pJYS1Ptac

LOCUS       pJYS1Ptac               12273 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pJYS1Ptac
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, 
            Li Y, Wang R, Yang S
  TITLE     CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
  JOURNAL   Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
  PUBMED    28469274
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pJYS1Ptac|
FEATURES             Location/Qualifiers
     source          1..12273
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      414..499
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      591..618
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(4657..5451)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4768..4787)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5378..5397
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      8919..9141
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9189..10139
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     complement(11134..11156)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11145..11161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11169..11197)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        11431..11508
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(11528..11547)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
       61 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
      121 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
      181 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
      241 accgcctctc cccgcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
      301 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
      361 gcaagcgaca ggccatgttt gacagcttat catcgataag ctttctagaa tttaaataaa
      421 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      481 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
      541 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
      601 cctgacggat ggcctttttg cgtttctaca aactcttaag cttgcatgcc tgcaggtcga
      661 cttagttatt gcggttctgg acaaattcaa agtattcttc gttcttaatg acgagattca
      721 gcttcttgcc ttcctggttg ttcttgatgc gacccaggag catgagaccc ttcagaccga
      781 tgtggtaggc gccgtttgca tcagcgtcct gtggcatgtt ctttggagcc tggcgggaat
      841 cgaagaagtt gccgttgacg tcggccactg gggagatgag gtaatccagc tcggtgccgg
      901 tcttggagtt gcgcatctgc aggatggtgt tgagcacaga ggtcagcttt gcgaagaact
      961 tcttgtcgga ttcgccgcag attgcagcct tgatgcactc gccgtggccg tattcgatgg
     1021 agtaatcctt caggagcttc tccagttcct tggttgggta cacttcgcgg gtatcccagt
     1081 tgtggttctt gtcggagttg cggaagttga tgaggcggga gccgaaggat gcgatggtcc
     1141 acttgccctt ggctgcctta tcgccgaagt tcttgtagtc gaaggagaat tcgaagtagc
     1201 ccttatccag gttgtagcag atcttgtcga acttggagaa gaactcctgg gacttggaga
     1261 cggattcgta ctttgggtag agctggttga cgaagccggt cactgggcag atcttagagg
     1321 tgaagccagc tggcacgtag tagatgatgc cggtctgctt gcccatcttc ttgaaggtct
     1381 cgaatggggc ggtcagctgg tatgcacgga ggacgccgcc ggtcttatcg aattcgttgt
     1441 ccttgaacac gaggtagttc agcttctcga tgagcatctt ttccagcttc tggtagacct
     1501 gcttctccac cttgaagcgg ccgcgcttga agccgaagtt caggtcttcg aagaccacga
     1561 ttgcgttgta ctcgatcacc agcttagcga tttcgtggac cacctgggag aggtagcctt
