pSLQ1651-sgTelomere(F+E)

价格:1000元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: pSLQ1651-sgTelomere(F+E) 
出品公司: 爱迪基因
目录编号: 51024
存储实验室: Bo Huang, Stanley Qi

载体骨架基本信息
载体名称: pSico
载体抗性: 氨苄霉素
完整质粒大小: 8320 bp
载体修饰: None
载体类型: 哺乳细胞表达,Lentiviral, CRISPR
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 高拷贝
生长条件: 30 ℃

插入基因信息
插入基因名称: Optimized sgRNA
物种来源: H. sapiens (human), M. musculus (mouse)
插入大小: 115 bp
基因又名: sgRNA(F+E)
启动子 mouse U6 promoter
克隆方法: 限制性内切酶
5' 克隆位点: BstXI(未破坏)
3' 克隆位点: XhoI(未破坏)
5' 测序引物 atccgacgcgccatctctag
3' 测序引物 tgcatggcggtaatacggttatc

文献引用方法
材料和方法部分: pSLQ1651-sgTelomere(F+E) was a gift from Bo Huang & Stanley Qi (爱迪基因 plasmid # 51024)
参考文献部分: Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. 10.1016/j.cell.2013.12.001 PubMed 24360272

质粒图谱信息

pSLQ1651-sgTelomere(F+E)质粒图谱

质粒简介

质粒序列

LOCUS       pSLQ1651-sgTelomere(F+E)                8309 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  Lentiviral vector that contains an optimized S. pyogenes sgRNA 
            targeting human telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    pSLQ1651-sgTelomere(F+E)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pSLQ1651-sgTelomere(F+E)|
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(41..60)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        232..611
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        613..811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             829..1009
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2438..2555
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2808..2841
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     2845..2865
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        3160..3463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3464..3667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3701..4297
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3701..3720)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4197..4217
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4307..4360
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4367..4376
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4373..5083
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4518..4536)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4786..4805
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    5103..5136
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    5192..5780
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5245..5265)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5663..5674)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5783..5799)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5784..5800)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6309..6489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6551..7139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6631..6650)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7310..8170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7933..7952
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8171..8275)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
       61 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
      121 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
      181 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      241 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      301 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      361 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      421 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      481 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      541 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      601 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      661 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      721 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      781 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
      841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     2401 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     2461 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2521 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2581 atccagtttg gttagtaccg ggcccgctct agagatccga cgcgccatct ctaggcccgc
     2641 gccggccccc tcgcacggac ttgtgggaga agctcggcta ctcccctgcc ccggttaatt
     2701 tgcatataat atttcctagt aactatagag gcttaatgtg cgataaaaga cagataatct
     2761 gttcttttta atactagcta cattttacat gataggcttg gatttctata acttcgtata
     2821 gcatacatta tacgaagtta taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa
     2881 ttgtgtgttt tgagactata agtatccctt ggagaaccac cttgttggtt agggttaggg
     2941 ttagggttag tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc
     3001 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctcgagta ctaggatcca
     3061 ttaggcggcc gcgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
     3121 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     3181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     3241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     3301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     3361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     3421 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     3481 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     3541 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     3601 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     3661 cagagctggt ttagtgaacc gtcagatccg ctagcccacc atgaccgagt acaagcccac
     3721 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     3781 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     3841 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     3901 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3961 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4021 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4081 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4141 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4201 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4261 accgcgcacc tggtgcatga cccgcaagcc cggtgccgga tcgggagagg gcagaggaag
     4321 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca ccggtcgcca ccatggtgag
     4381 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     4441 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     4501 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     4561 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     4621 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     4681 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     4741 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     4801 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     4861 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     4921 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4981 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     5041 gggccgccac tccaccggcg gcatggacga gctgtacaag taagaattcg tcgagggacc
     5101 taataacttc gtatagcata cattatacga agttatacat gtttaagggt tccggttcca
     5161 ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     5221 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5281 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5341 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5401 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5461 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5521 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5581 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5641 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5701 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5761 cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg
     5821 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     5881 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     5941 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6001 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     6061 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     6121 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     6181 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     6241 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     6301 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6481 tctctagcag catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6541 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6601 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6661 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6721 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6781 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6841 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6901 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6961 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7021 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7081 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7141 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7201 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7261 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7321 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7381 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7441 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7501 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7561 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7621 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7681 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7741 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7801 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7861 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7921 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7981 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8041 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8101 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8161 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8221 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8281 ttccccgaaa agtgccacct gacgtcgac
//

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