| 出品公司: | Addgene |
|---|---|
| 载体名称: | pMDLg/pRRE |
| 质粒类型: | 慢病毒系统；第三代慢病毒包装载体，哺乳动物表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8895 bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 备注: | pMDLg/pRRE是第三代慢病毒包装载体。与pMDLg/pRRE载体配套的第三代慢病毒包装质粒包括pRSV-Rev 和 pMD2.G |
| 产品目录号: | 12251 |
| 稳定性: | 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
pMDLg/pRRE载体慢病毒包装质粒基本信息
pMDLg/pRRE质粒图谱慢病毒包装载体图谱和pMDLg/pRRE载体序列慢病毒包装质粒序列多克隆位点信息
pMDLg/pRRE质粒慢病毒包装载体简介
第三代慢病毒包装质粒系统包括有：Gag基因作用是编码病毒粒子主要结构蛋白；Pol基因作用是编码特异性的逆转录酶；RRE基因作用是Rev蛋白的结合位点，有利于RNA的出核转运。pMDLG/pRRE质粒不含有Rev基因。第三代慢病毒包装质粒提供了最大的生物安全性，但是由于需要同时转染四个不同的质粒到产毒细胞中，在操作上也更加复杂。 常用的第三代慢病毒包装质粒组合是: pMDLg/pRRE + pRSV-Rev + pMD2.G. 请注意，大部分的Trono实验室慢病毒载体含有一个野生型的5' LTR 。这种类型的慢病毒载体，只能够使用第二代慢病毒包装质粒进行包装。这是由于野生型的5' LTR必须要有TAT序列，才能够被激活。 如果你想使用第三代慢病毒包装质粒，你的慢病毒目的基因质粒必须含有一个嵌合型的5' LTR片段（例如CCL-, RRL-等基因），在该片段中HIV启动子被CMV和RSV启动子取代了，所以该慢病毒质粒，并不依赖于TAT系统。 含有嵌合型5' LTR的慢病毒质粒，既能够使用第二代慢病毒包装质粒，也能够使用第三代慢病毒包装载体。
pMDLg/pRRE质粒序列慢病毒包装载体序列
LOCUS pMDLg/pRRE 8895 bp DNA SYN
DEFINITION pMDLg/pRRE
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8895
/organism="pMDLg/pRRE"
/mol_type="other DNA"
misc_feature complement(40..59)
/label="SV40pro_F_primer"
promoter 162..738
/label="CMV_immearly_promoter"
misc_feature 241..528
/label="CAG_enhancer"
misc_feature 695..715
/label="CMV_fwd_primer"
promoter 696..765
/label="CMV_promoter"
promoter 708..827
/label="CMV2_promoter"
misc_feature 739..758
/label="pCEP_fwd_primer"
misc_feature 741..765
/label="LNCX_primer"
misc_feature 1015..1398
/label="bGlob_int"
gene 1437..2945
/label="Gag_HIV (variant)"
/gene="Gag_HIV (variant)"
CDS 1437..2945
/label="ORF frame 3"
CDS 3011..5749
/label="ORF frame 2"
misc_feature 5438..5453
/label="cPPT"
CDS 5694..6212
/label="ORF frame 3"
misc_feature 5774..6007
/label="RRE"
rep_origin complement(7126..7745)
/label="pBR322_origin"
gene complement(7900..8760)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(7900..8760)
/label="ORF frame 1"
promoter complement(8802..8830)
/label="AmpR_promoter"
ORIGIN
1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
1381 ctggcccatc actttggcaa agcacgtgag atctgaattc gagatctgcc gccgccatgg
1441 gtgcgagagc gtcagtatta agcgggggag aattagatcg atgggaaaaa attcggttaa
1501 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
1561 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
1621 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
1681 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
1741 acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcagctgaca
1801 caggacacag caatcaggtc agccaaaatt accctatagt gcagaacatc caggggcaaa
1861 tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga
1921 aggctttcag cccagaagtg atacccatgt tttcagcatt atcagaagga gccaccccac
1981 aagatttaaa caccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa
2041 aagagaccat caatgaggaa gctgcagaat gggatagagt gcatccagtg catgcagggc
2101 ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta
2161 ctagtaccct tcaggaacaa ataggatgga tgacacataa tccacctatc ccagtaggag
2221 aaatctataa aagatggata atcctgggat taaataaaat agtaagaatg tatagcccta
2281 ccagcattct ggacataaga caaggaccaa aggaaccctt tagagactat gtagaccgat
2341 tctataaaac tctaagagcc gagcaagctt cacaagaggt aaaaaattgg atgacagaaa
2401 ccttgttggt ccaaaatgcg aacccagatt gtaagactat tttaaaagca ttgggaccag
2461 gagcgacact agaagaaatg atgacagcat gtcagggagt ggggggaccc ggccataaag
2521 caagagtttt ggctgaagca atgagccaag taacaaatcc agctaccata atgatacaga
2581 aaggcaattt taggaaccaa agaaagactg ttaagtgttt caattgtggc aaagaagggc