     1621 ccttcatttc cttgatgttg ttgatcttct tccaatcctt gcgagcggaa tcgcggtcct
     1681 tctcgatagc ggccagcttg tcgtggtagt tggtcttcat gcgatcgttg ccgatgatgt
     1741 tgaaggtgtc ctgcttgatg atgttgccct tgccgtcgac cagggtgtag taggcgaggt
     1801 ggcgttcgcc gcgatcgatg gacaggatgt gcacgtcgtt agccttctcc ttcaggagca
     1861 ggttgatttc atcgttgaac ttgttggcgc cggaggactt gaagttgatg gtgattgggc
     1921 agtggaagaa gaacttgtcc tcggtgaagc gcttatcctt gatcaggtcg tactcgaaca
     1981 cggattcctt ctttgggtta tccttgttct tgttggcgat tgcttccttg gctgggtggg
     2041 tgatcttctt tgggatggac tgcttgcggt agaacagctc tgcttcgccg ttgagcttgt
     2101 agaccacatc ctgcaggttg cgttcgtcga agagtgcctt ccagtagagg gtgtgcaggt
     2161 ttgggcggcc cttggagtaa gcggagaagt ccttgttgta gatctggaag aggtacagct
     2221 tgccctggtt gaccacggaa tcgatgtagg actcggagat gttttcgaag gtcagcttgt
     2281 agccctggtt ctccacttcg cggtagaatt catcgatgga gttgtagcgc tgggtatcgg
     2341 agaagcggaa gccgaagtcc ttccactctg ggtgcttgga gatggactgc ttgtagaaat
     2401 cgatgaactt gcggcagtct tcgatgttga actcgaactt ttcgtagccc ttctgtgggg
     2461 agccgttctt ggtgtgggtg gagtggttgc ggatgcgcag gatatcttcg gatgggttgt
     2521 agaacttgat ggactttgcg gagaagaaga cctttgggag catcttgtta gcgcctggga
     2581 gcagcttgta cacgatcttc ttgtagccct cgcccttgtt ttccttgatg gccttatcgt
     2641 cgaagatctt gttgttcttc ttgttcatca cgcccaggta gtacttatcg tccttgatga
     2701 agaggatggc ggtgttatct ggttccttgt tcttgtccca gccgtttgcc agggtggagt
     2761 tctcgaagtt gagcttgaac ttttcatcgg agtatggctt ctgggtgatg tagttgcgga
     2821 tcttgttgta cagtgggacg atgttagcga gttcgaagta gcactcttcg aacaccaggt
     2881 agaagtgctc atccttgtcg aggatgttgg ccttgtcttc ggactgggag atgtggaaga
     2941 tcttcagctt gtggagcagg ttgttggtct gatcgagcag gtccttgatt gccttcacat
     3001 cgtcctcagc ggaggcctgg agcagatcct tcttgccctg gttctggtac ttgatggaga
     3061 tctgggccag gttatccttg ttctgtgcga tttcgtcgaa gatcattggg attgcagcga
     3121 agtttgcgag gatctcttcg aagcggcact gcttatcgat gtcgcggtgc ttgttgaact
     3181 cttcgagagc cagcttgatg gtctcgaggg acaggtactt agccttttcg gtcttcttgg
     3241 cgatcagctc ctgttccttc ttggatgggt tatcgaggtt ctttggggcg atctgctggg
     3301 tgatgtattc caggactgcg gtgccgatca cggagtaatc gtcgaagacc tgctgggaga
     3361 gatcggtcag ggacttgtcg ttcttgaagt agatcttgga gagatccagc ttctgagcct
     3421 tcagatcgtc gaagagcagg gagagggtct ccttgatgga cttctcttcc acggtcttga
     3481 aggctgcgat ctgttcgtag aaggactgca tggtggtgac cacatcggaa tcgtcctcca
     3541 gcttgtcgat gacgaaggac ttggattcgg tgtcggacag gatctgcttg aagagcacgg
     3601 acatcttgta cttcttcagg gtcttatcgt tgatctgctg ggagtagagg ttgatgtact
     3661 cgttgatgcc cttgcgcttg gtgttttcgc cgttgacgaa cttgccgccg atgatggtgt
     3721 tgaacttggt gatgccggac tggttcaggt agttgttgaa gttggcgatc tcgaacactt
     3781 catcgaggga gaagacgcgc tggttcactt cagaggtctt gtaatcgatg tcgaaggtga
     3841 gctcttcggc caggtccttc ttgatctgct cgtagttgat agcttctggg gccttatcct
     3901 tcagggactc gtactttgcc ttgttttcga ggaactttgg caggttatcg tcgacgatgc
     3961 ggtagatgat agaggttggg atatcgttgg aggagtacac gttcttgcgg ttttcgtgga
     4021 agcccttgaa gtaggtggtc cagcccttga aggacttgat gatctccaga gcttcatcga
     4081 tgtcggtgat gtcggagttg gccttgaaga gctcgatgcc gttatccttg gactgcttga
     4141 gccacaggat gagatcggat tcctggccct tctttgcgtc gatcaggttc tggttgaaga
     4201 ggttcttgaa cttctcggaa tccttgatgt attcggagat ctgcttcttg atggtatcct
