lentiGuide-Puro

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: lentiGuide-Puro 
出品公司: 爱迪基因
目录编号: 52963
存储实验室: Feng Zhang

载体骨架基本信息
载体名称: Custom
载体抗性: 氨苄霉素
空载体大小: 9482 bp
载体类型: 哺乳细胞表达,慢病毒载体,CRISPR
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: S. pyogenes sgRNA cassette
物种来源: 合成
插入大小: 100 bp
启动子 hU6
克隆方法: 限制性内切酶
5' 克隆位点: BsmBI(未破坏)
3' 克隆位点: BsmBI (未破坏)
5' 测序引物 hU6-F
3' 测序引物 hGata4-rev (5'-ATTGTGGATGAATACTGCC-3')

文献引用方法
材料和方法部分: lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963)
参考文献部分: Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047 PubMed 25075903

质粒图谱信息

lentiGuide-Puro载体图谱

质粒简介

质粒序列

LOCUS       lentiGuide-Puro Sequencing Result	10183 bp 	DNA	SYN
DEFINITION  lentiGuide-Puro Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10183
                     /organism="lentiGuide-Puro Sequencing Result"
                     /mol_type="other DNA"
     promoter        171..403
                     /label="hU6_promoter"
     misc_feature    333..352
                     /label="LKO1_5_primer"
     misc_feature    2427..2442
                     /label="cPPT"
     promoter        2686..3857
                     /label="EF1a_promoter"
     CDS             complement(2977..3597)
                     /label="ORF frame 2"
     misc_feature    3805..3825
                     /label="EF1a_fwd_primer"
     gene            3870..4469
                     /label="puro (variant)"
                     /gene="puro (variant)"
     CDS             3870..4469
                     /label="ORF frame 3"
     misc_feature    4487..5062
                     /label="WPRE"
     CDS             4575..5084
                     /label="ORF frame 3"
     CDS             4588..5118
                     /label="ORF frame 1"
     misc_feature    5128..5143
                     /label="cPPT"
     misc_feature    5128..5149
                     /label="U3PPT"
     misc_feature    5145..5197
                     /label="delta_U3"
     misc_feature    5198..5378
                     /label="truncHIV-1_3_LTR"
     misc_feature    5198..5378
                     /label="HIV-1_5_LTR"
     terminator      5459..5578
                     /label="SV40_PA_terminator"
     misc_feature    5547..5566
                     /label="EBV_rev_primer"
     rep_origin      5596..5694
                     /label="SV40_origin"
     misc_feature    5679..5698
                     /label="SV40pro_F_primer"
     promoter        complement(5773..5791)
                     /label="T7_promoter"
     promoter        complement(5801..5817)
                     /label="M13_forward20_primer"
     misc_feature    complement(5810..5832)
                     /label="M13_pUC_fwd_primer"
     misc_feature    5810..5958
                     /label="lacZ_a"
     rep_origin      5976..6282
                     /label="f1_origin"
     promoter        6475..6503
                     /label="AmpR_promoter"
     gene            6545..7405
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             6545..7405
                     /label="ORF frame 2"
     rep_origin      7560..8179
                     /label="pBR322_origin"
     promoter        8488..8517
                     /label="lac_promoter"
     misc_feature    8531..8553
                     /label="M13_pUC_rev_primer"
     promoter        8552..8570
                     /label="M13_reverse_primer"
     promoter        8587..8606
                     /label="T3_promoter"
     misc_feature    8860..9040
                     /label="HIV-1_5_LTR"
     misc_feature    8860..9040
                     /label="truncHIV-1_3_LTR"
     misc_feature    9151..9195
                     /label="HIV-1_psi_pack"
     CDS             9583..275
                     /label="ORF frame 1"
     misc_feature    9705..9938
                     /label="RRE"
ORIGIN
    1 TAACAAATTG GCTGTGGTAT ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT
   61 TAAGAATAGT TTTTGCTGTA CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT
  121 TATCGTTTCA GACCCACCTC CCAACCCCGA GGGGACCCAG AGAGGGCCTA TTTCCCATGA
  181 TTCCTTCATA TTTGCATATA CGATACAAGG CTGTTAGAGA GATAATTAGA ATTAATTTGA
  241 CTGTAAACAC AAAGATATTA GTACAAAATA CGTGACGTAG AAAGTAATAA TTTCTTGGGT
  301 AGTTTGCAGT TTTAAAATTA TGTTTTAAAA TGGACTATCA TATGCTTACC GTAACTTGAA
  361 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACACC GGAGACGGTT
  421 GTAAATGAGC ACACAAAATA CACATGCTAA AATATTATAT TCTATGACCT TTATAAAATC
  481 AACCAAAATC TTCTTTTTAA TAACTTTAGT ATCAATAATT AGAATTTTTA TGTTCCTTTT
  541 TGCAAACTTT TAATAAAAAT GAGCAAAATA AAAAAACGCT AGTTTTAGTA ACTCGCGTTG
  601 TTTTCTTCAC CTTTAATAAT AGCTACTCCA CCACTTGTTC CTAAGCGGTC AGCTCCTGCT
  661 TCAATCATTT TTTGAGCATC TTCAAATGTT CTAACTCCAC CAGCTGCTTT AACTAAAGCA
  721 TTGTCTTTAA CAACTGACTT CATTAGTTTA ACATCTTCAA ATGTTGCACC TGATTTTGAA
  781 AATCCTGTTG ATGTTTTAAC AAATTCTAAT CCAGCTTCAA CAGCTATTTC ACAAGCTTTC
  841 ATGATTTCTT CTTTTGTTAA TAAACAATTT TCCATAATAC ATTTAACAAC ATGTGATCCA
  901 GCTGCTTTTT TTACAGCTTT CATGTCTTCT AAAACTAATT CATAATTTTT GTCTTTTAAT
  961 GCACCAATAT TTAATACCAT ATCAATTTCT GTTGCACCAT CTTTAATTGC TTCAGAAACT
 1021 TCGAATGCTT TTGTAGCTGT TGTGCATGCA CCTAGAGGAA AACCTACAAC ATTTGTTATT