2641 acatagccaa aaattgcagg gcccctagga aaaagggctg ttggaaatgt ggaaaggaag
2701 gacaccaaat gaaagattgt actgagagac aggctaattt tttagggaag atctggcctt
2761 cccacaaggg aaggccaggg aattttcttc agagcagacc agagccaaca gccccaccag
2821 aagagagctt caggtttggg gaagagacaa caactccctc tcagaagcag gagccgatag
2881 acaaggaact gtatccttta gcttccctca gatcactctt tggcagcgac ccctcgtcac
2941 aataaagata ggggggcaat taaaggaagc tctattagat acaggagcag atgatacagt
3001 attagaagaa atgaatttgc caggaagatg gaaaccaaaa atgatagggg gaattggagg
3061 ttttatcaaa gtaggacagt atgatcagat actcatagaa atctgcggac ataaagctat
3121 aggtacagta ttagtaggac ctacacctgt caacataatt ggaagaaatc tgttgactca
3181 gattggctgc actttaaatt ttcccattag tcctattgag actgtaccag taaaattaaa
3241 gccaggaatg gatggcccaa aagttaaaca atggccattg acagaagaaa aaataaaagc
3301 attagtagaa atttgtacag aaatggaaaa ggaaggaaaa atttcaaaaa ttgggcctga
3361 aaatccatac aatactccag tatttgccat aaagaaaaaa gacagtacta aatggagaaa
3421 attagtagat ttcagagaac ttaataagag aactcaagat ttctgggaag ttcaattagg
3481 aataccacat cctgcagggt taaaacagaa aaaatcagta acagtactgg atgtgggcga
3541 tgcatatttt tcagttccct tagataaaga cttcaggaag tatactgcat ttaccatacc
3601 tagtataaac aatgagacac cagggattag atatcagtac aatgtgcttc cacagggatg
3661 gaaaggatca ccagcaatat tccagtgtag catgacaaaa atcttagagc cttttagaaa
3721 acaaaatcca gacatagtca tctatcaata catggatgat ttgtatgtag gatctgactt
3781 agaaataggg cagcatagaa caaaaataga ggaactgaga caacatctgt tgaggtgggg
3841 atttaccaca ccagacaaaa aacatcagaa agaacctcca ttcctttgga tgggttatga
3901 actccatcct gataaatgga cagtacagcc tatagtgctg ccagaaaagg acagctggac
3961 tgtcaatgac atacagaaat tagtgggaaa attgaattgg gcaagtcaga tttatgcagg
4021 gattaaagta aggcaattat gtaaacttct taggggaacc aaagcactaa cagaagtagt
4081 accactaaca gaagaagcag agctagaact ggcagaaaac agggagattc taaaagaacc
4141 ggtacatgga gtgtattatg acccatcaaa agacttaata gcagaaatac agaagcaggg
4201 gcaaggccaa tggacatatc aaatttatca agagccattt aaaaatctga aaacaggaaa
4261 atatgcaaga atgaagggtg cccacactaa tgatgtgaaa caattaacag aggcagtaca
4321 aaaaatagcc acagaaagca tagtaatatg gggaaagact cctaaattta aattacccat
4381 acaaaaggaa acatgggaag catggtggac agagtattgg caagccacct ggattcctga
4441 gtgggagttt gtcaataccc ctcccttagt gaagttatgg taccagttag agaaagaacc
4501 cataatagga gcagaaactt tctatgtaga tggggcagcc aatagggaaa ctaaattagg
4561 aaaagcagga tatgtaactg acagaggaag acaaaaagtt gtccccctaa cggacacaac
4621 aaatcagaag actgagttac aagcaattca tctagctttg caggattcgg gattagaagt
4681 aaacatagtg acagactcac aatatgcatt gggaatcatt caagcacaac cagataagag
4741 tgaatcagag ttagtcagtc aaataataga gcagttaata aaaaaggaaa aagtctacct
4801 ggcatgggta ccagcacaca aaggaattgg aggaaatgaa caagtagatg ggttggtcag
4861 tgctggaatc aggaaagtac tatttttaga tggaatagat aaggcccaag aagaacatga
4921 gaaatatcac agtaattgga gagcaatggc tagtgatttt aacctaccac ctgtagtagc
4981 aaaagaaata gtagccagct gtgataaatg tcagctaaaa ggggaagcca tgcatggaca
5041 agtagactgt agcccaggaa tatggcagct agattgtaca catttagaag gaaaagttat
5101 cttggtagca gttcatgtag ccagtggata tatagaagca gaagtaattc cagcagagac
5161 agggcaagaa acagcatact tcctcttaaa attagcagga agatggccag taaaaacagt
5221 acatacagac aatggcagca atttcaccag tactacagtt aaggccgcct gttggtgggc
5281 ggggatcaag caggaatttg gcattcccta caatccccaa agtcaaggag taatagaatc
5341 tatgaataaa gaattaaaga aaattatagg acaggtaaga gatcaggctg aacatcttaa
5401 gacagcagta caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
5461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
5521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
5581 agtttggaaa ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag taatacaaga