     4261 tagcggactt gaagtccttc tgcaggttat cgtcatcgga cttcttgagc ttgaagtaga
     4321 catcggagta gttctggagc aggtcctcgg agatgcacac ggaggacagg atctcttcga
     4381 tgaagaactg gtggtacttg tcgatgatct gctttgcctt cttgtaatcc ttagcgcgct
     4441 tttcgtcatc caggatgagg ccgcgtgcct tgatgttttc gagggtcttg ccttggggga
     4501 tcagctcaaa acggagggtc ttggacaggg agtatttatt cacaaactct tggtagatgg
     4561 acatcgttca agtcctttcc aattccacac atggtaccac acgatgatta attgtaaaca
     4621 gctcaggtca tgattccgcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     4681 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     4741 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     4801 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     4861 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     4921 ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     4981 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     5041 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     5101 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     5161 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     5221 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt ggagttcatt
     5281 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     5341 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     5401 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     5461 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     5521 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     5581 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     5641 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     5701 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
     5761 ccggtgaccg cctcgatgat cgccgggtgg gcgtggccca aggaggatca tccagccatt
     5821 cggggtcgtt cactggttcc cctttctgat ttctggcata gaagaacccc cgtgaactgt
     5881 gtggttccgg gggttgctga tttttgcgag acttctcgcg caattcccta gcttaggtga
     5941 aaacaccatg aaacactagg gaaacaccca tgaaacaccc attagggcag tagggcggct
     6001 tcttcgtcta gggcttgcat ttgggcggtg atctggtctt tagcgtgtga aagtgtgtcg
     6061 taggtggcgt gctcaatgca ctcgaacgtc acgtcattta ccgggtcacg gtgggcaaag
     6121 agaactagtg ggttagacat tgttttcctc gttgtcggtg gtggtgagct tttctagccg
     6181 ctcggtaaac gcggcgatca tgaactcttg gaggttttca ccgttctgca tgcctgcgcg
     6241 cttcatgtcc tcacgtagtg ccaaaggaac gcgtgcggtg accacgacgg gcttagcctt
     6301 tgcctgcgct tctagtgctt cgatggtggc ttgtgcctgc gcttgctgcg cctgtagtgc
     6361 ctgttgagct tcttgtagtt gctgttctag ctgtgccttg gttgccatgc tttaagactc
     6421 tagtagcttt cctgcgatat gtcatgcgca tgcgtagcaa acattgtcct gcaactcatt
     6481 cattatgtgc agtgctcctg ttactagtcg tacatactca tatttaccta gtctgcatgc
     6541 agtgcatgca catgcagtca tgtcgtgcta atgtgtaaaa catgtacatg cagattgctg
     6601 ggggtgcagg gggcggagcc accctgtcca tgcggggtgt ggggcttgcc ccgccggtac
     6661 agacagtgag caccggggca cctagtcgcg gatacccccc ctaggtatcg gacacgtaac
     6721 cctcccatgt cgatgcaaat ctttaacatt gagtacgggt aagctggcac gcatagccaa
     6781 gctaggcggc caccaaacac cactaaaaat taatagtccc tagacaagac aaacccccgt
     6841 gcgagctacc aactcatatg cacgggggcc acataacccg aaggggtttc aattgacaac
     6901 catagcacta gctaagacaa cgggcacaac acccgcacaa actcgcactg cgcaaccccg
     6961 cacaacatcg ggtctaggta acactgaaat agaagtgaac acctctaagg aaccgcaggt
