatg tgtgagtcag tgtgcagcat ttgaaaaccc
     3181 caagggacta attcatggtt gttccaaaga taatagaaat gacacagaag gctttaagta
     3241 tccattggga catgaagtta accacagtcg ggaaacaagc atagaaatgg aagaaagtga
     3301 acttgatgct cagtatttgc agaatacatt caaggtttca aagcgccagt catttgctct
     3361 gttttcaaat ccaggaaatg cagaagagga atgtgcaaca ttctctgccc actctgggtc
     3421 cttaaagaaa caaagtccaa aagtcacttt tgaatgtgaa caaaaggaag aaaatcaagg
     3481 aaagaatgag tctaatatca agcctgtaca gacagttaat atcactgcag gctttcctgt
     3541 ggttggtcag aaagataagc cagttgataa tgccaaatgt agtatcaaag gaggctctag
     3601 gttttgtcta tcatctcagt tcagaggcaa cgaaactgga ctcattactc caaataaaca
     3661 tggactttta caaaacccat atcgtatacc accacttttt cccatcaagt catttgttaa
     3721 aactaaatgt aagaaaaatc tgctagagga aaactttgag gaacattcaa tgtcacctga
     3781 aagagaaatg ggaaatgaga acattccaag tacagtgagc acaattagcc gtaataacat
     3841 tagagaaaat gtttttaaag gagccagctc aagcaatatt aatgaagtag gttccagtac
     3901 taatgaagtg ggctccagta ttaatgaaat aggttccagt gatgaaaaca ttcaagcaga
     3961 actaggtaga aacagagggc caaaattgaa tgctatgctt agattagggg ttttgcaacc
     4021 tgaggtctat aaacaaagtc ttcctggaag taattgtaag catcctgaaa taaaaaagca
     4081 agaatatgaa gaagtagttc agactgttaa tacagatttc tctccatatc tgatttcaga
     4141 taacttagaa cagcctatgg gaagtagtca tgcatctcag gtaagtatca aggttacaag
     4201 acaggtttaa ggagaccaat aagctattat tcgtatagga tactttatac gaagttatta
     4261 agttatggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     4321 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4381 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4441 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4501 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4561 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     4621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     4861 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     4921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4981 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5041 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5101 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5161 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5221 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5281 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5341 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5401 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5461 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5521 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5581 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5641 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5701 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5761 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5821 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5881 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5941 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6001 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6061 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6121 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6181 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6241 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6301 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6361 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6421 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6481 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6541 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6601 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6661 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6721 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6781 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6841 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6901 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6961 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7021 ctggcctttt gctcacatgt
//