5641 taatagtgac ataaaagtag tgccaagaag aaaagcaaag atcatcaggg attatggaaa
5701 acagatggca ggtgatgatt gtgtggcaag tagacaggat gaggattaac acatggaatt
5761 ccggagcggc cgcaggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
5821 gcgcagcctc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
5881 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
5941 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
6001 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
6061 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
6121 gggacagaga aattaacaat tacacaagct tccgcggaat tcaccccacc agtgcaggct
6181 gcctatcaga aagtggtggc tggtgtggct aatgccctgg cccacaagtt tcactaagct
6241 cgcttccttg ctgtccaatt tctattaaag gttccttggt tccctaagtc caactactaa
6301 actgggggat attatgaagg gccttgagca tctggattct gcctaataaa aaacatttat
6361 tttcattgca atgatgtatt taaattattt ctgaatattt tactaaaaag ggaatgtggg
6421 aggtcagtgc atttaaaaca taaagaaatg aagagctagt tcaaaccttg ggaaaataca
6481 ctatatctta aactccatga aagaaggtga ggctgcaaac agctaatgca cattggcaac
6541 agccctgatg cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt
6601 ggaggttaaa gtttggctat gctgtatttt acattactta ttgttttagc tgtcctcatg
6661 aatgtctttt cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt
6721 ctcttgctta gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat
6781 ggtttctcct cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact
6841 tgaagaagga aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc
6901 ccccactcac agtgacccgg aatccctcga catggcagtc tagcactagt gcggccgcag
6961 atctgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
7021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
7081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
7141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
7201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
7261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
7321 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
7381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
7441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
7501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
7561 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
7621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
7681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
7741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
7801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
7861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
7921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
7981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
8041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
8101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
8161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
8221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
8281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
8341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
8401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
8461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
8521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
8581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
8641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
8701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
8761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
8821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
8881 agtgccacct gacgt
//
pMDLg/pRRE载体图谱慢病毒包装质粒图谱pdf版和pMDLg/pRRE载体序列慢病毒包装质粒序列等相关资料下载
pMDLg/pRRE质粒慢病毒包装载体应用举例
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