     7021 caatgagggt tctaaggtca ctcgcgctag ggcgtggcgt aggcaaaacg tcatgtacaa
     7081 gatcaccaat agtaaggctc tggcggggtg ccataggtgg cgcagggacg aagctgttgc
     7141 ggtgtcctgg tcgtctaacg gtgcttcgca gtttgagggt ctgcaaaact ctcactctcg
     7201 ctgggggtca tctctggctg aattggaagt catgggcgaa cgccgcattg agctggctat
     7261 tgctactaag aatcacttgg cggcgggtgg cgcgctcatg atgtttgtgg gcactgttcg
     7321 acacaaccgc tcacagtcat ttgcgcaggt tgaagcgggt attaagactg cgtactcttc
     7381 gatggtgaaa acatctcagt ggaagaaaga acgtgcacgg tacggggtgg agcacaccta
     7441 tagtgactat gaggtcacag actcttgggc gaacggttgg cacttgcacc gcaacatgct
     7501 gttgttcttg gatcgtccac tgtctgacga tgaactcaag gcgtttgagg attccatgtt
     7561 ttcccgctgg tctgctggtg tggttaaggc cggtatggac gcgccactgc gtgagcacgg
     7621 ggtcaaactt gatcaggtgt ctacctgggg tggagacgct gcgaaaatgg caacctacct
     7681 cgctaagggc atgtctcagg aactgactgg ctccgctact aaaaccgcgt ctaaggggtc
     7741 gtacacgccg tttcagatgt tggatatgtt ggccgatcaa agcgacgccg gcgaggatat
     7801 ggacgctgtt ttggtggctc ggtggcgtga gtatgaggtt ggttctaaaa acctgcgttc
     7861 gtcctggtca cgtggggcta agcgtgcttt gggcattgat tacatagacg ctgatgtacg
     7921 tcgtgaaatg gaagaagaac tgtacaagct cgccggtctg gaagcaccgg aacgggtcga
     7981 atcaacccgc gttgctgttg ctttggtgaa gcccgatgat tggaaactga ttcagtctga
     8041 tttcgcggtt aggcagtacg ttctcgattg cgtggataag gctaaggacg tggccgctgc
     8101 gcaacgtgtc gctaatgagg tgctggcaag tctgggtgtg gattccaccc cgtgcatgat
     8161 cgttatggat gatgtggact tggacgcggt tctgcctact catggggacg ctactaagcg
     8221 tgatctgaat gcggcggtgt tcgcgggtaa tgagcagact attcttcgca cccactaaaa
     8281 gcggcataaa ccccgttcga tattttgtgc gatgaattta tggtcaatgt cgcgggggca
     8341 aactatgatg ggtcttgttg ttggcgtccc ggagcgtaaa ggatctaggt gaagatcctt
     8401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8461 cttgatgata ccgctgcctt actgggtgca ttagccagtc tgaatgacct gtcacgggat
     8521 aatccgaagt ggtcagactg gaaaatcaga gggcaggaac tgctgaacag caaaaagtca
     8581 gatagcacca catagcagac ccgccataaa acgccctgag aagcccgtga cgggcttttc
     8641 ttgtattatg ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt gccatttacc
     8701 cccattcact gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact
     8761 caggtgcctg atggtcggag acaaaaggaa tattcagcga tttgcccgag cttgcgaggg
     8821 tgctacttaa gcctttaggg ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg
     8881 acatcctttt gtaatactgc ggaactgact aaagtagtga gttatacaca gggctgggat
     8941 ctattctttt tatctttttt tattctttct ttattctata aattataacc acttgaatat
     9001 aaacaaaaaa aacacacaaa ggtctagcgg aatttacaga gggtctagca gaatttacaa
     9061 gttttccagc aaaggtctag cagaatttac agatacccac aactcaaagg aaaaggacta
     9121 gtaattatca ttgactagcc catctcaatt ggtatagtga ttaaaatcac ctagaccaat
     9181 tgagatgtat gtctgaatta gttgttttca aagcaaatga actagcgatt agtcgctatg
     9241 acttaacgga gcatgaaacc aagctaattt tatgctgtgt ggcactactc aaccccacga
     9301 ttgaaaaccc tacaaggaaa gaacggacgg tatcgttcac ttataaccaa tacgctcaga
     9361 tgatgaacat cagtagggaa aatgcttatg gtgtattagc taaagcaacc agagagctga
     9421 tgacgagaac tgtggaaatc aggaatcctt tggttaaagg ctttgagatt ttccagtgga
     9481 caaactatgc caagttctca agcgaaaaat tagaattagt ttttagtgaa gagatattgc
     9541 cttatctttt ccagttaaaa aaattcataa aatataatct ggaacatgtt aagtcttttg