 1081 CCTACATTTG TGCCTTTTAA TAATTCTTTA CAATAGCTTG TTCAATATGA ATTAACACAA
 1141 ACTGTTGCAA AATCAAATTC AATTGCTTCA TCACATAATT GTTTAATTTC AGCTTTCGTA
 1201 GCATCTTGTT TTAATAATGT GTGATCTATA TATTTGTTTA GTTTCATTTT TTCTCCTATA
 1261 TATTCATTTT TAATTTTAAT TCTTTAATAA TTTCGTCTAC TTTAACTTTA GCGTTTTGAA
 1321 CAGATTCACC AACACCTATA AAATAAATTT TTAGTTTAGG TTCAGTTCCA CTTGGGCGAA
 1381 CAGCAAATCA TGACTTATCT TCTAAATAAA ATTTTAGTAA GTCTTGTCCT GGCATATTAT
 1441 ACATTCCATC GATGTAGTCT TCAACATTAA CAACTTTAAG TCCAGCAATT TGAGTTAAGG
 1501 GTGTTGCTCT CAATGATTTC ATTAATGGTT CAATTTTTAA TTTCTTTTCT TCTGGTTTAA
 1561 AATTCAAGTT TAAAGTGAAA GTGTAATATG CACCCATTTC TTTAAATAAA TCTTCTAAAT
 1621 AGTCTACTAA TGTTTTATTT TGTTTTTTAT AAAATCAAGC AGCCTCTGCT ATTAATATAG
 1681 AAGCTTGTAT TCCATCTTTA TCTCTAGCTG AGTCATCAAT TACATATCCA TAACTTTCTT
 1741 CATAAGCAAA AACAAAATTT AATCCGTTAT CTTCTTCTTT AGCAATTTCT CTACCCATTC
 1801 ATTTAAATCC AGTTAAAGTT TTTACAATAT TAACTCCATA TTTTTCATGA GCGATTCTAT
 1861 CACCCAAATC ACTTGTTACA AAACTTGAAT ATAGAGCCGG ATTTTTTGGA ATGCTATTTA
 1921 AGCGTTTTAG ATTTGATAAT TTTCAATCAA TTAAAATTGG TCCTGTTTGA TTTCCATCTA
 1981 ATCTTACAAA ATGACCATCA TGTTTTATTG CCATTCCAAA TCTGTCAGCA TCTGGGTCAT
 2041 TCATAATAAT AATATCTGCA TCATGTTTAA TACCATATTC AAGCGGTATT TTTCATGCAG
 2101 GATCAAATTC TGGATTTGGA TTTACAACAT TTTTAAATGT TTCATCTTCA AATGCATGCT
 2161 CTTCAACCTC AATAACGTTA TATCCTGATT CACGTAATAT TTTTGGGGTA AATTTAGTTC
 2221 CTGTTCCATT AACTGCGCTA AAAATAATTT TTAAATCTTT TTTAGCTTCT TGCTCTTTTT
 2281 TGTACGTCTC TGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA
 2341 CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTAAGCTTG GCGTAACTAG ATCTTGAGAC
 2401 AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG GATTGGGGGG TACAGTGCAG
 2461 GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA CAAAAACAAA
 2521 TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG CAGAGATCCA CTTTGGCGCC
 2581 GGCTCGAGGG GGCCCGGGTG CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA
 2641 GCTAATGGAC CTTCTAGGTC TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG
 2701 CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGATCCG
 2761 GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC
 2821 TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT
 2881 TTCGCAACGG GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG
 2941 GCCTCTTTAC GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG
 3001 TGATTCTTGA TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT
 3061 AAGGAGCCCC TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG
 3121 TGCGAATCTG GTGGCACCTT CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT
 3181 AAAATTTTTG ATGACCTGCT GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG
 3241 GCCAAGATCT GCACACTGGT ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG
 3301 CGTCCCAGCG CACATGTTCG GCGAGGCGGG GCCTGCGAGC GCGGCCACCG AGAATCGGAC
 3361 GGGGGTAGTC TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG CCTCGCGCCG CCGTGTATCG
 3421 CCCCGCCCTG GGCGGCAAGG CTGGCCCGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC
 3481 CGCTTCCCGG CCCTGCTGCA GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG
 3541 CGGGTGAGTC ACCCACACAA AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG
 3601 ACTCCACGGA GTACCGGGCG CCGTCCAGGC ACCTCGATTA GTTCTCGAGC TTTTGGAGTA
 3661 CGTCGTCTTT AGGTTGGGGG GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG
 3721 TGGAGACTGA AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT
 3781 TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC
 3841 CATTTCAGGT GTCGTGACGT ACGGCCACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG
 3901 CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC
 3961 CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG
 4021 AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG
 4081 CCGCCGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA
 4141 TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG
 4201 GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGAGTCT
 4261 CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG
 4321 CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT
 4381 ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT
 4441 GGTGCATGAC CCGCAAGCCC GGTGCCTGAA CGCGTTAAGT CGACAATCAA CCTCTGGATT
 4501 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG
 4561 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT
 4621 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC
 4681 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA
 4741 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC
 4801 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT
 4861 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT
 4921 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC
 4981 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA
 5041 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCGTCGACT TTAAGACCAA TGACTTACAA
 5101 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA
 5161 CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT
 5221 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT
 5281 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC
 5341 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT ACGTATAGTA GTTCATGTCA
 5401 TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT
 5461 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
 5521 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA
 5581 TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT
 5641 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC
 5701 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGGACG
 5761 TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC
 5821 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT
 5881 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
 5941 GCCTGAATGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG
 6001 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
 6061 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC
 6121 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG
 6181 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
 6241 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG
 6301 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
 6361 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG
 6421 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
 6481 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA
 6541 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
 6601 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG
 6661 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG
 6721 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT
 6781 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA
 6841 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
 6901 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC
 6961 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG
 7021 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC
 7081 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT
 7141 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
 7201 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG
 7261 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT
 7321 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG
 7381 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT
 7441 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT
 7501 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA
 7561 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA
 7621 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC
 7681 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA
 7741 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT
 7801 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG
 7861 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG
 7921 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC
 7981 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG
 8041 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT
 8101 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG
 8161 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA
 8221 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG
 8281 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC
 8341 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG
 8401 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG
 8461 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG
 8521 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA
 8581 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTGCAAGCTT AATGTAGTCT
 8641 TATGCAATAC TCTTGTAGTC TTGCAACATG GTAACGATGA GTTAGCAACA TGCCTTACAA
 8701 GGAGAGAAAA AGCACCGTGC ATGCCGATTG GTGGAAGTAA GGTGGTACGA TCGTGCCTTA
 8761 TTAGGAAGGC AACAGACGGG TCTGACATGG ATTGGACGAA CCACTGAATT GCCGCATTGC
 8821 AGAGATATTG TATTTAAGTG CCTAGCTCGA TACATAAACG GGTCTCTCTG GTTAGACCAG
 8881 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC
 8941 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA
 9001 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT
 9061 TGAAAGCGAA AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC
 9121 GCACGGCAAG AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG
 9181 GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC
 9241 GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG
 9301 TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG
 9361 AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC
 9421 TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA
 9481 AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG
 9541 CACAGCAAGC GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA
 9601 AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG
 9661 GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT
 9721 GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG
 9781 GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG
 9841 GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC
 9901 CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA
 9961 AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA
10021 CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC
10081 TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA
10141 TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACA
//

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