     9601 aaaacaaata ctctatgagg atttatgagt ggttattaaa agaactaaca caaaagaaaa
     9661 ctcacaaggc aaatatagag attagccttg atgaatttaa gttcatgtta atgcttgaaa
     9721 ataactacca tgagtttaaa aggcttaacc aatgggtttt gaaaccaata agtaaagatt
     9781 taaacactta cagcaatatg aaattggtgg ttgataagcg aggccgcccg actgatacgt
     9841 tgattttcca agttgaacta gatagacaaa tggatctcgt aaccgaactt gagaacaacc
     9901 agataaaaat gaatggtgac aaaataccaa caaccattac atcagattcc tacctacata
     9961 acggactaag aaaaacacta cacgatgctt taactgcaaa aattcagctc accagttttg
    10021 aggcaaaatt tttgagtgac atgcaaagta agcatgatct caatggttcg ttctcatggc
    10081 tcacgcaaaa acaacgaacc acactagaga acatactggc taaatacgga aggatctgag
    10141 gttcttatgg ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa aagtagaaca
    10201 actgttcacc tgttaacttt caagttggcg gtgcattaca ccgccaggct gaattattcc
    10261 tctgaattat cgattacact gtattccccg gttaatacag aggaatctgc aggatcgatt
    10321 gtcagtggtt ccttttcatc cattgatact gcacgctgga tctcaattga tacgggcaaa
    10381 tatttgaaca ggcgacgaat agccgttttc tttgccattt cttcccagtg agttacccac
    10441 ggcccgttat taccagcttt actcaggctg cgcaccagct caatctgttt gcgcgtcata
    10501 acttcaaact gagtacctcc gtctttcagt cttgcgacag catagacgtg ggtaaccggg
    10561 gcatcttcgt tttctcccgg gcggtgtatt aacttttcat caaggccaaa ttcgaagcta
    10621 aactcgtcac cttcacggac aacacgggct gacaggctgg cgatttgacc agaacggcga
    10681 gccagatcaa tcatgccgcg atagccaatg attagctgaa cgttcttttt accgctcttt
    10741 tcgtttttat taccaaaagg cagtaaatat gcatgaccga gggcgctacc tggctcaagt
    10801 ccgagctgtg aacactgtac gatcgcactg acaaaactca tagtgtcaca gtttcctaac
    10861 gccggaactt tacgaatttc tgtggtggcg atacggatca tacgttcagc cgtcatatgg
    10921 cgtggaagag ctgctgccag ttgctctttc attgatggct ggttaataaa actaatcacg
    10981 tcgctatttt taactgctgc tggtgcacgg tttccctgag ttttttgcag atcggctttt
    11041 gcgattggtg gttgcttagt catggatcct ctagagtcga cctgcaggca tgcaagcttg
    11101 gcgtaatcat ggtcatatct tttaattctg tttcctgtgt gaaattgtta tccgctcaca
    11161 attccacaca ttatacgagc cgatgattaa ttgtcaacag ctcatttcag aatatttgcc
    11221 agaaccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga
    11281 cacgaattat gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct
    11341 gacgccagaa gcattggtgc accgtgcagt cgataagccc ggatcagctt gcaattcgcg
    11401 cgcgaaggcg aagcggcatg catttacgtt gacaccatcg aatggtgcaa aacctttcgc
    11461 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta
    11521 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg
    11581 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag
    11641 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt
    11701 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa
    11761 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc
    11821 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt
    11881 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg
    11941 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa
    12001 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg
    12061 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat
    12121 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc
    12181 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt
    12241 gccaacgatc agatggcgct gggcgcaatg cgc
//